Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Acta Scientiarum. Agronomy (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062 |
Resumo: | Research on anatomical modifications under in vitro culture is essential to the definition and understanding of the development of micropropagated plants. Likewise, such research is essential to improve the steps of the acclimatization process. Accordingly, the objective of the present study is to verify the differences in the leaves anatomical traits of micropropagated orchids under calcium silicate concentrations. Seedlings of in vitro-germinated seeds, measuring 0.5 cm in length, were inoculated in 250 cm3 pots with 60 mL MS culture medium and different silicon concentrations (0, 0.5, 1.0 and 2.0 mg L-1) in different culture environments (natural environment, in a greenhouse and an artificial environment in a growth chamber) and in all statistical combinations. The pH of the culture medium was fixed at 5.8 ± 0.1 and gelified with 5.5 g L-1 of agar before autoclaving at 121ºC and 1 atm for 20 min. After 150 days, an anatomical analysis was performed on cross-sections of the plant leaves. A complete randomized design was used. Modifications occurred on the plants of those treatments containing silicon compared to those without silicon, and between environment fo artificial and natural light. Plants showed larger growth at the artificial light treatment with 0.5 and 2.0 mg L-1 of calcium silicate respectively for the native and hybrid plants Treatments without silicate application induced chlorenchyma and epidermis deformation compared to silicon containing treatments, this effect can affect directly or indirectly plant growth at no silicon conditions. |
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Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062microscopyOrchidaceaein vitro culturecalcium silicatemicroscopyOrchidaceaein vitro culturecalcium silicateResearch on anatomical modifications under in vitro culture is essential to the definition and understanding of the development of micropropagated plants. Likewise, such research is essential to improve the steps of the acclimatization process. Accordingly, the objective of the present study is to verify the differences in the leaves anatomical traits of micropropagated orchids under calcium silicate concentrations. Seedlings of in vitro-germinated seeds, measuring 0.5 cm in length, were inoculated in 250 cm3 pots with 60 mL MS culture medium and different silicon concentrations (0, 0.5, 1.0 and 2.0 mg L-1) in different culture environments (natural environment, in a greenhouse and an artificial environment in a growth chamber) and in all statistical combinations. The pH of the culture medium was fixed at 5.8 ± 0.1 and gelified with 5.5 g L-1 of agar before autoclaving at 121ºC and 1 atm for 20 min. After 150 days, an anatomical analysis was performed on cross-sections of the plant leaves. A complete randomized design was used. Modifications occurred on the plants of those treatments containing silicon compared to those without silicon, and between environment fo artificial and natural light. Plants showed larger growth at the artificial light treatment with 0.5 and 2.0 mg L-1 of calcium silicate respectively for the native and hybrid plants Treatments without silicate application induced chlorenchyma and epidermis deformation compared to silicon containing treatments, this effect can affect directly or indirectly plant growth at no silicon conditions. Research on anatomical modifications under in vitro culture is essential to the definition and understanding of the development of micropropagated plants. Likewise, such research is essential to improve the steps of the acclimatization process. Accordingly, the objective of the present study is to verify the differences in the leaves anatomical traits of micropropagated orchids under calcium silicate concentrations. Seedlings of in vitro-germinated seeds, measuring 0.5 cm in length, were inoculated in 250 cm3 pots with 60 mL MS culture medium and different silicon concentrations (0, 0.5, 1.0 and 2.0 mg L-1) in different culture environments (natural environment, in a greenhouse and an artificial environment in a growth chamber) and in all statistical combinations. The pH of the culture medium was fixed at 5.8 ± 0.1 and gelified with 5.5 g L-1 of agar before autoclaving at 121ºC and 1 atm for 20 min. After 150 days, an anatomical analysis was performed on cross-sections of the plant leaves. A complete randomized design was used. Modifications occurred on the plants of those treatments containing silicon compared to those without silicon, and between environment fo artificial and natural light. Plants showed larger growth at the artificial light treatment with 0.5 and 2.0 mg L-1 of calcium silicate respectively for the native and hybrid plants Treatments without silicate application induced chlorenchyma and epidermis deformation compared to silicon containing treatments, this effect can affect directly or indirectly plant growth at no silicon conditions. Universidade Estadual de