Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13872 |
Resumo: | The aim of the present work was to determine the relationship between photosynthetic pigments extracted in dimethyl sulfoxide (DMSO) and readings obtained by the portable chlorophyll meter, ClorofiLOG® 1030, to generate mathematical models that are able to report the contents of chlorophyll and carotenoids in the leaves of the castor oil plant. This work was conducted at Embrapa Cotton, located in the city of Campina Grande, state of Paraíba, Brazil, in October 2010. For the indirect analysis, portable equipment was used to read leaf discs of different shades of green. The chlorophyll in these discs was then determined using a classical method: 5 mL of DMSO was added, the samples were incubated in a water bath at 70ºC for 30 minutes, and a 3 mL aliquot was removed for spectrophotometric measurements at 470, 646 and 663 nm. The data were subjected to analysis of variance and regression analyses in which the readings obtained using the portable chlorophyll meter were the dependent variable and the photosynthetic pigments that were determined by the classical method were the independent variable. The results indicated that, with the exception of chlorophyll b, the ClorofiLOG® 1030 portable chlorophyll meter estimated the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents that are typically used in conventional procedures. |
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Acta Scientiarum. Agronomy (Online) |
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Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872chlorophyll metercalibrationchlorophyll5.01.03.00-8The aim of the present work was to determine the relationship between photosynthetic pigments extracted in dimethyl sulfoxide (DMSO) and readings obtained by the portable chlorophyll meter, ClorofiLOG® 1030, to generate mathematical models that are able to report the contents of chlorophyll and carotenoids in the leaves of the castor oil plant. This work was conducted at Embrapa Cotton, located in the city of Campina Grande, state of Paraíba, Brazil, in October 2010. For the indirect analysis, portable equipment was used to read leaf discs of different shades of green. The chlorophyll in these discs was then determined using a classical method: 5 mL of DMSO was added, the samples were incubated in a water bath at 70ºC for 30 minutes, and a 3 mL aliquot was removed for spectrophotometric measurements at 470, 646 and 663 nm. The data were subjected to analysis of variance and regression analyses in which the readings obtained using the portable chlorophyll meter were the dependent variable and the photosynthetic pigments that were determined by the classical method were the independent variable. The results indicated that, with the exception of chlorophyll b, the ClorofiLOG® 1030 portable chlorophyll meter estimated the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents that are typically used in conventional procedures.Universidade Estadual de Maringá2011-12-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1387210.4025/actasciagron.v34i3.13872Acta Scientiarum. Agronomy; Vol 34 No 3 (2012); 325-329Acta Scientiarum. Agronomy; v. 34 n. 3 (2012); 325-3291807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13872/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13872/pdf_1Rigon, João Paulo GonsiorkiewiczCapuani, SilviaBeltrão, Napoleão de Esberard MacêdoBrito Neto, José FélixSofiatti, ValdineiFrança, Fabíola Vanessainfo:eu-repo/semantics/openAccess2022-11-23T18:38:31Zoai:periodicos.uem.br/ojs:article/13872Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:31Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872 |
title |
Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872 |
spellingShingle |
Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872 Rigon, João Paulo Gonsiorkiewicz chlorophyll meter calibration chlorophyll 5.01.03.00-8 |
title_short |
Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872 |
title_full |
Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872 |
title_fullStr |
Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872 |
title_full_unstemmed |
Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872 |
title_sort |
Non-destructive determination of photosynthetic pigments in the leaves of castor oil plants - doi: 10.4025/actasciagron.v34i3.13872 |
author |
Rigon, João Paulo Gonsiorkiewicz |
author_facet |
Rigon, João Paulo Gonsiorkiewicz Capuani, Silvia Beltrão, Napoleão de Esberard Macêdo Brito Neto, José Félix Sofiatti, Valdinei França, Fabíola Vanessa |
author_role |
author |
author2 |
Capuani, Silvia Beltrão, Napoleão de Esberard Macêdo Brito Neto, José Félix Sofiatti, Valdinei França, Fabíola Vanessa |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rigon, João Paulo Gonsiorkiewicz Capuani, Silvia Beltrão, Napoleão de Esberard Macêdo Brito Neto, José Félix Sofiatti, Valdinei França, Fabíola Vanessa |
dc.subject.por.fl_str_mv |
chlorophyll meter calibration chlorophyll 5.01.03.00-8 |
topic |
chlorophyll meter calibration chlorophyll 5.01.03.00-8 |
description |
The aim of the present work was to determine the relationship between photosynthetic pigments extracted in dimethyl sulfoxide (DMSO) and readings obtained by the portable chlorophyll meter, ClorofiLOG® 1030, to generate mathematical models that are able to report the contents of chlorophyll and carotenoids in the leaves of the castor oil plant. This work was conducted at Embrapa Cotton, located in the city of Campina Grande, state of Paraíba, Brazil, in October 2010. For the indirect analysis, portable equipment was used to read leaf discs of different shades of green. The chlorophyll in these discs was then determined using a classical method: 5 mL of DMSO was added, the samples were incubated in a water bath at 70ºC for 30 minutes, and a 3 mL aliquot was removed for spectrophotometric measurements at 470, 646 and 663 nm. The data were subjected to analysis of variance and regression analyses in which the readings obtained using the portable chlorophyll meter were the dependent variable and the photosynthetic pigments that were determined by the classical method were the independent variable. The results indicated that, with the exception of chlorophyll b, the ClorofiLOG® 1030 portable chlorophyll meter estimated the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents that are typically used in conventional procedures. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13872 10.4025/actasciagron.v34i3.13872 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13872 |
identifier_str_mv |
10.4025/actasciagron.v34i3.13872 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13872/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13872/pdf_1 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 34 No 3 (2012); 325-329 Acta Scientiarum. Agronomy; v. 34 n. 3 (2012); 325-329 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305908258340864 |