Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624

Detalhes bibliográficos
Autor(a) principal: Santos, Janilza da Paixão
Data de Publicação: 2008
Outros Autores: Dornelles, Ana Lúcia Cunha de, Pereira, Flávia Dionísio, Oliveira, Lenaldo Muniz
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3624
Resumo: Sempre-viva-de-mucugê [Syngonanthus mucugensis – Eriocaulaceae] is a plant with great ornamental value characterized by the durability of its inflorescences, which remains even after collected and dried. The sexual propagation of this species results in disuniform plants, as it is genetically segregating; in addition, this species is currently endangered, with collection therefore restricted to its region of origin. Thus, tissue culture becomes a viable alternative for the formation of new plants. This study aimed to evaluate the in vitro induction of callus in Syngonanthus mucugensis using different BAP concentrations. The experimental design was completely randomized, with each treatment containing 4 repetitions and each repetition formed by 4 explants. The explants used were seedlings cultivated in vitro and their nodal segments. The culture medium used was MS (half-strength concentrations) supplemented with 0.0; 0.89; 1.78; 3.55; 7.10; 14.21 and 28.42 µM of BAP. At 60 days, the survival of the explants and the percentage of formation of callus were evaluated. It was verified that seedlings and nodal segments of Syngonanthus mucugensis are responsive explants to the formation of friable callus, with significant production obtained using the 1.78 and 3.55 µM BAP concentrations.
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spelling Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624Indução de calos em sempre-viva (Syngonanthus mucugensis Giulietti), utilizando diferentes tipos de explantes e concentrações de BAP - DOI: 10.4025/actascibiolsci.v30i2.3624Syngonanthus mucugensisEriocaulaceaecultura de tecidosregulador de crescimentoplanta ornamental2.03.00.00-0 BotânicaSempre-viva-de-mucugê [Syngonanthus mucugensis – Eriocaulaceae] is a plant with great ornamental value characterized by the durability of its inflorescences, which remains even after collected and dried. The sexual propagation of this species results in disuniform plants, as it is genetically segregating; in addition, this species is currently endangered, with collection therefore restricted to its region of origin. Thus, tissue culture becomes a viable alternative for the formation of new plants. This study aimed to evaluate the in vitro induction of callus in Syngonanthus mucugensis using different BAP concentrations. The experimental design was completely randomized, with each treatment containing 4 repetitions and each repetition formed by 4 explants. The explants used were seedlings cultivated in vitro and their nodal segments. The culture medium used was MS (half-strength concentrations) supplemented with 0.0; 0.89; 1.78; 3.55; 7.10; 14.21 and 28.42 µM of BAP. At 60 days, the survival of the explants and the percentage of formation of callus were evaluated. It was verified that seedlings and nodal segments of Syngonanthus mucugensis are responsive explants to the formation of friable callus, with significant production obtained using the 1.78 and 3.55 µM BAP concentrations.A sempre-viva-de-mucugê [Syngonanthus mucugensis – Eriocaulaceae] é uma planta com grande valor ornamental e caracteriza-se pela durabilidade de suas inflorescências que se mantém mesmo depois de coletadas e secas. A propagação sexuada dessa espécie resulta em plantas desuniformes por ser geneticamente segregante e, aliada a este fato, existe a ameaça de extinção da espécie, sendo por isso restrita a coleta na sua região de origem. Desta forma, a cultura de tecidos torna-se uma alternativa viável para a formação de novas mudas e, por isso, objetivou-se, neste trabalho, avaliar a indução de calos in vitro de Syngonanthus mucugensis, utilizando diferentes concentrações de BAP. O delineamento experimental foi inteiramente casualizado (DIC), sendo que cada tratamento continha quatro repetições e cada repetição era formada por quatro explantes. Os explantes utilizados foram plantas cultivadas in vitro e segmentos nodais delas. O meio de cultivo utilizado foi o MS (metade dos sais), suplementado com (0,0; 0,89; 1,78; 3,55; 7,10; 14,21 e 28,42 µM) de BAP. Aos 60 dias, avaliaram-se a sobrevivência dos explantes e a porcentagem de formação