Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas

Detalhes bibliográficos
Autor(a) principal: da Silva, Adriano Bortolotti
Data de Publicação: 2016
Outros Autores: dos Reis, Caroline Oliveira, Cazetta, Jairo Osvaldo [UNESP], Carlin, Samira Domingues, Landgraf, Paulo Roberto Corrêa, dos Reis, Marinacunha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.14393/BJ-v32n3a2016-31368
http://hdl.handle.net/11449/228158
Resumo: Micropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L-1 of sucrose, solidified with 6 g. L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L-1). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L-1) promoted higher dry mass accumulation and better control of water loss by plants.
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spelling Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeasEffects of exogenous proline and a natural ventilation system on the in vitro growth of orchidsAbiotic stressCattleya walkerianaMicropropagationMicropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L-1 of sucrose, solidified with 6 g. L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L-1). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L-1) promoted higher dry mass accumulation and better control of water loss by plants.Faculdade de Agronomia da Universidade José do Rosário Vellano UNIFENASUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de TecnologiaAgência Paulista de Tecnologia dos Agronegócios Pólo Regional Centro Oeste UPDUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de TecnologiaUNIFENASUniversidade Estadual Paulista (UNESP)UPDda Silva, Adriano Bortolottidos Reis, Caroline OliveiraCazetta, Jairo Osvaldo [UNESP]Carlin, Samira DominguesLandgraf, Paulo Roberto Corrêados Reis, Marinacunha2022-04-29T07:35:50Z2022-04-29T07:35:50Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article619-626http://dx.doi.org/10.14393/BJ-v32n3a2016-31368Bioscience Journal, v. 32, n. 3, p. 619-626, 2016.1981-31631516-3725http://hdl.handle.net/11449/22815810.14393/BJ-v32n3a2016-313682-s2.0-84971607201Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioscience Journalinfo:eu-repo/semantics/openAccess2022-04-29T07:35:50Zoai:repositorio.unesp.br:11449/228158Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:35:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas
Effects of exogenous proline and a natural ventilation system on the in vitro growth of orchids
title Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas
spellingShingle Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas
da Silva, Adriano Bortolotti
Abiotic stress
Cattleya walkeriana
Micropropagation
title_short Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas
title_full Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas
title_fullStr Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas
title_full_unstemmed Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas
title_sort Efeitos da prolina exógena e sistema de ventilação natural no crescimento in vitro de orquídeas
author da Silva, Adriano Bortolotti
author_facet da Silva, Adriano Bortolotti
dos Reis, Caroline Oliveira
Cazetta, Jairo Osvaldo [UNESP]
Carlin, Samira Domingues
Landgraf, Paulo Roberto Corrêa
dos Reis, Marinacunha
author_role author
author2 dos Reis, Caroline Oliveira
Cazetta, Jairo Osvaldo [UNESP]
Carlin, Samira Domingues
Landgraf, Paulo Roberto Corrêa
dos Reis, Marinacunha
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UNIFENAS
Universidade Estadual Paulista (UNESP)
UPD
dc.contributor.author.fl_str_mv da Silva, Adriano Bortolotti
dos Reis, Caroline Oliveira
Cazetta, Jairo Osvaldo [UNESP]
Carlin, Samira Domingues
Landgraf, Paulo Roberto Corrêa
dos Reis, Marinacunha
dc.subject.por.fl_str_mv Abiotic stress
Cattleya walkeriana
Micropropagation
topic Abiotic stress
Cattleya walkeriana
Micropropagation
description Micropropagation is an alternative to produce orchid plants in large scale. However, this process presents losses during acclimatization. Exogenous proline use in vitro plant tissue culture can reduce the stress of the plant acclimatization phase. We aimed to verify the growth of orchids in different micropropagation systems with the addition of proline in the culture medium. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. Seeds were germinated in MS medium, added 20 g. L-1 of sucrose, solidified with 6 g. L-1 of agar and pH adjusted for 5,8. The cultures were incubated in a growth room with temperature of 24 ± 2 0C, under photoperiod of 16 h. After 5 months, 1-cm long seedlings were placed in a culture vessel according to the treatments, which were composed of two micropropagation systems (conventional and natural ventilation) and three proline concentrations (0, 1, and 2 g·L-1). The experiment was carried out in an entirely randomized design consisting of a 2 × 3 factorial, for a total of 6 treatments, each with 5 replicates. The natural ventilation system with the use of proline (1 g·L-1) promoted higher dry mass accumulation and better control of water loss by plants.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
2022-04-29T07:35:50Z
2022-04-29T07:35:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.14393/BJ-v32n3a2016-31368
Bioscience Journal, v. 32, n. 3, p. 619-626, 2016.
1981-3163
1516-3725
http://hdl.handle.net/11449/228158
10.14393/BJ-v32n3a2016-31368
2-s2.0-84971607201
url http://dx.doi.org/10.14393/BJ-v32n3a2016-31368
http://hdl.handle.net/11449/228158
identifier_str_mv Bioscience Journal, v. 32, n. 3, p. 619-626, 2016.
1981-3163
1516-3725
10.14393/BJ-v32n3a2016-31368
2-s2.0-84971607201
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioscience Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 619-626
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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