In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315

• Autor(a) principal: Schuelter, Adilson Ricken
• Data de Publicação: 2008
• Outros Autores: Luz, Cristiana Leonardi da, Dal’Maso, Alexandre, Vieira, Elisa Serra Negra, Barreto, Rafael Reccanello
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Acta Scientiarum. Agronomy (Online)
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5315
• topic: sexual reproduction; stigma receptivity; RAPD; reprodução sexuada; receptividade do estigma; RAPD.

The type of sexual reproduction of the species amazonic named cubiu is until discussed among researchers. Thus, the present work had the goal to evaluate the control of pollination in the fruiting of cubiu, to verify the influence of the developmental stage of the floral buds and time of pollination on the stigma receptivity, and in vitro germination of the pollen grain. Anthers from the flowers grown in the greenhouse were grinded and the pollen grains were placed in Petri dishes to evaluate the influence of sucrose, time of harvest and stage of flower development on the germination. A DNA sample from young leaves was purified, amplified by the RAPD technique. The results pointed out that the environment of growth interfered in the flower formation, inflorescences and fruiting. The use of different concentrations of sucrose did not affect the number of germinated pollen grain. The stigma analyses showed that its receptivity was inde-pendent from the time of harvest and stage of flower development. The RAPD analysis showed occurrence of intra-variety genetic variability. Thus, the cubiu is alogamic plant, but based on the rate of pollen germination and stigma receptivity the plant may have occurrence of self pollination.