Developmental of protocol for micrograffiting to tomato Lycopersicon esculentum Mill - DOI: 10.4025/actasciagron.v32i1.968

• Autor(a) principal: Coutinho, Ozimar de Lima
• Data de Publicação: 2009
• Outros Autores: Rego, Maílson Monteiro do, Rego, Elizanilda Ramalho do, Kitamura, Mario Couquiti, Marques, Luciano Façanha, Farias Filho, Leonildo de Paula
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Acta Scientiarum. Agronomy (Online)
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/968
• topic: Solanaceae; bacterial wilt; Ralstonia solanacearum; micropropagation; in vitro culture; Solanaceae; murcha bacteriana; Ralstonia solanacearum; micropropagação; cultura in vitro; 5.01.03.05-9

The bacterial wilt, caused by Ralstonia solanacearum, is the most important vascular disease among plants in the world. The causal agent is a strain of bacteria found in all regions of Brazil, mainly in places with high humidity and temperature. This bacterium is difficult to control with chemical products, being able to survive in diverse hosts, especially in species of Solanaceae as well as other families of great food, condimental, or medicinal importance. The use of species of the genus Solanum has been attempted, but the practice of grafting and micrografting of tomato Lycopersicon esculentum is limited by incompatibility. In this work, the micrografting method was used to obtain resistant plants using the species >em>Solanum palinacanthum Dun., which features a series of interesting characteristics from a commercial point of view, mainly the resistance to diseases that are difficult to control, as in the case of bacterial wilt. For this, two methods of micrografting were used – the conventional inverted T method; and the Bisel cut method. The experiment was submitted to five evaluations in weekly intervals. It was determined that MS salt medium in the 1/8 concentration, added to sucrose (30 g L-1), showed to be suitable for the practice of micrografting using the inverted T method.