Maringá2012-04-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheetapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1506210.4025/actasciagron.v34i4.15062Acta Scientiarum. Agronomy; Vol 34 No 4 (2012); 413-421Acta Scientiarum. Agronomy; v. 34 n. 4 (2012); 413-4211807-86211679-9275reponame:Acta Scientiarum. Agronomy (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062/pdf_1http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062/751375141402http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062/751375141403Soares, Joyce Dória RodriguesPasqual, MoacirAraujo, Aparecida Gomes deCastro, Evaristo Mauro dePereira, Fabricio JoséBraga, Francyane Tavaresinfo:eu-repo/semantics/openAccess2022-11-23T18:38:33Zoai:periodicos.uem.br/ojs:article/15062Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgronPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/oaiactaagron@uem.br||actaagron@uem.br|| edamasio@uem.br1807-86211679-9275opendoar:2022-11-23T18:38:33Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 |
title |
Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 |
spellingShingle |
Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 Soares, Joyce Dória Rodrigues microscopy Orchidaceae in vitro culture calcium silicate microscopy Orchidaceae in vitro culture calcium silicate |
title_short |
Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 |
title_full |
Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 |
title_fullStr |
Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 |
title_full_unstemmed |
Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 |
title_sort |
Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062 |
author |
Soares, Joyce Dória Rodrigues |
author_facet |
Soares, Joyce Dória Rodrigues Pasqual, Moacir Araujo, Aparecida Gomes de Castro, Evaristo Mauro de Pereira, Fabricio José Braga, Francyane Tavares |
author_role |
author |
author2 |
Pasqual, Moacir Araujo, Aparecida Gomes de Castro, Evaristo Mauro de Pereira, Fabricio José Braga, Francyane Tavares |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Soares, Joyce Dória Rodrigues Pasqual, Moacir Araujo, Aparecida Gomes de Castro, Evaristo Mauro de Pereira, Fabricio José Braga, Francyane Tavares |
dc.subject.por.fl_str_mv |
microscopy Orchidaceae in vitro culture calcium silicate microscopy Orchidaceae in vitro culture calcium silicate |
topic |
microscopy Orchidaceae in vitro culture calcium silicate microscopy Orchidaceae in vitro culture calcium silicate |
description |
Research on anatomical modifications under in vitro culture is essential to the definition and understanding of the development of micropropagated plants. Likewise, such research is essential to improve the steps of the acclimatization process. Accordingly, the objective of the present study is to verify the differences in the leaves anatomical traits of micropropagated orchids under calcium silicate concentrations. Seedlings of in vitro-germinated seeds, measuring 0.5 cm in length, were inoculated in 250 cm3 pots with 60 mL MS culture medium and different silicon concentrations (0, 0.5, 1.0 and 2.0 mg L-1) in different culture environments (natural environment, in a greenhouse and an artificial environment in a growth chamber) and in all statistical combinations. The pH of the culture medium was fixed at 5.8 ± 0.1 and gelified with 5.5 g L-1 of agar before autoclaving at 121ºC and 1 atm for 20 min. After 150 days, an anatomical analysis was performed on cross-sections of the plant leaves. A complete randomized design was used. Modifications occurred on the plants of those treatments containing silicon compared to those without silicon, and between environment fo artificial and natural light. Plants showed larger growth at the artificial light treatment with 0.5 and 2.0 mg L-1 of calcium silicate respectively for the native and hybrid plants Treatments without silicate application induced chlorenchyma and epidermis deformation compared to silicon containing treatments, this effect can affect directly or indirectly plant growth at no silicon conditions. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062 10.4025/actasciagron.v34i4.15062 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062 |
identifier_str_mv |
10.4025/actasciagron.v34i4.15062 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062/pdf_1 http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062/751375141402 http://www.periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062/751375141403 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/vnd.openxmlformats-officedocument.spreadsheetml.sheet application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Agronomy; Vol 34 No 4 (2012); 413-421 Acta Scientiarum. Agronomy; v. 34 n. 4 (2012); 413-421 1807-8621 1679-9275 reponame:Acta Scientiarum. Agronomy (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum. Agronomy (Online) |
collection |
Acta Scientiarum. Agronomy (Online) |
repository.name.fl_str_mv |
Acta Scientiarum. Agronomy (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
actaagron@uem.br||actaagron@uem.br|| edamasio@uem.br |
_version_ |
1799305908301332480 |