Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas

Detalhes bibliográficos
Autor(a) principal: Peixoto, Ferdyanne Beatriz Sabóia
Data de Publicação: 2017
Outros Autores: Peixoto, Jean Charles da Cunha, Assunção, Enedina Nogueira de, Peixoto, Elise Miranda, Pereira, José Odair, Astolfi-Filho, Spartaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223
Resumo: Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions. 
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spelling Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, AmazonasbiodegradationcharacterizationDCPIPantimicrobial resistance.MicrobiologiaMicrobiologiaSerratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions. Universidade Estadual De Maringá2017-11-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBiodegradaçãoBiodegradação,application/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3622310.4025/actascibiolsci.v39i4.36223Acta Scientiarum. Biological Sciences; Vol 39 No 4 (2017); 489-496Acta Scientiarum. Biological Sciences; v. 39 n. 4 (2017); 489-4961807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223/pdfCopyright (c) 2017 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessPeixoto, Ferdyanne Beatriz SabóiaPeixoto, Jean Charles da CunhaAssunção, Enedina Nogueira dePeixoto, Elise MirandaPereira, José OdairAstolfi-Filho, Spartaco2022-02-17T22:00:02Zoai:periodicos.uem.br/ojs:article/36223Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T22:00:02Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
title Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
spellingShingle Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
Peixoto, Ferdyanne Beatriz Sabóia
biodegradation
characterization
DCPIP
antimicrobial resistance.
Microbiologia
Microbiologia
title_short Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
title_full Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
title_fullStr Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
title_full_unstemmed Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
title_sort Petroleum biodegrading and co-resistance to antibiotics by Serratia marcescens strain isolated in Coari, Amazonas
author Peixoto, Ferdyanne Beatriz Sabóia
author_facet Peixoto, Ferdyanne Beatriz Sabóia
Peixoto, Jean Charles da Cunha
Assunção, Enedina Nogueira de
Peixoto, Elise Miranda
Pereira, José Odair
Astolfi-Filho, Spartaco
author_role author
author2 Peixoto, Jean Charles da Cunha
Assunção, Enedina Nogueira de
Peixoto, Elise Miranda
Pereira, José Odair
Astolfi-Filho, Spartaco
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Peixoto, Ferdyanne Beatriz Sabóia
Peixoto, Jean Charles da Cunha
Assunção, Enedina Nogueira de
Peixoto, Elise Miranda
Pereira, José Odair
Astolfi-Filho, Spartaco
dc.subject.por.fl_str_mv biodegradation
characterization
DCPIP
antimicrobial resistance.
Microbiologia
Microbiologia
topic biodegradation
characterization
DCPIP
antimicrobial resistance.
Microbiologia
Microbiologia
description Serratia marcescens is a Gram-negative bacillus, anaerobic facultative belonging to the family Enterobacteriaceae. S. marcescens strains are able to grow in the presence of different xenobiotic compounds, among them, petroleum and heavy metals. Xenobiotic resistant strains develop concomitant resistance to multiple antibiotics, referred to as co-resistance. The AMS212 strain was submitted to the microplate qualitative DCPIP - redox 2,6 dichlorophenol indophenol method. The quantitative test was carried out in Erlenmeyer flasks, followed by the change of color with the absorbance readings, trough the colorimetric method. The antibiotic resistance profile was evaluated by the Kirby-Bauer method. In the qualitative assay, the AMS212 strain altered the color of the DCPIP, which changed from blue to colorless, confirming that petroleum biodegradation occurred. In the quantitative test, the readings were decreasing, confirming that the concentration of DCPIP decreased as a function of the incubation time. The susceptibility test revealed that the AMS212 strain presented multiresistance to four different antibiotics. S. marcescens presented high performance in the biodegradation of petroleum, opening possibility to use it in projects involving the remediation of impacted areas. The expression of the antibiotic co-resistance phenotype confirms that the AMS212 strain is able to withstand different environmental aggressions. 
publishDate 2017
dc.date.none.fl_str_mv 2017-11-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Biodegradação
Biodegradação,
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223
10.4025/actascibiolsci.v39i4.36223
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223
identifier_str_mv 10.4025/actascibiolsci.v39i4.36223
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36223/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2017 Acta Scientiarum. Biological Sciences
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Acta Scientiarum. Biological Sciences
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 39 No 4 (2017); 489-496
Acta Scientiarum. Biological Sciences; v. 39 n. 4 (2017); 489-496
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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