Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35248 |
Resumo: | Gliricidia (Gliricidia sepium) and sombreiro (Clitoria fairchildiana) have been recommended for agroforestry systems and reforestation of degraded areas due to their fast growth and symbiosis with rhizobia. However, little is known about native populations that nodulate these species. The objective of this study was to evaluate the phenotypic and genetic diversity of nitrogen-fixing bacteria isolated from nodules of gliricidia and sombreiro in alley cropping systems located in the pre-Amazon region of Maranhão State and to confirm their nodulation ability. Nodules were field collected from 20 plants of each species. The isolated strains were characterized morphologically, their 16S rRNA gene was partially sequenced, and their symbiotic ability was authenticated in siratro (Macroptilium atropurpureum). Despite being in the same climate and soil conditions, gliricidia and sombreiro are nodulated by different rhizobia genera, with Rhizobium predominant in gliricidia and Bradyrhizobium in sombreiro. Endophytic strains also colonized nodules in the field. Approximately 60% of Rhizobium strains did not nodulate siratro, whereas all Bradyrhizobium strains did. Native strains isolated from gliricidia nodules had low efficiency, and only four strains isolated from sombreiro nodules were efficient in siratro. These results highlight the importance of symbiotic relationships in the regulation of biological nitrogen fixation. |
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Acta Scientiarum. Agronomy (Online) |
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Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão StateBradyrhizobiumRhizobiumPapilionoideaetree legumesacid soil.Microbiologia e Bioquímica do Solo Gliricidia (Gliricidia sepium) and sombreiro (Clitoria fairchildiana) have been recommended for agroforestry systems and reforestation of degraded areas due to their fast growth and symbiosis with rhizobia. However, little is known about native populations that nodulate these species. The objective of this study was to evaluate the phenotypic and genetic diversity of nitrogen-fixing bacteria isolated from nodules of gliricidia and sombreiro in alley cropping systems located in the pre-Amazon region of Maranhão State and to confirm their nodulation ability. Nodules were field collected from 20 plants of each species. The isolated strains were characterized morphologically, their 16S rRNA gene was partially sequenced, and their symbiotic ability was authenticated in siratro (Macroptilium atropurpureum). Despite being in the same climate and soil conditions, gliricidia and sombreiro are nodulated by different rhizobia genera, with Rhizobium predominant in gliricidia and Bradyrhizobium in sombreiro. Endophytic strains also colonized nodules in the field. Approximately 60% of Rhizobium strains did not nodulate siratro, whereas all Bradyrhizobium strains did. Native strains isolated from gliricidia nodules had low efficiency, and only four strains isolated from sombreiro nodules were efficient in siratro. These results highlight the importance of symbiotic relationships in the regulation of biological nitrogen fixation. Universidade Estadual de Maringá2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3524810.4025/actasciagron.v40i1.35248Acta Scientiarum. Agronomy; Vol 40 (2018): Publicação Contínua; e35248Acta Scientiarum. Agronomy; v. 40 (2018): Publicação Contínua; e352481807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35248/pdfCopyright (c) 2018 Acta Scientiarum. Agronomyinfo:eu-repo/semantics/openAccessCoelho, Katia PereiraRibeiro, Paula Rose de AlmeidaMoura, Emanoel Gomes deAguiar, Alana das Chagas FerreiraRodrigues, Tainara LouzadaMoreira, Fatima Maria de Souza2019-09-24T12:26:47Zoai:periodicos.uem.br/ojs:article/35248Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2019-09-24T12:26:47Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State |
title |
Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State |
spellingShingle |
Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State Coelho, Katia Pereira Bradyrhizobium Rhizobium Papilionoideae tree legumes acid soil. Microbiologia e Bioquímica do Solo |
title_short |
Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State |
title_full |
Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State |
title_fullStr |
Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State |
title_full_unstemmed |
Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State |
title_sort |
Symbiosis of rhizobia with Gliricidia sepium and Clitoria fairchildiana in an Oxisol in the pre-Amazon region of Maranhão State |
author |
Coelho, Katia Pereira |
author_facet |
Coelho, Katia Pereira Ribeiro, Paula Rose de Almeida Moura, Emanoel Gomes de Aguiar, Alana das Chagas Ferreira Rodrigues, Tainara Louzada Moreira, Fatima Maria de Souza |
author_role |
author |
author2 |
Ribeiro, Paula Rose de Almeida Moura, Emanoel Gomes de Aguiar, Alana das Chagas Ferreira Rodrigues, Tainara Louzada Moreira, Fatima Maria de Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Coelho, Katia Pereira Ribeiro, Paula Rose de Almeida Moura, Emanoel Gomes de Aguiar, Alana das Chagas Ferreira Rodrigues, Tainara Louzada Moreira, Fatima Maria de Souza |
dc.subject.por.fl_str_mv |
Bradyrhizobium Rhizobium Papilionoideae tree legumes acid soil. Microbiologia e Bioquímica do Solo |
topic |
Bradyrhizobium Rhizobium Papilionoideae tree legumes acid soil. Microbiologia e Bioquímica do Solo |
description |
Gliricidia (Gliricidia sepium) and sombreiro (Clitoria fairchildiana) have been recommended for agroforestry systems and reforestation of degraded areas due to their fast growth and symbiosis with rhizobia. However, little is known about native populations that nodulate these species. The objective of this study was to evaluate the phenotypic and genetic diversity of nitrogen-fixing bacteria isolated from nodules of gliricidia and sombreiro in alley cropping systems located in the pre-Amazon region of Maranhão State and to confirm their nodulation ability. Nodules were field collected from 20 plants of each species. The isolated strains were characterized morphologically, their 16S rRNA gene was partially sequenced, and their symbiotic ability was authenticated in siratro (Macroptilium atropurpureum). Despite being in the same climate and soil conditions, gliricidia and sombreiro are nodulated by different rhizobia genera, with Rhizobium predominant in gliricidia and Bradyrhizobium in sombreiro. Endophytic strains also colonized nodules in the field. Approximately 60% of Rhizobium strains did not nodulate siratro, whereas all Bradyrhizobium strains did. Native strains isolated from gliricidia nodules had low efficiency, and only four strains isolated from sombreiro nodules were efficient in siratro. These results highlight the importance of symbiotic relationships in the regulation of biological nitrogen fixation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35248 10.4025/actasciagron.v40i1.35248 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35248 |
identifier_str_mv |
10.4025/actasciagron.v40i1.35248 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35248/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Acta Scientiarum. Agronomy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Acta Scientiarum. Agronomy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 40 (2018): Publicação Contínua; e35248 Acta Scientiarum. Agronomy; v. 40 (2018): Publicação Contínua; e35248 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
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1799305909995831296 |