Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.scienta.2020.109272 http://hdl.handle.net/11449/201547 |
Resumo: | Silicon has been added to the in vitro culture medium of orchids as a beneficial element that can successfully improve tissue culture. However, although supplemental Si may induce toxicity to the orchid culture depending on the source, there are no reports on the toxic concentrations of this element. To this end, this study conducted two experiments with two species of orchids, whose seedlings were obtained by sowing capsules containing mature seeds under aseptic conditions. At 120 days after sowing (DAS), seedlings of the Cymbidium atropurpureo and Dendrobium secundum orchids were recultivated in MS medium plus treatments in a 3 × 5 factorial scheme, with three silicon sources (nanosilica, potassium silicate and sodium silicate mixture, and monosilicic acid) at five different concentrations: 0.00 (control), 14.15, 28.30, 42.45, and 56.6 mmol L−1 Si following a completely randomized design with four repetitions and 10 seedlings each. The orchid culture ended after 300 DAS and the seedlings were removed from the flasks to evaluate the Si accumulation in plant tissues, the physiological data, pigments, biometric data and the survival percentage after acclimatization of 20 seedlings from each treatment. The results show that Si added to in vitro orchid cultivation may either favor growth or cause toxicity depending on the element source and concentration in the culture medium and the orchid species. Nanosilica at the 21.4 mmol L−1 Si concentration was the most promising source for orchid cultivation for Cymbidium atropurpureo. Further, monosilicic acid was considered the most toxic source due to the physiological damage caused to both Cymbidium atropurpureo and Dendrobium secundum at concentrations higher than 14.1 and 11.2 mmol L−1 Si, respectively. |
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Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchidsCymbidium atropurpureo and Dendrobium secundumMonosilicic acidNanosilicaOrchidaceaeSilicateSilicon has been added to the in vitro culture medium of orchids as a beneficial element that can successfully improve tissue culture. However, although supplemental Si may induce toxicity to the orchid culture depending on the source, there are no reports on the toxic concentrations of this element. To this end, this study conducted two experiments with two species of orchids, whose seedlings were obtained by sowing capsules containing mature seeds under aseptic conditions. At 120 days after sowing (DAS), seedlings of the Cymbidium atropurpureo and Dendrobium secundum orchids were recultivated in MS medium plus treatments in a 3 × 5 factorial scheme, with three silicon sources (nanosilica, potassium silicate and sodium silicate mixture, and monosilicic acid) at five different concentrations: 0.00 (control), 14.15, 28.30, 42.45, and 56.6 mmol L−1 Si following a completely randomized design with four repetitions and 10 seedlings each. The orchid culture ended after 300 DAS and the seedlings were removed from the flasks to evaluate the Si accumulation in plant tissues, the physiological data, pigments, biometric data and the survival percentage after acclimatization of 20 seedlings from each treatment. The results show that Si added to in vitro orchid cultivation may either favor growth or cause toxicity depending on the element source and concentration in the culture medium and the orchid species. Nanosilica at the 21.4 mmol L−1 Si concentration was the most promising source for orchid cultivation for Cymbidium atropurpureo. Further, monosilicic acid was considered the most toxic source due to the physiological damage caused to both Cymbidium atropurpureo and Dendrobium secundum at concentrations higher than 14.1 and 11.2 mmol L−1 Si, respectively.Universidade Estadual Paulista (Unesp) School of Agrarian and Veterinary Sciences, Via de Acesso Professor Paulo Donato Castellane, s/noUniversidade Estadual Paulista (Unesp) School of Agrarian and Veterinary Sciences, Via de Acesso Professor Paulo Donato Castellane, s/noUniversidade Estadual Paulista (Unesp)Mantovani, Cibele [UNESP]Pivetta, Kathia Fernandes Lopes [UNESP]de Mello Prado, Renato [UNESP]de Souza, Jonas Pereira [UNESP]Nascimento, Carolina Seno [UNESP]Nascimento, Camila Seno [UNESP]Gratão, Priscila Lupino [UNESP]2020-12-12T02:35:25Z2020-12-12T02:35:25Z2020-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.scienta.2020.109272Scientia Horticulturae, v. 265.0304-4238http://hdl.handle.net/11449/20154710.1016/j.scienta.2020.1092722-s2.0-85079223289Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientia Horticulturaeinfo:eu-repo/semantics/openAccess2024-06-07T13:56:42Zoai:repositorio.unesp.br:11449/201547Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T13:56:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids |
