In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52866 |
Resumo: | In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff. |
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Acta Scientiarum Biological Sciences |
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In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations auxin; cytokinin; cassava; micropropagation; phytohormones.In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff.Universidade Estadual De Maringá2021-04-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5286610.4025/actascibiolsci.v43i1.52866Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e52866Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e528661807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52866/751375151971Copyright (c) 2021 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Karen Cristina Fialho dos Silveira, Deyse Maria de Souza Souza, Antônio da Silva Sá, Jucieny Ferreira de Ledo, Carlos Alberto da SilvaCarvalho, Mariane de Jesus da Silva de 2022-02-17T21:58:54Zoai:periodicos.uem.br/ojs:article/52866Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T21:58:54Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations |
title |
In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations |
spellingShingle |
In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations Santos, Karen Cristina Fialho dos auxin; cytokinin; cassava; micropropagation; phytohormones. |
title_short |
In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations |
title_full |
In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations |
title_fullStr |
In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations |
title_full_unstemmed |
In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations |
title_sort |
In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations |
author |
Santos, Karen Cristina Fialho dos |
author_facet |
Santos, Karen Cristina Fialho dos Silveira, Deyse Maria de Souza Souza, Antônio da Silva Sá, Jucieny Ferreira de Ledo, Carlos Alberto da Silva Carvalho, Mariane de Jesus da Silva de |
author_role |
author |
author2 |
Silveira, Deyse Maria de Souza Souza, Antônio da Silva Sá, Jucieny Ferreira de Ledo, Carlos Alberto da Silva Carvalho, Mariane de Jesus da Silva de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Karen Cristina Fialho dos Silveira, Deyse Maria de Souza Souza, Antônio da Silva Sá, Jucieny Ferreira de Ledo, Carlos Alberto da Silva Carvalho, Mariane de Jesus da Silva de |
dc.subject.por.fl_str_mv |
auxin; cytokinin; cassava; micropropagation; phytohormones. |
topic |
auxin; cytokinin; cassava; micropropagation; phytohormones. |
description |
In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52866 10.4025/actascibiolsci.v43i1.52866 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52866 |
identifier_str_mv |
10.4025/actascibiolsci.v43i1.52866 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/52866/751375151971 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e52866 Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e52866 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317397848457216 |