Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/63415 |
Resumo: | Studying new microbial sources and evaluating the growth kinetics behavior to predict large-scale production become essential. Besides, research with low-cost culture media has gained interest due to the need to decrease enzyme production costs. The literature concerning the use of macromycetes as a source of amylases, and applying mathematical models to predict their behavior is scarce. In this study, four growth kinetics in different compositions of culture media were carried out: two of them using different concentrations of Low-Grade Flour (LGF), one wheat mill by-product and two synthetic ones. In the kinetics mathematical models were used to evaluate the bioprocesses and their scaling feasibility. Mathematical models, such as modified Logistic, modified Gompertz, Baranyi, and Roberts, and modified Luedeking-Piret, were applied to predict mycelial growth, enzyme production, and substrate consumption. The results showed the model’s capability to predict mycelial growth (biomass) and substrate consumption (starch concentration). On the other hand, the mathematical models efficiently describe only the maximum enzymatic activity (α-amylase), not the process's other parameters, because of the complexity and diversity of biomolecules synthesized by Coprinus comatus. The wheat mill by-product was efficient as an ingredient in the culture media composition. Furthermore, it was concluded that B2 formulation (culture medium with 48.5 g/L of LGF) is the batch with the highest potential for the α-amylase production from C. comatus and the expansion of this process on an industrial scale. This study is important and valid for the scientific community since modeling studies for macromycetes are scarce in the literature. |
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Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium basidiomycetes; kinetics; biomass production; enzyme activity; substrate consumption.basidiomycetes; kinetics; biomass production; enzyme activity; substrate consumption.Studying new microbial sources and evaluating the growth kinetics behavior to predict large-scale production become essential. Besides, research with low-cost culture media has gained interest due to the need to decrease enzyme production costs. The literature concerning the use of macromycetes as a source of amylases, and applying mathematical models to predict their behavior is scarce. In this study, four growth kinetics in different compositions of culture media were carried out: two of them using different concentrations of Low-Grade Flour (LGF), one wheat mill by-product and two synthetic ones. In the kinetics mathematical models were used to evaluate the bioprocesses and their scaling feasibility. Mathematical models, such as modified Logistic, modified Gompertz, Baranyi, and Roberts, and modified Luedeking-Piret, were applied to predict mycelial growth, enzyme production, and substrate consumption. The results showed the model’s capability to predict mycelial growth (biomass) and substrate consumption (starch concentration). On the other hand, the mathematical models efficiently describe only the maximum enzymatic activity (α-amylase), not the process's other parameters, because of the complexity and diversity of biomolecules synthesized by Coprinus comatus. The wheat mill by-product was efficient as an ingredient in the culture media composition. Furthermore, it was concluded that B2 formulation (culture medium with 48.5 g/L of LGF) is the batch with the highest potential for the α-amylase production from C. comatus and the expansion of this process on an industrial scale. This study is important and valid for the scientific community since modeling studies for macromycetes are scarce in the literature.Studying new microbial sources and evaluating the growth kinetics behavior to predict large-scale production become essential. Besides, research with low-cost culture media has gained interest due to the need to decrease enzyme production costs. The literature concerning the use of macromycetes as a source of amylases, and applying mathematical models to predict their behavior is scarce. In this study, four growth kinetics in different compositions of culture media were carried out: two of them using different concentrations of Low-Grade Flour (LGF), one wheat mill by-product and two synthetic ones. In the kinetics mathematical models were used to evaluate the bioprocesses and their scaling feasibility. Mathematical models, such as modified Logistic, modified Gompertz, Baranyi, and Roberts, and modified Luedeking-Piret, were applied to predict mycelial growth, enzyme production, and substrate consumption. The results showed the model’s capability to predict mycelial growth (biomass) and substrate consumption (starch concentration). On the other hand, the mathematical models efficiently describe only the maximum enzymatic activity (α-amylase), not the process's other parameters, because of the complexity and diversity of biomolecules synthesized by Coprinus comatus. The wheat mill by-product was efficient as an ingredient in the culture media composition. Furthermore, it was concluded that B2 formulation (culture