Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5433/1679-0359.2013v34n2p669 http://hdl.handle.net/11449/74769 |
Resumo: | The aim of this work was to generate mathematical models capable of identifying photosynthetic pigments and soluble proteins from the leaves of Jatropha curcas using the relationship between classical readings performed by spectrophotometry and the chlorophyll meter, ClorofiLOG ® 1030. The work was conducted at Embrapa Cotton, in the city of Campina Grande, state of Paraíba, Brazil. For indirect analysis, portable equipment was used to read leaf discs at different stages of development. The chlorophyll in these discs was then determined using a classical method, while the Bradford method was used to determine soluble proteins. The data were subjected to analysis of variance and regression analyses, in which the readings obtained using the portable chlorophyll meter were the dependent variables and the photosynthetic pigments and soluble protein determined by the classical method the independents variables. The results indicated that with the exception of chlorophyll b and soluble protein, the mathematical models obtained with the portable chlorophyll ClorofiLOG ® 1030 can be used to estimate the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents required for conventional procedures. |
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Repositório Institucional da UNESP |
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Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcasCalibrationChlorophyllChlorophyll meterSoluble proteinThe aim of this work was to generate mathematical models capable of identifying photosynthetic pigments and soluble proteins from the leaves of Jatropha curcas using the relationship between classical readings performed by spectrophotometry and the chlorophyll meter, ClorofiLOG ® 1030. The work was conducted at Embrapa Cotton, in the city of Campina Grande, state of Paraíba, Brazil. For indirect analysis, portable equipment was used to read leaf discs at different stages of development. The chlorophyll in these discs was then determined using a classical method, while the Bradford method was used to determine soluble proteins. The data were subjected to analysis of variance and regression analyses, in which the readings obtained using the portable chlorophyll meter were the dependent variables and the photosynthetic pigments and soluble protein determined by the classical method the independents variables. The results indicated that with the exception of chlorophyll b and soluble protein, the mathematical models obtained with the portable chlorophyll ClorofiLOG ® 1030 can be used to estimate the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents required for conventional procedures.Programa de Pós Graduação em Agricultura Faculdade de Ciências Agronômicas FCA/UNESP, Botucatu SPEmpresa Brasileira de Pesquisa Agropecuária EMBRAPA Centro Nacional de Pesquisa de Algodão, Centenário, Campina Grande, PBPrograma de Pós Graduação em Agricultura Faculdade de Ciências Agronômicas FCA/UNESP, Botucatu SPUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Rigon, João Paulo Gonsiorkiewicz [UNESP]Capuani, Silvia [UNESP]De Brito Neto, José FélixDe Macêdo Beltrão, Napoleão Esberard2014-05-27T11:28:37Z2014-05-27T11:28:37Z2013-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article669-674application/pdfhttp://dx.doi.org/10.5433/1679-0359.2013v34n2p669Semina:Ciencias Agrarias, v. 34, n. 2, p. 669-674, 2013.1676-546X1679-0359http://hdl.handle.net/11449/7476910.5433/1679-0359.2013v34n2p669WOS:0003282667000192-s2.0-848783431232-s2.0-84878343123.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSemina: Ciências Agrárias0.3490,320info:eu-repo/semantics/openAccess2024-04-09T15:37:02Zoai:repositorio.unesp.br:11449/74769Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:36:56.644708Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas |
title |
Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas |
spellingShingle |
Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas Rigon, João Paulo Gonsiorkiewicz [UNESP] Calibration Chlorophyll Chlorophyll meter Soluble protein |
title_short |
Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas |
title_full |
Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas |
title_fullStr |
Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas |
title_full_unstemmed |
Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas |
title_sort |
Indirect measurement of photosynthetic pigments in the leaves of Jatropha curcas |
author |
Rigon, João Paulo Gonsiorkiewicz [UNESP] |
author_facet |
Rigon, João Paulo Gonsiorkiewicz [UNESP] Capuani, Silvia [UNESP] De Brito Neto, José Félix De Macêdo Beltrão, Napoleão Esberard |
author_role |
author |
author2 |
Capuani, Silvia [UNESP] De Brito Neto, José Félix De Macêdo Beltrão, Napoleão Esberard |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Rigon, João Paulo Gonsiorkiewicz [UNESP] Capuani, Silvia [UNESP] De Brito Neto, José Félix De Macêdo Beltrão, Napoleão Esberard |
dc.subject.por.fl_str_mv |
Calibration Chlorophyll Chlorophyll meter Soluble protein |
topic |
Calibration Chlorophyll Chlorophyll meter Soluble protein |
description |
The aim of this work was to generate mathematical models capable of identifying photosynthetic pigments and soluble proteins from the leaves of Jatropha curcas using the relationship between classical readings performed by spectrophotometry and the chlorophyll meter, ClorofiLOG ® 1030. The work was conducted at Embrapa Cotton, in the city of Campina Grande, state of Paraíba, Brazil. For indirect analysis, portable equipment was used to read leaf discs at different stages of development. The chlorophyll in these discs was then determined using a classical method, while the Bradford method was used to determine soluble proteins. The data were subjected to analysis of variance and regression analyses, in which the readings obtained using the portable chlorophyll meter were the dependent variables and the photosynthetic pigments and soluble protein determined by the classical method the independents variables. The results indicated that with the exception of chlorophyll b and soluble protein, the mathematical models obtained with the portable chlorophyll ClorofiLOG ® 1030 can be used to estimate the concentration of photosynthetic pigments with high precision, thus saving time and the chemical reagents required for conventional procedures. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 2014-05-27T11:28:37Z 2014-05-27T11:28:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5433/1679-0359.2013v34n2p669 Semina:Ciencias Agrarias, v. 34, n. 2, p. 669-674, 2013. 1676-546X 1679-0359 http://hdl.handle.net/11449/74769 10.5433/1679-0359.2013v34n2p669 WOS:000328266700019 2-s2.0-84878343123 2-s2.0-84878343123.pdf |
url |
http://dx.doi.org/10.5433/1679-0359.2013v34n2p669 http://hdl.handle.net/11449/74769 |
identifier_str_mv |
Semina:Ciencias Agrarias, v. 34, n. 2, p. 669-674, 2013. 1676-546X 1679-0359 10.5433/1679-0359.2013v34n2p669 WOS:000328266700019 2-s2.0-84878343123 2-s2.0-84878343123.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Semina: Ciências Agrárias 0.349 0,320 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
669-674 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129227601477632 |