Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612 |
Resumo: | Total proteins and esterases from silk gland extracts of Bombyx mori silkworm were characterized and compared with electrophoretic profiles of prepared extracts with silkworm glands infected with nucleopolyhedrovirus (BmNPV). SDS-PAGE (7%) gels was used for the total proteins, and 10% native PAGE for esterases. In the silk glands extracts of healthy silkworms, it was observed seven protein zones with molecular weight varying between 10 kDa (P1) and 60 kDa (P7). In the infected silkworms, a new zone named P8 (90 kDa) was also detected. Esterases activity at 5th instar larvae underwent changes after the infection with BmNPV, since there was a reduction (EST-6 and EST-7) and an increase (EST-8) in the intensity of the regions of esterases activity, and specificity of EST-9 to β-naphthyl acetate. Those alterations observed in the expression of genes after the infection with the nucleoplyhedrovirus can be used as markers to detect infections in B. mori. |
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Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612viral infectionsilk glandisoenzymesSDS-PAGEmolecular markers2.02.02.00-8Total proteins and esterases from silk gland extracts of Bombyx mori silkworm were characterized and compared with electrophoretic profiles of prepared extracts with silkworm glands infected with nucleopolyhedrovirus (BmNPV). SDS-PAGE (7%) gels was used for the total proteins, and 10% native PAGE for esterases. In the silk glands extracts of healthy silkworms, it was observed seven protein zones with molecular weight varying between 10 kDa (P1) and 60 kDa (P7). In the infected silkworms, a new zone named P8 (90 kDa) was also detected. Esterases activity at 5th instar larvae underwent changes after the infection with BmNPV, since there was a reduction (EST-6 and EST-7) and an increase (EST-8) in the intensity of the regions of esterases activity, and specificity of EST-9 to β-naphthyl acetate. Those alterations observed in the expression of genes after the infection with the nucleoplyhedrovirus can be used as markers to detect infections in B. mori. Universidade Estadual De Maringá2012-07-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionGenética Bioquímicaapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/861210.4025/actascibiolsci.v35i1.8612Acta Scientiarum. Biological Sciences; Vol 35 No 1 (2013); 77-82Acta Scientiarum. Biological Sciences; v. 35 n. 1 (2013); 77-821807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612/pdf_1Ruiz, Juliana BuenoRonqui, LudimillaOliveira, Jussara Ricardo deRuvolo-Takasusuki, Maria Claudia Collainfo:eu-repo/semantics/openAccess2022-11-23T17:34:00Zoai:periodicos.uem.br/ojs:article/8612Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:34Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 |
title |
Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 |
spellingShingle |
Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 Ruiz, Juliana Bueno viral infection silk gland isoenzymes SDS-PAGE molecular markers 2.02.02.00-8 |
title_short |
Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 |
title_full |
Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 |
title_fullStr |
Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 |
title_full_unstemmed |
Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 |
title_sort |
Comparative electrophoretic profile of proteins and esterases in healthy silkworm larvae (Bombyx mori Lineu, 1758) and infected with nucleopolyhedrovirus - doi: 10.4025/actascibiolsci.v35i1.8612 |
author |
Ruiz, Juliana Bueno |
author_facet |
Ruiz, Juliana Bueno Ronqui, Ludimilla Oliveira, Jussara Ricardo de Ruvolo-Takasusuki, Maria Claudia Colla |
author_role |
author |
author2 |
Ronqui, Ludimilla Oliveira, Jussara Ricardo de Ruvolo-Takasusuki, Maria Claudia Colla |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ruiz, Juliana Bueno Ronqui, Ludimilla Oliveira, Jussara Ricardo de Ruvolo-Takasusuki, Maria Claudia Colla |
dc.subject.por.fl_str_mv |
viral infection silk gland isoenzymes SDS-PAGE molecular markers 2.02.02.00-8 |
topic |
viral infection silk gland isoenzymes SDS-PAGE molecular markers 2.02.02.00-8 |
description |
Total proteins and esterases from silk gland extracts of Bombyx mori silkworm were characterized and compared with electrophoretic profiles of prepared extracts with silkworm glands infected with nucleopolyhedrovirus (BmNPV). SDS-PAGE (7%) gels was used for the total proteins, and 10% native PAGE for esterases. In the silk glands extracts of healthy silkworms, it was observed seven protein zones with molecular weight varying between 10 kDa (P1) and 60 kDa (P7). In the infected silkworms, a new zone named P8 (90 kDa) was also detected. Esterases activity at 5th instar larvae underwent changes after the infection with BmNPV, since there was a reduction (EST-6 and EST-7) and an increase (EST-8) in the intensity of the regions of esterases activity, and specificity of EST-9 to β-naphthyl acetate. Those alterations observed in the expression of genes after the infection with the nucleoplyhedrovirus can be used as markers to detect infections in B. mori. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Genética Bioquímica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612 10.4025/actascibiolsci.v35i1.8612 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612 |
identifier_str_mv |
10.4025/actascibiolsci.v35i1.8612 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8612/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 35 No 1 (2013); 77-82 Acta Scientiarum. Biological Sciences; v. 35 n. 1 (2013); 77-82 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317394974310400 |