Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841

Detalhes bibliográficos
Autor(a) principal: Vieira, Viviane Cristina
Data de Publicação: 2010
Outros Autores: Alves, Pedro Luís da Costa Aguiar, Picchi, Simone Cristina, Lemos, Manoel Victor Franco, Sena, Janete Apparecida Desidério
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841
Resumo: Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.
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spelling Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841DigitariapsbA geneRAPDsequencingtriazineweedDigitariapsbA geneRAPDsequencingtriazineweedDigitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.Universidade Estadual de Maringá2010-04-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/384110.4025/actasciagron.v32i2.3841Acta Scientiarum. Agronomy; Vol 32 No 2 (2010); 255-261Acta Scientiarum. Agronomy; v. 32 n. 2 (2010); 255-2611807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841/3841http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841/3841AVieira, Viviane CristinaAlves, Pedro Luís da Costa AguiarPicchi, Simone CristinaLemos, Manoel Victor FrancoSena, Janete Apparecida Desidérioinfo:eu-repo/semantics/openAccess2022-11-23T18:38:01Zoai:periodicos.uem.br/ojs:article/3841Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:01Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
title Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
spellingShingle Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
Vieira, Viviane Cristina
Digitaria
psbA gene
RAPD
sequencing
triazine
weed
Digitaria
psbA gene
RAPD
sequencing
triazine
weed
title_short Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
title_full Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
title_fullStr Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
title_full_unstemmed Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
title_sort Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn - DOI: 10.4025/actasciagron.v32i2.3841
author Vieira, Viviane Cristina
author_facet Vieira, Viviane Cristina
Alves, Pedro Luís da Costa Aguiar
Picchi, Simone Cristina
Lemos, Manoel Victor Franco
Sena, Janete Apparecida Desidério
author_role author
author2 Alves, Pedro Luís da Costa Aguiar
Picchi, Simone Cristina
Lemos, Manoel Victor Franco
Sena, Janete Apparecida Desidério
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Viviane Cristina
Alves, Pedro Luís da Costa Aguiar
Picchi, Simone Cristina
Lemos, Manoel Victor Franco
Sena, Janete Apparecida Desidério
dc.subject.por.fl_str_mv Digitaria
psbA gene
RAPD
sequencing
triazine
weed
Digitaria
psbA gene
RAPD
sequencing
triazine
weed
topic Digitaria
psbA gene
RAPD
sequencing
triazine
weed
Digitaria
psbA gene
RAPD
sequencing
triazine
weed
description Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.
publishDate 2010
dc.date.none.fl_str_mv 2010-04-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841
10.4025/actasciagron.v32i2.3841
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841
identifier_str_mv 10.4025/actasciagron.v32i2.3841
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841/3841
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841/3841A
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 32 No 2 (2010); 255-261
Acta Scientiarum. Agronomy; v. 32 n. 2 (2010); 255-261
1807-8621
1679-9275
reponame:Acta Scientiarum. Agronomy (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum. Agronomy (Online)
collection Acta Scientiarum. Agronomy (Online)
repository.name.fl_str_mv Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br
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