Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7546 |
Resumo: | The enzymatic hydrolysis of oils and fats (or lipolysis) is a technological process that allows the attainment of fatty acids with high aggregate value and low energy consumption. Lipases are enzymes of vegetal, animal or microbial origin that catalyze total or partial hydrolysis of oils and fats. This work had as objective to verify the action of commercial lipase in the hydrolysis reaction of the sunflower oil and corn oil, at time of 24h. Analyses of the main parameters are evaluated (pH, time, temperature and concentration of the substrate), in order to express the maximum of its catalytic activities. The ideal time for better expression of activity in the two substrates was 5 min. It was found that the optimum pH for both substrates was 7.5. The optimum temperature was 50°C in sunflower oil and 40°C in corn oil. The effect of substrate concentration on the activity was at a concentration of 50% for sunflower oil and 30% for corn oil. The best production of total fatty acids was 36.52 g L-1 using sunflower oil and 29.05 g L-1 with the corn oil, over a period of 15h of reaction. |
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Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546Avaliação e comparação da eficiência de imobilização de lipase pancreática em quitosana para produção de ácidos graxos em frascos agitados - DOI: 10.4025/actascitechnol.v32i1.7546enzymesessential fatty acidshydrolysisenzimasácidos graxos essenciaishidróliseThe enzymatic hydrolysis of oils and fats (or lipolysis) is a technological process that allows the attainment of fatty acids with high aggregate value and low energy consumption. Lipases are enzymes of vegetal, animal or microbial origin that catalyze total or partial hydrolysis of oils and fats. This work had as objective to verify the action of commercial lipase in the hydrolysis reaction of the sunflower oil and corn oil, at time of 24h. Analyses of the main parameters are evaluated (pH, time, temperature and concentration of the substrate), in order to express the maximum of its catalytic activities. The ideal time for better expression of activity in the two substrates was 5 min. It was found that the optimum pH for both substrates was 7.5. The optimum temperature was 50°C in sunflower oil and 40°C in corn oil. The effect of substrate concentration on the activity was at a concentration of 50% for sunflower oil and 30% for corn oil. The best production of total fatty acids was 36.52 g L-1 using sunflower oil and 29.05 g L-1 with the corn oil, over a period of 15h of reaction.A hidrólise enzimática de óleos e gorduras ou lipólise é um processo tecnológico que permite a obtenção de ácidos graxos com alto valor agregado e baixo consumo energético. Lipases são enzimas de origem vegetal, animal ou microbiana, que catalisam a hidrólise total ou parcial de óleos e gorduras. Neste estudo, avaliou-se a ação da lipase comercial (pancreatina) na reação de hidrólise do óleo de girassol e de milho, num período de 24h. Foram avaliados os principais parâmetros (pH, tempo, temperatura e concentração do substrato), visando expressar, ao máximo, suas atividades catalíticas. O tempo ideal para melhor expressão enzimática nos dois substratos foi de 5 min. Verificou-se que o pH ótimo nos dois substratos utilizados foi de 7,5. A temperatura ótima foi de 50ºC no óleo de girassol e 40ºC no óleo de milho. O efeito da concentração do substrato sobre a atividade foi na concentração de 50% para o óleo de girassol e de 30% para o óleo de milho. A melhor produção de ácidos graxos totais foi de 36,52 g L-1, utilizando o óleo de girassol e de 29,05 g L-1, com o óleo de milho num período de 15h de reação.Universidade Estadual De Maringá2009-11-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/754610.4025/actascitechnol.v32i1.7546Acta Scientiarum. Technology; Vol 32 No 1 (2010); 15-19Acta Scientiarum. Technology; v. 32 n. 1 (2010); 15-191806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7546/7546Aguiar, Rafael Oliveira deMondardo, Ramiro MacariniAgnes, Eduardo JoãoCastro, Heizir Ferreira dePereira, Ernandes Beneditoinfo:eu-repo/semantics/openAccess2024-05-17T13:03:10Zoai:periodicos.uem.br/ojs:article/7546Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:03:10Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546 Avaliação e comparação da eficiência de imobilização de lipase pancreática em quitosana para produção de ácidos graxos em frascos agitados - DOI: 10.4025/actascitechnol.v32i1.7546 |
title |
Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546 |
spellingShingle |
Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546 Aguiar, Rafael Oliveira de enzymes essential fatty acids hydrolysis enzimas ácidos graxos essenciais hidrólise |
title_short |
Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546 |
title_full |
Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546 |
title_fullStr |
Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546 |
title_full_unstemmed |
Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546 |
title_sort |
Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking - DOI: 10.4025/actascitechnol.v32i1.7546 |
author |
Aguiar, Rafael Oliveira de |
author_facet |
Aguiar, Rafael Oliveira de Mondardo, Ramiro Macarini Agnes, Eduardo João Castro, Heizir Ferreira de Pereira, Ernandes Benedito |
author_role |
author |
author2 |
Mondardo, Ramiro Macarini Agnes, Eduardo João Castro, Heizir Ferreira de Pereira, Ernandes Benedito |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Aguiar, Rafael Oliveira de Mondardo, Ramiro Macarini Agnes, Eduardo João Castro, Heizir Ferreira de Pereira, Ernandes Benedito |
dc.subject.por.fl_str_mv |
enzymes essential fatty acids hydrolysis enzimas ácidos graxos essenciais hidrólise |
topic |
enzymes essential fatty acids hydrolysis enzimas ácidos graxos essenciais hidrólise |
description |
The enzymatic hydrolysis of oils and fats (or lipolysis) is a technological process that allows the attainment of fatty acids with high aggregate value and low energy consumption. Lipases are enzymes of vegetal, animal or microbial origin that catalyze total or partial hydrolysis of oils and fats. This work had as objective to verify the action of commercial lipase in the hydrolysis reaction of the sunflower oil and corn oil, at time of 24h. Analyses of the main parameters are evaluated (pH, time, temperature and concentration of the substrate), in order to express the maximum of its catalytic activities. The ideal time for better expression of activity in the two substrates was 5 min. It was found that the optimum pH for both substrates was 7.5. The optimum temperature was 50°C in sunflower oil and 40°C in corn oil. The effect of substrate concentration on the activity was at a concentration of 50% for sunflower oil and 30% for corn oil. The best production of total fatty acids was 36.52 g L-1 using sunflower oil and 29.05 g L-1 with the corn oil, over a period of 15h of reaction. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7546 10.4025/actascitechnol.v32i1.7546 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7546 |
identifier_str_mv |
10.4025/actascitechnol.v32i1.7546 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/7546/7546 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 32 No 1 (2010); 15-19 Acta Scientiarum. Technology; v. 32 n. 1 (2010); 15-19 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315333731844096 |