title |
Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids |
spellingShingle |
Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids Mantovani, Cibele [UNESP] Cymbidium atropurpureo and Dendrobium secundum Monosilicic acid Nanosilica Orchidaceae Silicate |
title_short |
Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids |
title_full |
Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids |
title_fullStr |
Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids |
title_full_unstemmed |
Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids |
title_sort |
Silicon toxicity induced by different concentrations and sources added to in vitro culture of epiphytic orchids |
author |
Mantovani, Cibele [UNESP] |
author_facet |
Mantovani, Cibele [UNESP] Pivetta, Kathia Fernandes Lopes [UNESP] de Mello Prado, Renato [UNESP] de Souza, Jonas Pereira [UNESP] Nascimento, Carolina Seno [UNESP] Nascimento, Camila Seno [UNESP] Gratão, Priscila Lupino [UNESP] |
author_role |
author |
author2 |
Pivetta, Kathia Fernandes Lopes [UNESP] de Mello Prado, Renato [UNESP] de Souza, Jonas Pereira [UNESP] Nascimento, Carolina Seno [UNESP] Nascimento, Camila Seno [UNESP] Gratão, Priscila Lupino [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mantovani, Cibele [UNESP] Pivetta, Kathia Fernandes Lopes [UNESP] de Mello Prado, Renato [UNESP] de Souza, Jonas Pereira [UNESP] Nascimento, Carolina Seno [UNESP] Nascimento, Camila Seno [UNESP] Gratão, Priscila Lupino [UNESP] |
dc.subject.por.fl_str_mv |
Cymbidium atropurpureo and Dendrobium secundum Monosilicic acid Nanosilica Orchidaceae Silicate |
topic |
Cymbidium atropurpureo and Dendrobium secundum Monosilicic acid Nanosilica Orchidaceae Silicate |
description |
Silicon has been added to the in vitro culture medium of orchids as a beneficial element that can successfully improve tissue culture. However, although supplemental Si may induce toxicity to the orchid culture depending on the source, there are no reports on the toxic concentrations of this element. To this end, this study conducted two experiments with two species of orchids, whose seedlings were obtained by sowing capsules containing mature seeds under aseptic conditions. At 120 days after sowing (DAS), seedlings of the Cymbidium atropurpureo and Dendrobium secundum orchids were recultivated in MS medium plus treatments in a 3 × 5 factorial scheme, with three silicon sources (nanosilica, potassium silicate and sodium silicate mixture, and monosilicic acid) at five different concentrations: 0.00 (control), 14.15, 28.30, 42.45, and 56.6 mmol L−1 Si following a completely randomized design with four repetitions and 10 seedlings each. The orchid culture ended after 300 DAS and the seedlings were removed from the flasks to evaluate the Si accumulation in plant tissues, the physiological data, pigments, biometric data and the survival percentage after acclimatization of 20 seedlings from each treatment. The results show that Si added to in vitro orchid cultivation may either favor growth or cause toxicity depending on the element source and concentration in the culture medium and the orchid species. Nanosilica at the 21.4 mmol L−1 Si concentration was the most promising source for orchid cultivation for Cymbidium atropurpureo. Further, monosilicic acid was considered the most toxic source due to the physiological damage caused to both Cymbidium atropurpureo and Dendrobium secundum at concentrations higher than 14.1 and 11.2 mmol L−1 Si, respectively. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:35:25Z 2020-12-12T02:35:25Z 2020-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.scienta.2020.109272 Scientia Horticulturae, v. 265. 0304-4238 http://hdl.handle.net/11449/201547 10.1016/j.scienta.2020.109272 2-s2.0-85079223289 |
url |
http://dx.doi.org/10.1016/j.scienta.2020.109272 http://hdl.handle.net/11449/201547 |
identifier_str_mv |
Scientia Horticulturae, v. 265. 0304-4238 10.1016/j.scienta.2020.109272 2-s2.0-85079223289 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientia Horticulturae |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045455823634432 |