Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19373 |
Resumo: | This research aimed to investigate the cellular alterations during the loss and re-establishment of desiccation tolerance (DT) in germinated Sesbania virgata seeds. The loss of DT was characterized in germinated seeds with increasing radicle lengths (1, 2, 3, 4 and 5 mm) when subjected to dehydration in silica gel, followed by rehydration. To re-establish DT, the germinated seeds were incubated for 72h in polyethylene glycol (PEG, -2.04 MPa) with or without ABA (100 μM) before dehydration in silica gel. Cell viability was assessed by seedling survival, and DNA integrity was evaluated by gel electrophoresis. Seeds with 1 mm radicle length survived dehydration to the original moisture content (MC) of the dry seed (approximately 10%). PEG treatment was able to re-establish DT, at least partially, with 2, 3 and 4 mm but not in 5 mm radicle lengths. Germinated seeds treated with PEG+ABA performed better than those treated only with PEG, and DT was re-established even in germinated seeds with a 5 mm radicle length. Among the PEG-treated germinated seeds dehydrated to 10% MC, DNA integrity was maintained only in those with a 1 mm radicle length. |
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Acta Scientiarum. Agronomy (Online) |
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Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.)abscisic acidcytological alterationosmotic stressProdução e Tecnologia de sementesThis research aimed to investigate the cellular alterations during the loss and re-establishment of desiccation tolerance (DT) in germinated Sesbania virgata seeds. The loss of DT was characterized in germinated seeds with increasing radicle lengths (1, 2, 3, 4 and 5 mm) when subjected to dehydration in silica gel, followed by rehydration. To re-establish DT, the germinated seeds were incubated for 72h in polyethylene glycol (PEG, -2.04 MPa) with or without ABA (100 μM) before dehydration in silica gel. Cell viability was assessed by seedling survival, and DNA integrity was evaluated by gel electrophoresis. Seeds with 1 mm radicle length survived dehydration to the original moisture content (MC) of the dry seed (approximately 10%). PEG treatment was able to re-establish DT, at least partially, with 2, 3 and 4 mm but not in 5 mm radicle lengths. Germinated seeds treated with PEG+ABA performed better than those treated only with PEG, and DT was re-established even in germinated seeds with a 5 mm radicle length. Among the PEG-treated germinated seeds dehydrated to 10% MC, DNA integrity was maintained only in those with a 1 mm radicle length. Universidade Estadual de Maringá2015-08-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1937310.4025/actasciagron.v37i3.19373Acta Scientiarum. Agronomy; Vol 37 No 3 (2015); 313-320Acta Scientiarum. Agronomy; v. 37 n. 3 (2015); 313-3201807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19373/pdf_81Masetto, Tathiana ElisaFaria, José Marcio RochaFraiz, Ana Carla Rezendeinfo:eu-repo/semantics/openAccess2015-08-03T17:00:18Zoai:periodicos.uem.br/ojs:article/19373Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2015-08-03T17:00:18Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) |
title |
Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) |
spellingShingle |
Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) Masetto, Tathiana Elisa abscisic acid cytological alteration osmotic stress Produção e Tecnologia de sementes |
title_short |
Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) |
title_full |
Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) |
title_fullStr |
Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) |
title_full_unstemmed |
Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) |
title_sort |
Loss and re-establishment of desiccation tolerance in the germinated seeds of Sesbania virgata (Cav.) (Pers.) |
author |
Masetto, Tathiana Elisa |
author_facet |
Masetto, Tathiana Elisa Faria, José Marcio Rocha Fraiz, Ana Carla Rezende |
author_role |
author |
author2 |
Faria, José Marcio Rocha Fraiz, Ana Carla Rezende |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Masetto, Tathiana Elisa Faria, José Marcio Rocha Fraiz, Ana Carla Rezende |
dc.subject.por.fl_str_mv |
abscisic acid cytological alteration osmotic stress Produção e Tecnologia de sementes |
topic |
abscisic acid cytological alteration osmotic stress Produção e Tecnologia de sementes |
description |
This research aimed to investigate the cellular alterations during the loss and re-establishment of desiccation tolerance (DT) in germinated Sesbania virgata seeds. The loss of DT was characterized in germinated seeds with increasing radicle lengths (1, 2, 3, 4 and 5 mm) when subjected to dehydration in silica gel, followed by rehydration. To re-establish DT, the germinated seeds were incubated for 72h in polyethylene glycol (PEG, -2.04 MPa) with or without ABA (100 μM) before dehydration in silica gel. Cell viability was assessed by seedling survival, and DNA integrity was evaluated by gel electrophoresis. Seeds with 1 mm radicle length survived dehydration to the original moisture content (MC) of the dry seed (approximately 10%). PEG treatment was able to re-establish DT, at least partially, with 2, 3 and 4 mm but not in 5 mm radicle lengths. Germinated seeds treated with PEG+ABA performed better than those treated only with PEG, and DT was re-established even in germinated seeds with a 5 mm radicle length. Among the PEG-treated germinated seeds dehydrated to 10% MC, DNA integrity was maintained only in those with a 1 mm radicle length. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19373 10.4025/actasciagron.v37i3.19373 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19373 |
identifier_str_mv |
10.4025/actasciagron.v37i3.19373 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19373/pdf_81 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 37 No 3 (2015); 313-320 Acta Scientiarum. Agronomy; v. 37 n. 3 (2015); 313-320 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305909300625408 |