Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571

Detalhes bibliográficos
Autor(a) principal: Oliveira, Luana Paula de Azevedo de
Data de Publicação: 2016
Outros Autores: Oliveira, Tiago Felipe, Contiero, Jonas, Cazetta, Márcia Luciana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489
Resumo: The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours. 
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spelling Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571yeastmicrobial enzymesagricultural substrateMicrobiologia aplicadaThe factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours. Universidade Estadual De Maringá2016-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionfermentaçãoapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3148910.4025/actascibiolsci.v38i3.31489Acta Scientiarum. Biological Sciences; Vol 38 No 3 (2016); 283-289Acta Scientiarum. Biological Sciences; v. 38 n. 3 (2016); 283-2891807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489/pdfCopyright (c) 2016 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessOliveira, Luana Paula de Azevedo deOliveira, Tiago FelipeContiero, JonasCazetta, Márcia Luciana2022-02-20T22:00:34Zoai:periodicos.uem.br/ojs:article/31489Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-20T22:00:34Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
title Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
spellingShingle Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
Oliveira, Luana Paula de Azevedo de
yeast
microbial enzymes
agricultural substrate
Microbiologia aplicada
title_short Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
title_full Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
title_fullStr Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
title_full_unstemmed Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
title_sort Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
author Oliveira, Luana Paula de Azevedo de
author_facet Oliveira, Luana Paula de Azevedo de
Oliveira, Tiago Felipe
Contiero, Jonas
Cazetta, Márcia Luciana
author_role author
author2 Oliveira, Tiago Felipe
Contiero, Jonas
Cazetta, Márcia Luciana
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Luana Paula de Azevedo de
Oliveira, Tiago Felipe
Contiero, Jonas
Cazetta, Márcia Luciana
dc.subject.por.fl_str_mv yeast
microbial enzymes
agricultural substrate
Microbiologia aplicada
topic yeast
microbial enzymes
agricultural substrate
Microbiologia aplicada
description The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours. 
publishDate 2016
dc.date.none.fl_str_mv 2016-12-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
fermentação
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489
10.4025/actascibiolsci.v38i3.31489
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489
identifier_str_mv 10.4025/actascibiolsci.v38i3.31489
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31489/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2016 Acta Scientiarum. Biological Sciences
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Acta Scientiarum. Biological Sciences
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 38 No 3 (2016); 283-289
Acta Scientiarum. Biological Sciences; v. 38 n. 3 (2016); 283-289
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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