Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223 |
Resumo: | Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions. |
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Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, AmazonasbiodegradationcharacterizationDCPIPantimicrobial resistance.MicrobiologiaMicrobiologiaSerratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions. Universidade Estadual De Maringá2017-11-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBiodegradaçãoBiodegradação,application/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3622310.4025/actascibiolsci.v39i4.36223Acta Scientiarum. Biological Sciences; Vol 39 No 4 (2017); 489-496Acta Scientiarum. Biological Sciences; v. 39 n. 4 (2017); 489-4961807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223/pdfCopyright (c) 2017 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessPeixoto, Ferdyanne Beatriz SabóiaPeixoto, Jean Charles da CunhaAssunção, Enedina Nogueira dePeixoto, Elise MirandaPereira, José OdairAstolfi-Filho, Spartaco2022-02-17T22:00:02Zoai:periodicos.uem.br/ojs:article/36223Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T22:00:02Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas |
title |
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas |
spellingShingle |
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas Peixoto, Ferdyanne Beatriz Sabóia biodegradation characterization DCPIP antimicrobial resistance. Microbiologia Microbiologia |
title_short |
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas |
title_full |
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas |
title_fullStr |
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas |
title_full_unstemmed |
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas |
title_sort |
Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas |
author |
Peixoto, Ferdyanne Beatriz Sabóia |
author_facet |
Peixoto, Ferdyanne Beatriz Sabóia Peixoto, Jean Charles da Cunha Assunção, Enedina Nogueira de Peixoto, Elise Miranda Pereira, José Odair Astolfi-Filho, Spartaco |
author_role |
author |
author2 |
Peixoto, Jean Charles da Cunha Assunção, Enedina Nogueira de Peixoto, Elise Miranda Pereira, José Odair Astolfi-Filho, Spartaco |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Peixoto, Ferdyanne Beatriz Sabóia Peixoto, Jean Charles da Cunha Assunção, Enedina Nogueira de Peixoto, Elise Miranda Pereira, José Odair Astolfi-Filho, Spartaco |
dc.subject.por.fl_str_mv |
biodegradation characterization DCPIP antimicrobial resistance. Microbiologia Microbiologia |
topic |
biodegradation characterization DCPIP antimicrobial resistance. Microbiologia Microbiologia |
description |
Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Biodegradação Biodegradação, |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223 10.4025/actascibiolsci.v39i4.36223 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223 |
identifier_str_mv |
10.4025/actascibiolsci.v39i4.36223 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Acta Scientiarum. Biological Sciences info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Acta Scientiarum. Biological Sciences |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 39 No 4 (2017); 489-496 Acta Scientiarum. Biological Sciences; v. 39 n. 4 (2017); 489-496 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317396947730432 |