Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279

Detalhes bibliográficos
Autor(a) principal: Kavand, Somayeh
Data de Publicação: 2011
Outros Autores: Kermani, Maryam Jafarkhani, Haghnazari, Ali, Khosravi, Pegah, Azimi, Mohamad Reza
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Acta Scientiarum. Agronomy (Online)
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279
Resumo: In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM IBA + 0.5 µM NAA during the rooting stage. Comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron.
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spelling Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279in vitro conservationmicropropagationproliferationrecoveryrootingRosa pulverulentain vitro conservationmicropropagationproliferationrecoveryrootingRosa pulverulentaIn Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM IBA + 0.5 µM NAA during the rooting stage. Comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron.In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM IBA + 0.5 µM NAA during the rooting stage. Comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron.Universidade Estadual de Maringá2011-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLaboratory analysisapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1027910.4025/actasciagron.v33i2.10279Acta Scientiarum. Agronomy; Vol 33 No 2 (2011); 297-301Acta Scientiarum. Agronomy; v. 33 n. 2 (2011); 297-3011807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMengporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279/10279http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279/10279AKavand, SomayehKermani, Maryam JafarkhaniHaghnazari, AliKhosravi, PegahAzimi, Mohamad Rezainfo:eu-repo/semantics/openAccess2022-11-23T18:38:24Zoai:periodicos.uem.br/ojs:article/10279Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:24Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
title Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
spellingShingle Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
Kavand, Somayeh
in vitro conservation
micropropagation
proliferation
recovery
rooting
Rosa pulverulenta
in vitro conservation
micropropagation
proliferation
recovery
rooting
Rosa pulverulenta
title_short Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
title_full Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
title_fullStr Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
title_full_unstemmed Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
title_sort Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
author Kavand, Somayeh
author_facet Kavand, Somayeh
Kermani, Maryam Jafarkhani
Haghnazari, Ali
Khosravi, Pegah
Azimi, Mohamad Reza
author_role author
author2 Kermani, Maryam Jafarkhani
Haghnazari, Ali
Khosravi, Pegah
Azimi, Mohamad Reza
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Kavand, Somayeh
Kermani, Maryam Jafarkhani
Haghnazari, Ali
Khosravi, Pegah
Azimi, Mohamad Reza
dc.subject.por.fl_str_mv in vitro conservation
micropropagation
proliferation
recovery
rooting
Rosa pulverulenta
in vitro conservation
micropropagation
proliferation
recovery
rooting
Rosa pulverulenta
topic in vitro conservation
micropropagation
proliferation
recovery
rooting
Rosa pulverulenta
in vitro conservation
micropropagation
proliferation
recovery
rooting
Rosa pulverulenta
description In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM IBA + 0.5 µM NAA during the rooting stage. Comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-16
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Laboratory analysis
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dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279
10.4025/actasciagron.v33i2.10279
url http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279
identifier_str_mv 10.4025/actasciagron.v33i2.10279
dc.language.iso.fl_str_mv eng
por
language eng
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dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279/10279
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279/10279A
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
publisher.none.fl_str_mv Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Agronomy; Vol 33 No 2 (2011); 297-301
Acta Scientiarum. Agronomy; v. 33 n. 2 (2011); 297-301
1807-8621
1679-9275
reponame:Acta Scientiarum. Agronomy (Online)
instname:Universidade Estadual de Maringá (UEM)
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reponame_str Acta Scientiarum. Agronomy (Online)
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