Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas

Detalhes bibliográficos
Autor(a) principal: Rigon, João Paulo Gonsiorkiewicz [UNESP]
Data de Publicação: 2013
Outros Autores: Capuani, Silvia [UNESP], De Brito Neto, José Félix, De Macêdo Beltrão, Napoleão Esberard
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.5433/1679-0359.2013v34n2p669
http://hdl.handle.net/11449/74769
Resumo: The aim of this work was to generate mathematical models capable of identifying photosynthetic pigments and soluble proteins from the leaves of Jatropha curcas using the relationship between classical readings performed by spectrophotometry and the chlorophyll meter, ClorofiLOG ® 1030. The work was conducted at Embrapa Cotton, in the city of Campina Grande, state of Paraíba, Brazil. For indirect analysis, portable equipment was used to read leaf discs at different stages of development. The chlorophyll in these discs was then determined using a classical method, while the Bradford method was used to determine soluble proteins. The data were subjected to analysis of variance and regression analyses, in which the readings obtained using the portable chlorophyll meter were the dependent variables and the photosynthetic pigments and soluble protein determined by the classical method the independents variables. The results indicated that with the exception of chlorophyll b and soluble protein, the mathematical models obtained with the portable chlorophyll ClorofiLOG ® 1030 can be used to estimate the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents required for conventional procedures.
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spelling Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcasCalibrationChlorophyllChlorophyll meterSoluble proteinThe aim of this work was to generate mathematical models capable of identifying photosynthetic pigments and soluble proteins from the leaves of Jatropha curcas using the relationship between classical readings performed by spectrophotometry and the chlorophyll meter, ClorofiLOG ® 1030. The work was conducted at Embrapa Cotton, in the city of Campina Grande, state of Paraíba, Brazil. For indirect analysis, portable equipment was used to read leaf discs at different stages of development. The chlorophyll in these discs was then determined using a classical method, while the Bradford method was used to determine soluble proteins. The data were subjected to analysis of variance and regression analyses, in which the readings obtained using the portable chlorophyll meter were the dependent variables and the photosynthetic pigments and soluble protein determined by the classical method the independents variables. The results indicated that with the exception of chlorophyll b and soluble protein, the mathematical models obtained with the portable chlorophyll ClorofiLOG ® 1030 can be used to estimate the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents required for conventional procedures.Programa de Pós Graduação em Agricultura Faculdade de Ciências Agronômicas FCA/UNESP, Botucatu SPEmpresa Brasileira de Pesquisa Agropecuária EMBRAPA Centro Nacional de Pesquisa de Algodão, Centenário, Campina Grande, PBPrograma de Pós Graduação em Agricultura Faculdade de Ciências Agronômicas FCA/UNESP, Botucatu SPUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Rigon, João Paulo Gonsiorkiewicz [UNESP]Capuani, Silvia [UNESP]De Brito Neto, José FélixDe Macêdo Beltrão, Napoleão Esberard2014-05-27T11:28:37Z2014-05-27T11:28:37Z2013-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article669-674application/pdfhttp://dx.doi.org/10.5433/1679-0359.2013v34n2p669Semina:Ciencias Agrarias, v. 34, n. 2, p. 669-674, 2013.1676-546X1679-0359http://hdl.handle.net/11449/7476910.5433/1679-0359.2013v34n2p669WOS:0003282667000192-s2.0-848783431232-s2.0-84878343123.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina: Ciências Agrárias0.3490,320info:eu-repo/semantics/openAccess2024-04-09T15:37:02Zoai:repositorio.unesp.br:11449/74769Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:36:56.644708Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
title Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
spellingShingle Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
Rigon, João Paulo Gonsiorkiewicz [UNESP]
Calibration
Chlorophyll
Chlorophyll meter
Soluble protein
title_short Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
title_full Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
title_fullStr Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
title_full_unstemmed Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
title_sort Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
author Rigon, João Paulo Gonsiorkiewicz [UNESP]
author_facet Rigon, João Paulo Gonsiorkiewicz [UNESP]
Capuani, Silvia [UNESP]
De Brito Neto, José Félix
De Macêdo Beltrão, Napoleão Esberard
author_role author
author2 Capuani, Silvia [UNESP]
De Brito Neto, José Félix
De Macêdo Beltrão, Napoleão Esberard
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Rigon, João Paulo Gonsiorkiewicz [UNESP]
Capuani, Silvia [UNESP]
De Brito Neto, José Félix
De Macêdo Beltrão, Napoleão Esberard
dc.subject.por.fl_str_mv Calibration
Chlorophyll
Chlorophyll meter
Soluble protein
topic Calibration
Chlorophyll
Chlorophyll meter
Soluble protein
description The aim of this work was to generate mathematical models capable of identifying photosynthetic pigments and soluble proteins from the leaves of Jatropha curcas using the relationship between classical readings performed by spectrophotometry and the chlorophyll meter, ClorofiLOG ® 1030. The work was conducted at Embrapa Cotton, in the city of Campina Grande, state of Paraíba, Brazil. For indirect analysis, portable equipment was used to read leaf discs at different stages of development. The chlorophyll in these discs was then determined using a classical method, while the Bradford method was used to determine soluble proteins. The data were subjected to analysis of variance and regression analyses, in which the readings obtained using the portable chlorophyll meter were the dependent variables and the photosynthetic pigments and soluble protein determined by the classical method the independents variables. The results indicated that with the exception of chlorophyll b and soluble protein, the mathematical models obtained with the portable chlorophyll ClorofiLOG ® 1030 can be used to estimate the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents required for conventional procedures.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-01
2014-05-27T11:28:37Z
2014-05-27T11:28:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5433/1679-0359.2013v34n2p669
Semina:Ciencias Agrarias, v. 34, n. 2, p. 669-674, 2013.
1676-546X
1679-0359
http://hdl.handle.net/11449/74769
10.5433/1679-0359.2013v34n2p669
WOS:000328266700019
2-s2.0-84878343123
2-s2.0-84878343123.pdf
url http://dx.doi.org/10.5433/1679-0359.2013v34n2p669
http://hdl.handle.net/11449/74769
identifier_str_mv Semina:Ciencias Agrarias, v. 34, n. 2, p. 669-674, 2013.
1676-546X
1679-0359
10.5433/1679-0359.2013v34n2p669
WOS:000328266700019
2-s2.0-84878343123
2-s2.0-84878343123.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Semina: Ciências Agrárias
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0,320
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 669-674
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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