Extraction of total polyphenols from hibiscus (Hibiscus sabdariffa L.) and waxweed / ‘sete-sangrias’ (Cuphea carthagenensis) and evaluation of their antioxidant potential

• Autor(a) principal: Begmeier, Daniele
• Data de Publicação: 2014
• Outros Autores: Berres, Pedro Henrique Dalberto, Filippi, Débora, Bilibio, Denise, Bettiol, Vanderlei Rodrigo, Priamo, Wagner Luiz
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta scientiarum. Technology (Online)
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/19093
• topic: polyphenolic fraction; DPPH; natural extracts; Tecnologia de Alimentos

Current research investigates the extraction process of total polyphenols from hibiscus (Hibiscus sabdariffa L.) and waxweed (Brazilian name: ‘sete-sangrias’) (Cuphea carthagenensis) and evaluates the antioxidant potential of their extracts. The extraction stage comprised investigation on the following parameters: i) solvents (acetone and ethanol) pure and fractioned with water; ii) variables (temperature, stirring, solvent ratio, time and pH). Total polyphenols were quantified by Folin-Ciocalteau reagent and antioxidant activity was determined by ABTS•+ (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Results showed that, depending on experimental conditions, total phenolic contents for hibiscus and waxweed ranged between 460.86 mg GAE 100 g-1 and 5012.54 mg GAE 100 g-1 and between 462.86 mg GAE 100 g-1 and 4215.99 mg GAE 100 g-1, respectively. Waxweed had a higher antioxidant activity when compared to that of hibiscus by both ABTS•+ and DPPH. Data showed that hibiscus and waxweed have a significant amount of polyphenols which may be extracted in mild processing conditions and then employed as natural antioxidant sources in industrial processes.