de calos. Verificou-se que plantas inteiras e segmentos nodais de Syngonanthus mucugensis são explantes responsíveis à formação de calos friáveis, sendo que a produção significativa é obtida utilizando-se as concentrações de 1,78 e 3,55 µM de BAP.Universidade Estadual De Maringá2008-05-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/362410.4025/actascibiolsci.v30i2.3624Acta Scientiarum. Biological Sciences; Vol 30 No 2 (2008); 127-131Acta Scientiarum. Biological Sciences; v. 30 n. 2 (2008); 127-1311807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3624/2531Santos, Janilza da PaixãoDornelles, Ana Lúcia Cunha dePereira, Flávia DionísioOliveira, Lenaldo Munizinfo:eu-repo/semantics/openAccess2022-11-23T17:33:36Zoai:periodicos.uem.br/ojs:article/3624Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:36Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624
Indução de calos em sempre-viva (Syngonanthus mucugensis Giulietti), utilizando diferentes tipos de explantes e concentrações de BAP - DOI: 10.4025/actascibiolsci.v30i2.3624
title Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624
spellingShingle Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624
Santos, Janilza da Paixão
Syngonanthus mucugensis
Eriocaulaceae
cultura de tecidos
regulador de crescimento
planta ornamental
2.03.00.00-0 Botânica
title_short Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624
title_full Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624
title_fullStr Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624
title_full_unstemmed Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624
title_sort Callus induction in sempre-viva (Syngonanthus mucugensis Giulietti) using different types of explants and BAP concentrations - DOI: 10.4025/actascibiolsci.v30i3.3624
author Santos, Janilza da Paixão
author_facet Santos, Janilza da Paixão
Dornelles, Ana Lúcia Cunha de
Pereira, Flávia Dionísio
Oliveira, Lenaldo Muniz
author_role author
author2 Dornelles, Ana Lúcia Cunha de
Pereira, Flávia Dionísio
Oliveira, Lenaldo Muniz
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos, Janilza da Paixão
Dornelles, Ana Lúcia Cunha de
Pereira, Flávia Dionísio
Oliveira, Lenaldo Muniz
dc.subject.por.fl_str_mv Syngonanthus mucugensis
Eriocaulaceae
cultura de tecidos
regulador de crescimento
planta ornamental
2.03.00.00-0 Botânica
topic Syngonanthus mucugensis
Eriocaulaceae
cultura de tecidos
regulador de crescimento
planta ornamental
2.03.00.00-0 Botânica
description Sempre-viva-de-mucugê [Syngonanthus mucugensis – Eriocaulaceae] is a plant with great ornamental value characterized by the durability of its inflorescences, which remains even after collected and dried. The sexual propagation of this species results in disuniform plants, as it is genetically segregating; in addition, this species is currently endangered, with collection therefore restricted to its region of origin. Thus, tissue culture becomes a viable alternative for the formation of new plants. This study aimed to evaluate the in vitro induction of callus in Syngonanthus mucugensis using different BAP concentrations. The experimental design was completely randomized, with each treatment containing 4 repetitions and each repetition formed by 4 explants. The explants used were seedlings cultivated in vitro and their nodal segments. The culture medium used was MS (half-strength concentrations) supplemented with 0.0; 0.89; 1.78; 3.55; 7.10; 14.21 and 28.42 µM of BAP. At 60 days, the survival of the explants and the percentage of formation of callus were evaluated. It was verified that seedlings and nodal segments of Syngonanthus mucugensis are responsive explants to the formation of friable callus, with significant production obtained using the 1.78 and 3.55 µM BAP concentrations.
publishDate 2008
dc.date.none.fl_str_mv 2008-05-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3624
10.4025/actascibiolsci.v30i2.3624
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3624
identifier_str_mv 10.4025/actascibiolsci.v30i2.3624
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3624/2531
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 30 No 2 (2008); 127-131
Acta Scientiarum. Biological Sciences; v. 30 n. 2 (2008); 127-131
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
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reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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