Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339 |
Resumo: | Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis. |
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Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, BrazilEnzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazildiagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis.Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis.Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis.Universidade Estadual De Maringá2023-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6633910.4025/actascibiolsci.v45i1.66339Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e66339Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e663391807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339/751375156948Copyright (c) 2023 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVianna, Fabiana Castelani Andreotti Azevedo, Rafhael Felipin Moreira, Daiani Rodrigues Oliveira, Taniele Carvalho de Barelli, Marco Antonio Aparecido Silva, Valvenarg Pereira daGiglioli, Adriana Aparecida Sinópolis Renesto, Erasmo2024-03-01T16:11:52Zoai:periodicos.uem.br/ojs:article/66339Revistahttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/PUBhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2024-03-01T16:11:52Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil |
title |
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil |
spellingShingle |
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil Vianna, Fabiana Castelani Andreotti diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis. diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis. |
title_short |
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil |
title_full |
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil |
title_fullStr |
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil |
title_full_unstemmed |
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil |
title_sort |
Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil |
author |
Vianna, Fabiana Castelani Andreotti |
author_facet |
Vianna, Fabiana Castelani Andreotti Azevedo, Rafhael Felipin Moreira, Daiani Rodrigues Oliveira, Taniele Carvalho de Barelli, Marco Antonio Aparecido Silva, Valvenarg Pereira da Giglioli, Adriana Aparecida Sinópolis Renesto, Erasmo |
author_role |
author |
author2 |
Azevedo, Rafhael Felipin Moreira, Daiani Rodrigues Oliveira, Taniele Carvalho de Barelli, Marco Antonio Aparecido Silva, Valvenarg Pereira da Giglioli, Adriana Aparecida Sinópolis Renesto, Erasmo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Vianna, Fabiana Castelani Andreotti Azevedo, Rafhael Felipin Moreira, Daiani Rodrigues Oliveira, Taniele Carvalho de Barelli, Marco Antonio Aparecido Silva, Valvenarg Pereira da Giglioli, Adriana Aparecida Sinópolis Renesto, Erasmo |
dc.subject.por.fl_str_mv |
diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis. diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis. |
topic |
diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis. diagnostic features; genetic distance; genetic variability; Paraná River basin; Pimelodella avanhandavae; Pimelodella gracilis. |
description |
Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339 10.4025/actascibiolsci.v45i1.66339 |
url |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339 |
identifier_str_mv |
10.4025/actascibiolsci.v45i1.66339 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/66339/751375156948 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e66339 Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e66339 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317390461239296 |