Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841 |
Resumo: | Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn. |
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Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841DigitariapsbA geneRAPDsequencingtriazineweedDigitariapsbA geneRAPDsequencingtriazineweedDigitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.Universidade Estadual de Maringá2010-04-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/384110.4025/actasciagron.v32i2.3841Acta Scientiarum. Agronomy; Vol 32 No 2 (2010); 255-261Acta Scientiarum. Agronomy; v. 32 n. 2 (2010); 255-2611807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841/3841http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841/3841AVieira, Viviane CristinaAlves, Pedro Luís da Costa AguiarPicchi, Simone CristinaLemos, Manoel Victor FrancoSena, Janete Apparecida Desidérioinfo:eu-repo/semantics/openAccess2022-11-23T18:38:01Zoai:periodicos.uem.br/ojs:article/3841Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:01Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 |
title |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 |
spellingShingle |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 Vieira, Viviane Cristina Digitaria psbA gene RAPD sequencing triazine weed Digitaria psbA gene RAPD sequencing triazine weed |
title_short |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 |
title_full |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 |
title_fullStr |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 |
title_full_unstemmed |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 |
title_sort |
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841 |
author |
Vieira, Viviane Cristina |
author_facet |
Vieira, Viviane Cristina Alves, Pedro Luís da Costa Aguiar Picchi, Simone Cristina Lemos, Manoel Victor Franco Sena, Janete Apparecida Desidério |
author_role |
author |
author2 |
Alves, Pedro Luís da Costa Aguiar Picchi, Simone Cristina Lemos, Manoel Victor Franco Sena, Janete Apparecida Desidério |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Viviane Cristina Alves, Pedro Luís da Costa Aguiar Picchi, Simone Cristina Lemos, Manoel Victor Franco Sena, Janete Apparecida Desidério |
dc.subject.por.fl_str_mv |
Digitaria psbA gene RAPD sequencing triazine weed Digitaria psbA gene RAPD sequencing triazine weed |
topic |
Digitaria psbA gene RAPD sequencing triazine weed Digitaria psbA gene RAPD sequencing triazine weed |
description |
Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-04-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841 10.4025/actasciagron.v32i2.3841 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841 |
identifier_str_mv |
10.4025/actasciagron.v32i2.3841 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841/3841 http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841/3841A |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 32 No 2 (2010); 255-261 Acta Scientiarum. Agronomy; v. 32 n. 2 (2010); 255-261 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305906448498688 |