Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production

Detalhes bibliográficos
Autor(a) principal: Oliveira, Arildo José Braz de
Data de Publicação: 2008
Outros Autores: Koike, Luzia, Reis, Francisco de Assis Machado, Shepherd, Simone Liliane Kirszenzaft
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2719
Resumo: Callus culture of Aspidosperma ramiflorum was established in Murashige and Skoog medium. Callus were supplemented with 1 mg.L-1 2,4-dichlorophenoxyacetic acid, 1.5 mg.L-1 benzylaminopurine, 30 g L-1 sucrose and 10 g L-1 agar. Cultures accumulated the same major alkaloids, ramiflorine A and ramiflorine B, present in the stem bark of the parent plant. The alkaloid contents of 10-methoxy-geissoschizol, ramiflorine A and ramiflorine B, of bark and callus cultures were quantitatively compared by HPLC
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spelling Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid productionCultura de calos de Aspidosperma ramiflorum Muell. Arg.: crescimento e produção de alcalóidesApocynaceaeAspidosperma ramiflorumalcalóides monoterpenóidesramiflorina Aramiflorina Bcultura de tecidos2.00.00.00-6 Ciências BiológicasCallus culture of Aspidosperma ramiflorum was established in Murashige and Skoog medium. Callus were supplemented with 1 mg.L-1 2,4-dichlorophenoxyacetic acid, 1.5 mg.L-1 benzylaminopurine, 30 g L-1 sucrose and 10 g L-1 agar. Cultures accumulated the same major alkaloids, ramiflorine A and ramiflorine B, present in the stem bark of the parent plant. The alkaloid contents of 10-methoxy-geissoschizol, ramiflorine A and ramiflorine B, of bark and callus cultures were quantitatively compared by HPLCA cultura de calos de Aspidosperma ramiflorum foi estabelecida em meio MS. Os calos foram suplementados com 1mg.L-1 de 2,4-dichlorophenoxyacetic ácido, 1.5 mg.L-1 benzylaminopurine, 30 g.L-1 de sacarose e 10 g.L-1 de agar. As culturas acumularam os mesmos alcalóides principais, como ramiflorina A e ramiflorina B, presentes nos galhos da planta adulta. Os conteúdo alcaloídico de 10-metoxi-geissoschizol, ramiflorina A e ramiflorina B, das cascas do tronco e das culturas de calos foram comparados quantitativamente por HPLCUniversidade Estadual De Maringá2008-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/271910.4025/actascibiolsci.v23i0.2719Acta Scientiarum. Biological Sciences; Vol 23 (2001); 609-612Acta Scientiarum. Biological Sciences; v. 23 (2001); 609-6121807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2719/2039Oliveira, Arildo José Braz deKoike, LuziaReis, Francisco de Assis MachadoShepherd, Simone Liliane Kirszenzaftinfo:eu-repo/semantics/openAccess2022-11-23T17:33:28Zoai:periodicos.uem.br/ojs:article/2719Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:28Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production
Cultura de calos de Aspidosperma ramiflorum Muell. Arg.: crescimento e produção de alcalóides
title Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production
spellingShingle Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production
Oliveira, Arildo José Braz de
Apocynaceae
Aspidosperma ramiflorum
alcalóides monoterpenóides
ramiflorina A
ramiflorina B
cultura de tecidos
2.00.00.00-6 Ciências Biológicas
title_short Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production
title_full Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production
title_fullStr Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production
title_full_unstemmed Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production
title_sort Callus culture of Aspidosperma ramiflorum Muell. Arg.: growth and alkaloid production
author Oliveira, Arildo José Braz de
author_facet Oliveira, Arildo José Braz de
Koike, Luzia
Reis, Francisco de Assis Machado
Shepherd, Simone Liliane Kirszenzaft
author_role author
author2 Koike, Luzia
Reis, Francisco de Assis Machado
Shepherd, Simone Liliane Kirszenzaft
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Arildo José Braz de
Koike, Luzia
Reis, Francisco de Assis Machado
Shepherd, Simone Liliane Kirszenzaft
dc.subject.por.fl_str_mv Apocynaceae
Aspidosperma ramiflorum
alcalóides monoterpenóides
ramiflorina A
ramiflorina B
cultura de tecidos
2.00.00.00-6 Ciências Biológicas
topic Apocynaceae
Aspidosperma ramiflorum
alcalóides monoterpenóides
ramiflorina A
ramiflorina B
cultura de tecidos
2.00.00.00-6 Ciências Biológicas
description Callus culture of Aspidosperma ramiflorum was established in Murashige and Skoog medium. Callus were supplemented with 1 mg.L-1 2,4-dichlorophenoxyacetic acid, 1.5 mg.L-1 benzylaminopurine, 30 g L-1 sucrose and 10 g L-1 agar. Cultures accumulated the same major alkaloids, ramiflorine A and ramiflorine B, present in the stem bark of the parent plant. The alkaloid contents of 10-methoxy-geissoschizol, ramiflorine A and ramiflorine B, of bark and callus cultures were quantitatively compared by HPLC
publishDate 2008
dc.date.none.fl_str_mv 2008-05-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2719
10.4025/actascibiolsci.v23i0.2719
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2719
identifier_str_mv 10.4025/actascibiolsci.v23i0.2719
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2719/2039
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 23 (2001); 609-612
Acta Scientiarum. Biological Sciences; v. 23 (2001); 609-612
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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