Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta scientiarum. Technology (Online) |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/9400 |
Resumo: | Glycosaminoglycans (GAGs) comprise the extracellular matrix of animal tissues, and residues from processing fish commercially harvested may offer new anticoagulant agents to substitute the heparin (HEP) in medical sciences. The aim of this study was to purify, characterize physico-chemically and evaluate the anticoagulant activity of GAGs isolated from the skin of Nile tilapia, Oreochromis niloticus. The GAGs were extracted with papain in 100 mM sodium acetate buffer (pH 5.0) containing cystein and EDTA (5 mM), followed by ion-exchange chromatography on a DEAE-cellulose column using a NaCl gradient. The obtained fractions were lyophilizated and submitted to 0.5% agarose gel electrophoresis. The anticoagulant activity was assessed by the activated partial thromboplastin time (APTT) using normal human plasma and HEP standard (193 IU mg-1). The chromatographic profile separated into two different GAGs fractions (F I and F II) eluted at 0.50 and 0.75 M of NaCl, respectively, revealing fractions pattern distinct in the charge densities, but both showing GAGs with mobilities similar to standard dermatan sulfate (DS), suggesting the preliminary identification of this GAG. The GAGs modified the APTT, whose activities were, respectively, 4.72 (F I) and 23.80 (F II) IU mg-1, and expressed themselves dose-dependent. Therefore, the anticoagulant DS from O. niloticus suggests an interesting source for posterior studies of antithrombotic activity. |
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Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400Purificação, caracterização físico-química e atividade anticoagulante de glicosaminoglicanos isolados da pele de tilápia do Nilo (Oreochromis niloticus) - doi: 10.4025/actascitechnol.v33i3.9400sulfated heteropolysaccharidesOreochromis niloticusblood coagulationAPTT testheteropolissacarídeos sulfatadosOreochromis niloticuscoagulação sanguíneateste do TTPA2.08.01.00-9Glycosaminoglycans (GAGs) comprise the extracellular matrix of animal tissues, and residues from processing fish commercially harvested may offer new anticoagulant agents to substitute the heparin (HEP) in medical sciences. The aim of this study was to purify, characterize physico-chemically and evaluate the anticoagulant activity of GAGs isolated from the skin of Nile tilapia, Oreochromis niloticus. The GAGs were extracted with papain in 100 mM sodium acetate buffer (pH 5.0) containing cystein and EDTA (5 mM), followed by ion-exchange chromatography on a DEAE-cellulose column using a NaCl gradient. The obtained fractions were lyophilizated and submitted to 0.5% agarose gel electrophoresis. The anticoagulant activity was assessed by the activated partial thromboplastin time (APTT) using normal human plasma and HEP standard (193 IU mg-1). The chromatographic profile separated into two different GAGs fractions (F I and F II) eluted at 0.50 and 0.75 M of NaCl, respectively, revealing fractions pattern distinct in the charge densities, but both showing GAGs with mobilities similar to standard dermatan sulfate (DS), suggesting the preliminary identification of this GAG. The GAGs modified the APTT, whose activities were, respectively, 4.72 (F I) and 23.80 (F II) IU mg-1, and expressed themselves dose-dependent. Therefore, the anticoagulant DS from O. niloticus suggests an interesting source for posterior studies of antithrombotic activity.Os glicosaminoglicanos (GAGs) constituem a matriz extracelular de tecidos animais e os resíduos do processamento de peixes comercialmente cultivados podem oferecer novos agentes anticoagulantes em substituição à heparina (HEP) nas ciências médicas. Objetivou-se purificar, caracterizar físico-quimicamente e avaliar a atividade anticoagulante dos GAGs isolados da pele da tilápia do Nilo, Oreochromis niloticus. Os GAGs foram extraídos com papaína em tampão acetato de sódio 100 mM (pH 5,0) contendo cisteína e EDTA (5 mM), seguido por cromatografia de troca iônica em coluna de DEAE-celulose, utilizando um gradiente de NaCl. As frações obtidas foram liofilizadas e submetidas à eletroforese em gel de agarose a 0,5%. A atividade anticoagulante foi avaliada por meio do tempo de tromboplastina parcial ativada (TTPA), usando-se plasma humano normal e HEP padrão (193 UI mg-1). O perfil cromatográfico mostrou a separação de duas diferentes frações de GAGs (F I e F II), eluídas com 0,50 e 0,75 M de NaCl, respectivamente, revelando, por eletroforese, frações distintas em densidade de cargas, porém ambas mostrando GAGs com mobilidades semelhantes ao dermatam sulfato (DS) padrão, sugerindo-se a identificação preliminar desse GAG. Os GAGs modificaram o TTPA, cujas atividades perfizeram, respectivamente, 4,72 (F I) e 23,80 (F II) UI mg-1, e expressaram-se dose-dependente. Portanto, o DS anticoagulante da pele de O. niloticus sugere uma fonte interessante para estudos posteriores de atividade antitrombótica.Universidade Estadual De Maringá2011-03-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBioprospecçãoapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/940010.4025/actascitechnol.v33i3.9400Acta