Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil

Detalhes bibliográficos
Autor(a) principal: Vianna, Fabiana Castelani Andreotti
Data de Publicação: 2023
Outros Autores: Azevedo, Rafhael Felipin, Moreira, Daiani Rodrigues, Oliveira, Taniele Carvalho de, Barelli, Marco Antonio Aparecido, Silva, Valvenarg Pereira da, Giglioli, Adriana Aparecida Sinópolis, Renesto, Erasmo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339
Resumo: Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis.
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spelling Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, BrazilEnzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazildiagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis.Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis.Universidade Estadual De Maringá2023-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6633910.4025/actascibiolsci.v45i1.66339Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e66339Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e663391807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339/751375156948Copyright (c) 2023 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVianna, Fabiana Castelani Andreotti Azevedo, Rafhael Felipin Moreira, Daiani Rodrigues Oliveira, Taniele Carvalho de Barelli, Marco Antonio Aparecido Silva, Valvenarg Pereira daGiglioli, Adriana Aparecida Sinópolis Renesto, Erasmo2024-03-01T16:11:52Zoai:periodicos.uem.br/ojs:article/66339Revistahttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/PUBhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2024-03-01T16:11:52Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
title Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
spellingShingle Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
Vianna, Fabiana Castelani Andreotti
diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.
diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.
title_short Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
title_full Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
title_fullStr Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
title_full_unstemmed Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
title_sort Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
author Vianna, Fabiana Castelani Andreotti
author_facet Vianna, Fabiana Castelani Andreotti
Azevedo, Rafhael Felipin
Moreira, Daiani Rodrigues
Oliveira, Taniele Carvalho de
Barelli, Marco Antonio Aparecido
Silva, Valvenarg Pereira da
Giglioli, Adriana Aparecida Sinópolis
Renesto, Erasmo
author_role author
author2 Azevedo, Rafhael Felipin
Moreira, Daiani Rodrigues
Oliveira, Taniele Carvalho de
Barelli, Marco Antonio Aparecido
Silva, Valvenarg Pereira da
Giglioli, Adriana Aparecida Sinópolis
Renesto, Erasmo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vianna, Fabiana Castelani Andreotti
Azevedo, Rafhael Felipin
Moreira, Daiani Rodrigues
Oliveira, Taniele Carvalho de
Barelli, Marco Antonio Aparecido
Silva, Valvenarg Pereira da
Giglioli, Adriana Aparecida Sinópolis
Renesto, Erasmo
dc.subject.por.fl_str_mv diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.
diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.
topic diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.
diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.
description Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339
10.4025/actascibiolsci.v45i1.66339
url https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339
identifier_str_mv 10.4025/actascibiolsci.v45i1.66339
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339/751375156948
dc.rights.driver.fl_str_mv Copyright (c) 2023 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e66339
Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e66339
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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