Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101 |
Resumo: | In this study, two hundred fifty-seven bacterial isolates from a suppressive soil library were screened to study their secretion of alkali-thermostable xylanases for potential use in cellulose pulp biobleaching. Xylanase activity was evaluated in solid and liquid media using xylan as the carbon source. Isolates were initially evaluated for the degradation of xylan in solid media by the congo red test. Selected strains were evaluated in liquid media for enzymatic activity and determination of total protein concentration using a crude protein extract (CPE). An isolate identified as Bacillus species TC-DT13 produced the highest amount of xylanase (1808 U mL-1). The isolate was active and stable at 70°C and pH 9.0, conditions which are necessary for the paper industry. This isolate can grow and produce xylanase in medium containing wheat fiber as a substrate. The CPE of this isolate was used in preliminary testing on cellulose pulp bleaching; enzyme treatment of the pulp resulted in a 5% increase of whiteness. |
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Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleachingalkali–thermostable enzymesoil bacteriabiochemical characterizationBacillus spp.EnzimologiaIn this study, two hundred fifty-seven bacterial isolates from a suppressive soil library were screened to study their secretion of alkali-thermostable xylanases for potential use in cellulose pulp biobleaching. Xylanase activity was evaluated in solid and liquid media using xylan as the carbon source. Isolates were initially evaluated for the degradation of xylan in solid media by the congo red test. Selected strains were evaluated in liquid media for enzymatic activity and determination of total protein concentration using a crude protein extract (CPE). An isolate identified as Bacillus species TC-DT13 produced the highest amount of xylanase (1808 U mL-1). The isolate was active and stable at 70°C and pH 9.0, conditions which are necessary for the paper industry. This isolate can grow and produce xylanase in medium containing wheat fiber as a substrate. The CPE of this isolate was used in preliminary testing on cellulose pulp bleaching; enzyme treatment of the pulp resulted in a 5% increase of whiteness.Universidade Estadual De Maringá2019-07-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4210110.4025/actascibiolsci.v41i1.42101Acta Scientiarum. Biological Sciences; Vol 41 (2019): Publicação contínua; e42101Acta Scientiarum. Biological Sciences; v. 41 (2019): Publicação contínua; e421011807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/751375147021http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/751375147022http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/751375147023Copyright (c) 2019 Acta Scientiarum. Biological Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRodrigues, Isabela da Silva VasconcelosSilva, Carolina Goes Silva eSilva, Rafael Salomão daDolabella, Silvio SantanaFernandes, Marcelo FerreiraFernandes, Roberta Pereira Miranda2022-02-20T21:59:31Zoai:periodicos.uem.br/ojs:article/42101Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-20T21:59:31Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching |
title |
Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching |
spellingShingle |
Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching Rodrigues, Isabela da Silva Vasconcelos alkali–thermostable enzyme soil bacteria biochemical characterization Bacillus spp. Enzimologia |
title_short |
Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching |
title_full |
Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching |
title_fullStr |
Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching |
title_full_unstemmed |
Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching |
title_sort |
Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching |
author |
Rodrigues, Isabela da Silva Vasconcelos |
author_facet |
Rodrigues, Isabela da Silva Vasconcelos Silva, Carolina Goes Silva e Silva, Rafael Salomão da Dolabella, Silvio Santana Fernandes, Marcelo Ferreira Fernandes, Roberta Pereira Miranda |
author_role |
author |
author2 |
Silva, Carolina Goes Silva e Silva, Rafael Salomão da Dolabella, Silvio Santana Fernandes, Marcelo Ferreira Fernandes, Roberta Pereira Miranda |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Isabela da Silva Vasconcelos Silva, Carolina Goes Silva e Silva, Rafael Salomão da Dolabella, Silvio Santana Fernandes, Marcelo Ferreira Fernandes, Roberta Pereira Miranda |
dc.subject.por.fl_str_mv |
alkali–thermostable enzyme soil bacteria biochemical characterization Bacillus spp. Enzimologia |
topic |
alkali–thermostable enzyme soil bacteria biochemical characterization Bacillus spp. Enzimologia |
description |
In this study, two hundred fifty-seven bacterial isolates from a suppressive soil library were screened to study their secretion of alkali-thermostable xylanases for potential use in cellulose pulp biobleaching. Xylanase activity was evaluated in solid and liquid media using xylan as the carbon source. Isolates were initially evaluated for the degradation of xylan in solid media by the congo red test. Selected strains were evaluated in liquid media for enzymatic activity and determination of total protein concentration using a crude protein extract (CPE). An isolate identified as Bacillus species TC-DT13 produced the highest amount of xylanase (1808 U mL-1). The isolate was active and stable at 70°C and pH 9.0, conditions which are necessary for the paper industry. This isolate can grow and produce xylanase in medium containing wheat fiber as a substrate. The CPE of this isolate was used in preliminary testing on cellulose pulp bleaching; enzyme treatment of the pulp resulted in a 5% increase of whiteness. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101 10.4025/actascibiolsci.v41i1.42101 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101 |
identifier_str_mv |
10.4025/actascibiolsci.v41i1.42101 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/751375147021 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/751375147022 http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42101/751375147023 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Acta Scientiarum. Biological Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Acta Scientiarum. Biological Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 41 (2019): Publicação contínua; e42101 Acta Scientiarum. Biological Sciences; v. 41 (2019): Publicação contínua; e42101 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317391024324608 |