Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize

Detalhes bibliográficos
Autor(a) principal: Sekine, Elizabete Satsuki
Data de Publicação: 2008
Outros Autores: Castro, Laura Leal de, Prioli, Alberto José, Prioli, Sônia Maria Alves Pinto, Prioli, Laudenir Maria
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2818
Resumo: In maize (Zea mays L.), the Texas (T) cytoplasm carries male sterility (cms-T) and susceptibility to host-specific fungal toxins and to the insecticide methomyl, traits of great interest both for basic research and plant breeding. These traits are apparently inseparable, and they are directly associated with the T-urf13 mitochondrial gene, which encodes a 13-kilodalton protein (URF13). Reversion to male fertility, associated with toxin/methomyl resistance, has been observed in progenies of cms-T somaclones regenerated from methomyl resistant calli. Diversity for PCR amplification of T-urf13 sequence and URF13 expression was observed within revertant progenies. Apparently, this diversity occurred among tissues within ear and tassel, and between ears and tassels of individual plants. The results demonstrate heteroplasmy in T-urf13 sequence persisting through several generations of T-cytoplasm mutants, that apparently consisted of deletions and sequence alterations. Furthermore, URF13 deficiency suggested that total or partial cytoplasmic reversions to male fertility in cms-T somaclones are caused by T-urf13 heteroplasmy
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spelling Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maizeHeteroplasmia para o gene mitocondrial T-urf13 do citoplasma texas de milhoheteroplasmiamilhoT-urf13esterilidade masculina citoplasmática Texas2.00.00.00-6 Ciências BiológicasIn maize (Zea mays L.), the Texas (T) cytoplasm carries male sterility (cms-T) and susceptibility to host-specific fungal toxins and to the insecticide methomyl, traits of great interest both for basic research and plant breeding. These traits are apparently inseparable, and they are directly associated with the T-urf13 mitochondrial gene, which encodes a 13-kilodalton protein (URF13). Reversion to male fertility, associated with toxin/methomyl resistance, has been observed in progenies of cms-T somaclones regenerated from methomyl resistant calli. Diversity for PCR amplification of T-urf13 sequence and URF13 expression was observed within revertant progenies. Apparently, this diversity occurred among tissues within ear and tassel, and between ears and tassels of individual plants. The results demonstrate heteroplasmy in T-urf13 sequence persisting through several generations of T-cytoplasm mutants, that apparently consisted of deletions and sequence alterations. Furthermore, URF13 deficiency suggested that total or partial cytoplasmic reversions to male fertility in cms-T somaclones are caused by T-urf13 heteroplasmyEm milho (Zea mays L.), o citoplasma texas (T) condiciona macho-esterilidade (cms-T) e susceptibilidade à toxina de fungos e ao inseticida metomil, características de grande interesse para estudos básicos e melhoramento de plantas. Essas características são aparentemente inseparáveis e estão diretamente associadas ao gene mitocondrial T-urf13, que codifica uma proteína de 13 kilodaltons (URF13). Reversão para fertilidade masculina, associada à resistência à toxina/metomil, tem sido observada em progênies de somaclones cms-T, derivados de calos resistentes ao metomil. Foi encontrada diversidade para amplificação via PCR do gene T-urf13 e para expressão da proteína URF13 nesses somaclones revertentes. Aparentemente, esta diversidade ocorre dentro de tecidos de espiga e panícula e entre espiga e panícula de uma mesma planta. Os resultados demonstram heteroplasmia na seqüência T-urf13, que persistiu em várias gerações desses mutantes, consistindo aparentemente de deleções e alterações na seqüência de DNA. Além disso, deficiência da proteína URF13 em somaclones férteis sugeriu que a reversão total ou parcial da macho-fertilidade é causada pela heteroplasmia no gene T-urf13Universidade Estadual De Maringá2008-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/281810.4025/actascibiolsci.v22i0.2818Acta Scientiarum. Biological Sciences; Vol 22 (2000); 287-295Acta Scientiarum. Biological Sciences; v. 22 (2000); 287-2951807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2818/2091Sekine, Elizabete SatsukiCastro, Laura Leal dePrioli, Alberto JoséPrioli, Sônia Maria Alves PintoPrioli, Laudenir Mariainfo:eu-repo/semantics/openAccess2022-11-23T17:33:30Zoai:periodicos.uem.br/ojs:article/2818Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:30Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize
Heteroplasmia para o gene mitocondrial T-urf13 do citoplasma texas de milho
title Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize
spellingShingle Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize
Sekine, Elizabete Satsuki
heteroplasmia
milho
T-urf13
esterilidade masculina citoplasmática Texas
2.00.00.00-6 Ciências Biológicas
title_short Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize
title_full Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize
title_fullStr Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize
title_full_unstemmed Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize
title_sort Heteroplasmy in the T-urf13 mitochondrial gene of the Texas cytoplasm of maize
author Sekine, Elizabete Satsuki
author_facet Sekine, Elizabete Satsuki
Castro, Laura Leal de
Prioli, Alberto José
Prioli, Sônia Maria Alves Pinto
Prioli, Laudenir Maria
author_role author
author2 Castro, Laura Leal de
Prioli, Alberto José
Prioli, Sônia Maria Alves Pinto
Prioli, Laudenir Maria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Sekine, Elizabete Satsuki
Castro, Laura Leal de
Prioli, Alberto José
Prioli, Sônia Maria Alves Pinto
Prioli, Laudenir Maria
dc.subject.por.fl_str_mv heteroplasmia
milho
T-urf13
esterilidade masculina citoplasmática Texas
2.00.00.00-6 Ciências Biológicas
topic heteroplasmia
milho
T-urf13
esterilidade masculina citoplasmática Texas
2.00.00.00-6 Ciências Biológicas
description In maize (Zea mays L.), the Texas (T) cytoplasm carries male sterility (cms-T) and susceptibility to host-specific fungal toxins and to the insecticide methomyl, traits of great interest both for basic research and plant breeding. These traits are apparently inseparable, and they are directly associated with the T-urf13 mitochondrial gene, which encodes a 13-kilodalton protein (URF13). Reversion to male fertility, associated with toxin/methomyl resistance, has been observed in progenies of cms-T somaclones regenerated from methomyl resistant calli. Diversity for PCR amplification of T-urf13 sequence and URF13 expression was observed within revertant progenies. Apparently, this diversity occurred among tissues within ear and tassel, and between ears and tassels of individual plants. The results demonstrate heteroplasmy in T-urf13 sequence persisting through several generations of T-cytoplasm mutants, that apparently consisted of deletions and sequence alterations. Furthermore, URF13 deficiency suggested that total or partial cytoplasmic reversions to male fertility in cms-T somaclones are caused by T-urf13 heteroplasmy
publishDate 2008
dc.date.none.fl_str_mv 2008-05-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2818
10.4025/actascibiolsci.v22i0.2818
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2818
identifier_str_mv 10.4025/actascibiolsci.v22i0.2818
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2818/2091
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 22 (2000); 287-295
Acta Scientiarum. Biological Sciences; v. 22 (2000); 287-295
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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