In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5315 |
Resumo: | The type of sexual reproduction of the species amazonic named cubiu is until discussed among researchers. Thus, the present work had the goal to evaluate the control of pollination in the fruiting of cubiu, to verify the influence of the developmental stage of the floral buds and time of pollination on the stigma receptivity, and in vitro germination of the pollen grain. Anthers from the flowers grown in the greenhouse were grinded and the pollen grains were placed in Petri dishes to evaluate the influence of sucrose, time of harvest and stage of flower development on the germination. A DNA sample from young leaves was purified, amplified by the RAPD technique. The results pointed out that the environment of growth interfered in the flower formation, inflorescences and fruiting. The use of different concentrations of sucrose did not affect the number of germinated pollen grain. The stigma analyses showed that its receptivity was inde-pendent from the time of harvest and stage of flower development. The RAPD analysis showed occurrence of intra-variety genetic variability. Thus, the cubiu is alogamic plant, but based on the rate of pollen germination and stigma receptivity the plant may have occurrence of self pollination. |
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In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315Germinação in vitro de grãos de pólen e efeito da proteção das plantas na frutificação de cubiu (Solanum sessiliflorum Dunal) - DOI: 10.4025/actasciagron.v30i4.5315sexual reproductionstigma receptivityRAPDreprodução sexuadareceptividade do estigmaRAPD.The type of sexual reproduction of the species amazonic named cubiu is until discussed among researchers. Thus, the present work had the goal to evaluate the control of pollination in the fruiting of cubiu, to verify the influence of the developmental stage of the floral buds and time of pollination on the stigma receptivity, and in vitro germination of the pollen grain. Anthers from the flowers grown in the greenhouse were grinded and the pollen grains were placed in Petri dishes to evaluate the influence of sucrose, time of harvest and stage of flower development on the germination. A DNA sample from young leaves was purified, amplified by the RAPD technique. The results pointed out that the environment of growth interfered in the flower formation, inflorescences and fruiting. The use of different concentrations of sucrose did not affect the number of germinated pollen grain. The stigma analyses showed that its receptivity was inde-pendent from the time of harvest and stage of flower development. The RAPD analysis showed occurrence of intra-variety genetic variability. Thus, the cubiu is alogamic plant, but based on the rate of pollen germination and stigma receptivity the plant may have occurrence of self pollination.O tipo de reprodução sexuada da planta cubiu é ainda discutido entre os pesquisadores. O trabalho teve como objetivos: avaliar o controle da polinização na frutificação de cubiu, verificar o efeito do estádio de desenvolvimento do botão floral e do horário na receptividade do estigma e na germinação in vitro de grãos de pólen. Anteras de flores de plantas cultivadas em casa-de-vegetação foram maceradas e os grãos de pólen inoculados em placas Petri para avaliação do efeito da concentração de sacarose, do horário de coleta e do estádio das flores na germinação in vitro. DNA de amostras de folhas jovens foram purificadas, amplificados por marcadores RAPD. Os resultados indicaram que o ambiente de cultivo interferiu na formação de flores, inflorescências e frutos. O emprego de diferentes concentrações de sacarose não promoveu modificações no número médio de grãos de pólen germinados. Pela análise dos estigmas, verificou-se que a receptividade independe dos horários de coleta e do estádio das flores. Pelas análises de marcadores RAPD, verificou-se a ocorrência de variabilidade genética intra-varietal. Assim, conclui-se que o cubiu é uma planta alógama, porém pelas taxas de germinação dos grãos de pólen e pela receptividade do estigma há indícios de ocorrência de autofecundação.Universidade Estadual de Maringá2008-10-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/531510.4025/actasciagron.v30i4.5315Acta Scientiarum. Agronomy; Vol 30 No 4 (2008); 539-545Acta Scientiarum. Agronomy; v. 30 n. 4 (2008); 539-5451807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5315/5315Schuelter, Adilson RickenLuz, Cristiana Leonardi daDal’Maso, AlexandreVieira, Elisa Serra NegraBarreto, Rafael Reccanelloinfo:eu-repo/semantics/openAccess2022-11-23T18:38:10Zoai:periodicos.uem.br/ojs:article/5315Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:10Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315 Germinação in vitro de grãos de pólen e efeito da proteção das plantas na frutificação de cubiu (Solanum sessiliflorum Dunal) - DOI: 10.4025/actasciagron.v30i4.5315 |
title |
In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315 |
spellingShingle |
In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315 Schuelter, Adilson Ricken sexual reproduction stigma receptivity RAPD reprodução sexuada receptividade do estigma RAPD. |
title_short |
In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315 |
title_full |
In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315 |
title_fullStr |
In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315 |
title_full_unstemmed |
In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315 |
title_sort |
In vitro germination of pollen and effect of plant covering in the cubiu (Solanum sessiliflorum Dunal) frutification - DOI: 10.4025/actasciagron.v30i4.5315 |
author |
Schuelter, Adilson Ricken |
author_facet |
Schuelter, Adilson Ricken Luz, Cristiana Leonardi da Dal’Maso, Alexandre Vieira, Elisa Serra Negra Barreto, Rafael Reccanello |
author_role |
author |
author2 |
Luz, Cristiana Leonardi da Dal’Maso, Alexandre Vieira, Elisa Serra Negra Barreto, Rafael Reccanello |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Schuelter, Adilson Ricken Luz, Cristiana Leonardi da Dal’Maso, Alexandre Vieira, Elisa Serra Negra Barreto, Rafael Reccanello |
dc.subject.por.fl_str_mv |
sexual reproduction stigma receptivity RAPD reprodução sexuada receptividade do estigma RAPD. |
topic |
sexual reproduction stigma receptivity RAPD reprodução sexuada receptividade do estigma RAPD. |
description |
The type of sexual reproduction of the species amazonic named cubiu is until discussed among researchers. Thus, the present work had the goal to evaluate the control of pollination in the fruiting of cubiu, to verify the influence of the developmental stage of the floral buds and time of pollination on the stigma receptivity, and in vitro germination of the pollen grain. Anthers from the flowers grown in the greenhouse were grinded and the pollen grains were placed in Petri dishes to evaluate the influence of sucrose, time of harvest and stage of flower development on the germination. A DNA sample from young leaves was purified, amplified by the RAPD technique. The results pointed out that the environment of growth interfered in the flower formation, inflorescences and fruiting. The use of different concentrations of sucrose did not affect the number of germinated pollen grain. The stigma analyses showed that its receptivity was inde-pendent from the time of harvest and stage of flower development. The RAPD analysis showed occurrence of intra-variety genetic variability. Thus, the cubiu is alogamic plant, but based on the rate of pollen germination and stigma receptivity the plant may have occurrence of self pollination. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5315 10.4025/actasciagron.v30i4.5315 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5315 |
identifier_str_mv |
10.4025/actasciagron.v30i4.5315 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5315/5315 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 30 No 4 (2008); 539-545 Acta Scientiarum. Agronomy; v. 30 n. 4 (2008); 539-545 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305907081838592 |