medium with 48.5 g/L of LGF) is the batch with the highest potential for the α-amylase production from C. comatus and the expansion of this process on an industrial scale. This study is important and valid for the scientific community since modeling studies for macromycetes are scarce in the literature.Universidade Estadual De Maringá2023-05-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/6341510.4025/actascitechnol.v45i1.63415Acta Scientiarum. Technology; Vol 45 (2023): Publicação contínua; e63415Acta Scientiarum. Technology; v. 45 (2023): Publicação contínua; e634151806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/63415/751375155856Copyright (c) 2023 Acta Scientiarum. Technologyhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPaludo, Luana CristinaBiz, Ana Paula Gonçalves, Kamilla MarquesStrobel, Christian ScapulatempoVaz, Michelle Rossana FerreiraSpier, Michele Rigon2023-05-25T13:56:36Zoai:periodicos.uem.br/ojs:article/63415Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2023-05-25T13:56:36Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium |
title |
Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium |
spellingShingle |
Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium Paludo, Luana Cristina basidiomycetes; kinetics; biomass production; enzyme activity; substrate consumption. basidiomycetes; kinetics; biomass production; enzyme activity; substrate consumption. |
title_short |
Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium |
title_full |
Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium |
title_fullStr |
Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium |
title_full_unstemmed |
Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium |
title_sort |
Validation of mathematical models as a tool for prediction of α-amylase production by Coprinus comatus in a low-cost culture medium |
author |
Paludo, Luana Cristina |
author_facet |
Paludo, Luana Cristina Biz, Ana Paula Gonçalves, Kamilla Marques Strobel, Christian Scapulatempo Vaz, Michelle Rossana Ferreira Spier, Michele Rigon |
author_role |
author |
author2 |
Biz, Ana Paula Gonçalves, Kamilla Marques Strobel, Christian Scapulatempo Vaz, Michelle Rossana Ferreira Spier, Michele Rigon |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Paludo, Luana Cristina Biz, Ana Paula Gonçalves, Kamilla Marques Strobel, Christian Scapulatempo Vaz, Michelle Rossana Ferreira Spier, Michele Rigon |
dc.subject.por.fl_str_mv |
basidiomycetes; kinetics; biomass production; enzyme activity; substrate consumption. basidiomycetes; kinetics; biomass production; enzyme activity; substrate consumption. |
topic |
basidiomycetes; kinetics; biomass production; enzyme activity; substrate consumption. basidiomycetes; kinetics; biomass production; enzyme activity; substrate consumption. |
description |
Studying new microbial sources and evaluating the growth kinetics behavior to predict large-scale production become essential. Besides, research with low-cost culture media has gained interest due to the need to decrease enzyme production costs. The literature concerning the use of macromycetes as a source of amylases, and applying mathematical models to predict their behavior is scarce. In this study, four growth kinetics in different compositions of culture media were carried out: two of them using different concentrations of Low-Grade Flour (LGF), one wheat mill by-product and two synthetic ones. In the kinetics mathematical models were used to evaluate the bioprocesses and their scaling feasibility. Mathematical models, such as modified Logistic, modified Gompertz, Baranyi, and Roberts, and modified Luedeking-Piret, were applied to predict mycelial growth, enzyme production, and substrate consumption. The results showed the model’s capability to predict mycelial growth (biomass) and substrate consumption (starch concentration). On the other hand, the mathematical models efficiently describe only the maximum enzymatic activity (α-amylase), not the process's other parameters, because of the complexity and diversity of biomolecules synthesized by Coprinus comatus. The wheat mill by-product was efficient as an ingredient in the culture media composition. Furthermore, it was concluded that B2 formulation (culture medium with 48.5 g/L of LGF) is the batch with the highest potential for the α-amylase production from C. comatus and the expansion of this process on an industrial scale. This study is important and valid for the scientific community since modeling studies for macromycetes are scarce in the literature. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/63415 10.4025/actascitechnol.v45i1.63415 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/63415 |
identifier_str_mv |
10.4025/actascitechnol.v45i1.63415 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/63415/751375155856 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Acta Scientiarum. Technology http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Acta Scientiarum. Technology http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 45 (2023): Publicação contínua; e63415 Acta Scientiarum. Technology; v. 45 (2023): Publicação contínua; e63415 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315338172563456 |