Scientiarum. Technology; Vol 33 No 3 (2011); 233-241Acta Scientiarum. Technology; v. 33 n. 3 (2011); 233-2411806-25631807-8664reponame:Acta scientiarum. Technology (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/9400/9400Rodrigues, José Ariévilo GurgelQuinderé, Ana Luíza GomesQueiroz, Ismael Nilo Lino deCoura, Chistiane OliveiraAraújo, Glácio SouzaBenevides, Norma Maria Barrosinfo:eu-repo/semantics/openAccess2024-05-17T13:03:14Zoai:periodicos.uem.br/ojs:article/9400Revistahttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/indexPUBhttps://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/oai||actatech@uem.br1807-86641806-2563opendoar:2024-05-17T13:03:14Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400 Purificação, caracterização físico-química e atividade anticoagulante de glicosaminoglicanos isolados da pele de tilápia do Nilo (Oreochromis niloticus) - doi: 10.4025/actascitechnol.v33i3.9400 |
title |
Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400 |
spellingShingle |
Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400 Rodrigues, José Ariévilo Gurgel sulfated heteropolysaccharides Oreochromis niloticus blood coagulation APTT test heteropolissacarídeos sulfatados Oreochromis niloticus coagulação sanguínea teste do TTPA 2.08.01.00-9 |
title_short |
Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400 |
title_full |
Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400 |
title_fullStr |
Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400 |
title_full_unstemmed |
Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400 |
title_sort |
Purification, physical and chemical characterization, and anticoagulant activity of glycosaminoglycans isolated from the skin of Nile tilapia (Oreochromis niloticus). - doi: 10.4025/actascitechnol.v33i3.9400 |
author |
Rodrigues, José Ariévilo Gurgel |
author_facet |
Rodrigues, José Ariévilo Gurgel Quinderé, Ana Luíza Gomes Queiroz, Ismael Nilo Lino de Coura, Chistiane Oliveira Araújo, Glácio Souza Benevides, Norma Maria Barros |
author_role |
author |
author2 |
Quinderé, Ana Luíza Gomes Queiroz, Ismael Nilo Lino de Coura, Chistiane Oliveira Araújo, Glácio Souza Benevides, Norma Maria Barros |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, José Ariévilo Gurgel Quinderé, Ana Luíza Gomes Queiroz, Ismael Nilo Lino de Coura, Chistiane Oliveira Araújo, Glácio Souza Benevides, Norma Maria Barros |
dc.subject.por.fl_str_mv |
sulfated heteropolysaccharides Oreochromis niloticus blood coagulation APTT test heteropolissacarídeos sulfatados Oreochromis niloticus coagulação sanguínea teste do TTPA 2.08.01.00-9 |
topic |
sulfated heteropolysaccharides Oreochromis niloticus blood coagulation APTT test heteropolissacarídeos sulfatados Oreochromis niloticus coagulação sanguínea teste do TTPA 2.08.01.00-9 |
description |
Glycosaminoglycans (GAGs) comprise the extracellular matrix of animal tissues, and residues from processing fish commercially harvested may offer new anticoagulant agents to substitute the heparin (HEP) in medical sciences. The aim of this study was to purify, characterize physico-chemically and evaluate the anticoagulant activity of GAGs isolated from the skin of Nile tilapia, Oreochromis niloticus. The GAGs were extracted with papain in 100 mM sodium acetate buffer (pH 5.0) containing cystein and EDTA (5 mM), followed by ion-exchange chromatography on a DEAE-cellulose column using a NaCl gradient. The obtained fractions were lyophilizated and submitted to 0.5% agarose gel electrophoresis. The anticoagulant activity was assessed by the activated partial thromboplastin time (APTT) using normal human plasma and HEP standard (193 IU mg-1). The chromatographic profile separated into two different GAGs fractions (F I and F II) eluted at 0.50 and 0.75 M of NaCl, respectively, revealing fractions pattern distinct in the charge densities, but both showing GAGs with mobilities similar to standard dermatan sulfate (DS), suggesting the preliminary identification of this GAG. The GAGs modified the APTT, whose activities were, respectively, 4.72 (F I) and 23.80 (F II) IU mg-1, and expressed themselves dose-dependent. Therefore, the anticoagulant DS from O. niloticus suggests an interesting source for posterior studies of antithrombotic activity. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bioprospecção |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/9400 10.4025/actascitechnol.v33i3.9400 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/9400 |
identifier_str_mv |
10.4025/actascitechnol.v33i3.9400 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciTechnol/article/view/9400/9400 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Technology; Vol 33 No 3 (2011); 233-241 Acta Scientiarum. Technology; v. 33 n. 3 (2011); 233-241 1806-2563 1807-8664 reponame:Acta scientiarum. Technology (Online) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta scientiarum. Technology (Online) |
collection |
Acta scientiarum. Technology (Online) |
repository.name.fl_str_mv |
Acta scientiarum. Technology (Online) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actatech@uem.br |
_version_ |
1799315333833555968 |