Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279 |
Resumo: | In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM IBA + 0.5 µM NAA during the rooting stage. Comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron. |
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Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279in vitro conservationmicropropagationproliferationrecoveryrootingRosa pulverulentain vitro conservationmicropropagationproliferationrecoveryrootingRosa pulverulentaIn Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM IBA + 0.5 µM NAA during the rooting stage. Comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron.In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM IBA + 0.5 µM NAA during the rooting stage. Comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron.Universidade Estadual de Maringá2011-02-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionLaboratory analysisapplication/pdfapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1027910.4025/actasciagron.v33i2.10279Acta Scientiarum. Agronomy; Vol 33 No 2 (2011); 297-301Acta Scientiarum. Agronomy; v. 33 n. 2 (2011); 297-3011807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMengporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279/10279http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279/10279AKavand, SomayehKermani, Maryam JafarkhaniHaghnazari, AliKhosravi, PegahAzimi, Mohamad Rezainfo:eu-repo/semantics/openAccess2022-11-23T18:38:24Zoai:periodicos.uem.br/ojs:article/10279Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:24Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 |
title |
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 |
spellingShingle |
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 Kavand, Somayeh in vitro conservation micropropagation proliferation recovery rooting Rosa pulverulenta in vitro conservation micropropagation proliferation recovery rooting Rosa pulverulenta |
title_short |
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 |
title_full |
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 |
title_fullStr |
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 |
title_full_unstemmed |
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 |
title_sort |
Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 |
author |
Kavand, Somayeh |
author_facet |
Kavand, Somayeh Kermani, Maryam Jafarkhani Haghnazari, Ali Khosravi, Pegah Azimi, Mohamad Reza |
author_role |
author |
author2 |
Kermani, Maryam Jafarkhani Haghnazari, Ali Khosravi, Pegah Azimi, Mohamad Reza |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Kavand, Somayeh Kermani, Maryam Jafarkhani Haghnazari, Ali Khosravi, Pegah Azimi, Mohamad Reza |
dc.subject.por.fl_str_mv |
in vitro conservation micropropagation proliferation recovery rooting Rosa pulverulenta in vitro conservation micropropagation proliferation recovery rooting Rosa pulverulenta |
topic |
in vitro conservation micropropagation proliferation recovery rooting Rosa pulverulenta in vitro conservation micropropagation proliferation recovery rooting Rosa pulverulenta |
description |
In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM IBA + 0.5 µM NAA during the rooting stage. Comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Laboratory analysis |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279 10.4025/actasciagron.v33i2.10279 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279 |
identifier_str_mv |
10.4025/actasciagron.v33i2.10279 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279/10279 http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279/10279A |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 33 No 2 (2011); 297-301 Acta Scientiarum. Agronomy; v. 33 n. 2 (2011); 297-301 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305908158726144 |