Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn

Detalhes bibliográficos
Autor(a) principal: Vieira, Viviane Cristina [UNESP]
Data de Publicação: 2010
Outros Autores: Alves, Pedro Luís da Costa Aguiar [UNESP], Picchi, Simone Cristina [UNESP], Lemos, Manoel Victor Franco [UNESP], Sena, Janete Apparecida Desidério [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4025/actasciagron.v32i2.3841
http://hdl.handle.net/11449/110272
Resumo: Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psbA gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.
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spelling Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametrynCaracterização molecular de acessos de capim-colchão (Digitaria nuda) e resposta à ametrinaDigitariapsbA geneRAPDSequencingTriazineWeedDigitariaGene psbARAPDSequenciamentotriazinaPlanta daninhaDigitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psbA gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.As espécies de Digitaria são plantas daninhas nas lavouras de cana-de-açúcar no Brasil e estão sendo controladas com herbicidas, porém há alguns relatos de falha no controle, notavelmente ao grupo das triazinas. Técnicas moleculares são recomendadas para análise da variabilidade genética em plantas daninhas, como o RAPD (DNA Polimórfico Amplificado ao Acaso) e PCR-RFLP (Reação da Polimerase em Cadeia - Polimorfismo no Comprimento de Fragmentos de Restrição) e, aliadas ao sequenciamento, permitem a localização de genes de resistência, bem como possíveis mutações relacionadas ao surgimento de indivíduos resistentes em algumas espécies. Com isso, o objetivo deste trabalho foi caracterizar dez acessos de Digitaria spp. por meio de marcadores RAPD e PCR-RFLP, sequenciar uma região conservada do gene psbA e analisar a resposta destes à ametrina. Pela análise molecular houve elevada similaridade genética entre os acessos, pois todos apresentaram perfis genéticos semelhantes e foram suscetíveis à ametrina.Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia Aplicada à AgropecuáriaUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e Veterinárias Departamento de Biologia Aplicada à AgropecuáriaEditora da Universidade Estadual de Maringá (EDUEM)Universidade Estadual Paulista (Unesp)Vieira, Viviane Cristina [UNESP]Alves, Pedro Luís da Costa Aguiar [UNESP]Picchi, Simone Cristina [UNESP]Lemos, Manoel Victor Franco [UNESP]Sena, Janete Apparecida Desidério [UNESP]2014-10-01T13:08:58Z2014-10-01T13:08:58Z2010-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article255-261application/pdfhttp://dx.doi.org/10.4025/actasciagron.v32i2.3841Acta Scientiarum. Agronomy. Editora da Universidade Estadual de Maringá - EDUEM, v. 32, n. 2, p. 255-261, 2010.1807-8621http://hdl.handle.net/11449/11027210.4025/actasciagron.v32i2.3841S1807-86212010000200011WOS:000279508300011S1807-86212010000200011.pdf7179273060624761129505134059183601033835242882120000-0003-2348-2121SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiarum: Agronomy0.692info:eu-repo/semantics/openAccess2023-10-30T06:11:58Zoai:repositorio.unesp.br:11449/110272Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-30T06:11:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn
Caracterização molecular de acessos de capim-colchão (Digitaria nuda) e resposta à ametrina
title Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn
spellingShingle Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn
Vieira, Viviane Cristina [UNESP]
Digitaria
psbA gene
RAPD
Sequencing
Triazine
Weed
Digitaria
Gene psbA
RAPD
Sequenciamento
triazina
Planta daninha
title_short Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn
title_full Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn
title_fullStr Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn
title_full_unstemmed Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn
title_sort Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn
author Vieira, Viviane Cristina [UNESP]
author_facet Vieira, Viviane Cristina [UNESP]
Alves, Pedro Luís da Costa Aguiar [UNESP]
Picchi, Simone Cristina [UNESP]
Lemos, Manoel Victor Franco [UNESP]
Sena, Janete Apparecida Desidério [UNESP]
author_role author
author2 Alves, Pedro Luís da Costa Aguiar [UNESP]
Picchi, Simone Cristina [UNESP]
Lemos, Manoel Victor Franco [UNESP]
Sena, Janete Apparecida Desidério [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Vieira, Viviane Cristina [UNESP]
Alves, Pedro Luís da Costa Aguiar [UNESP]
Picchi, Simone Cristina [UNESP]
Lemos, Manoel Victor Franco [UNESP]
Sena, Janete Apparecida Desidério [UNESP]
dc.subject.por.fl_str_mv Digitaria
psbA gene
RAPD
Sequencing
Triazine
Weed
Digitaria
Gene psbA
RAPD
Sequenciamento
triazina
Planta daninha
topic Digitaria
psbA gene
RAPD
Sequencing
Triazine
Weed
Digitaria
Gene psbA
RAPD
Sequenciamento
triazina
Planta daninha
description Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psbA gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
2014-10-01T13:08:58Z
2014-10-01T13:08:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4025/actasciagron.v32i2.3841
Acta Scientiarum. Agronomy. Editora da Universidade Estadual de Maringá - EDUEM, v. 32, n. 2, p. 255-261, 2010.
1807-8621
http://hdl.handle.net/11449/110272
10.4025/actasciagron.v32i2.3841
S1807-86212010000200011
WOS:000279508300011
S1807-86212010000200011.pdf
7179273060624761
1295051340591836
0103383524288212
0000-0003-2348-2121
url http://dx.doi.org/10.4025/actasciagron.v32i2.3841
http://hdl.handle.net/11449/110272
identifier_str_mv Acta Scientiarum. Agronomy. Editora da Universidade Estadual de Maringá - EDUEM, v. 32, n. 2, p. 255-261, 2010.
1807-8621
10.4025/actasciagron.v32i2.3841
S1807-86212010000200011
WOS:000279508300011
S1807-86212010000200011.pdf
7179273060624761
1295051340591836
0103383524288212
0000-0003-2348-2121
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Scientiarum: Agronomy
0.692
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 255-261
application/pdf
dc.publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá (EDUEM)
publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá (EDUEM)
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964760745181184

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