Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056

Detalhes bibliográficos
Autor(a) principal: Rodrigues, José Ariévilo Gurgel
Data de Publicação: 2011
Outros Autores: Vanderlei, Edfranck de Sousa Oliveira, Quinderé, Ana Luíza Gomes, Fontes, Bruno Pedrosa, Queiroz, Ismael Nilo Lino de, Benevides, Norma Maria Barros
Tipo de documento: Artigo
Idioma: por
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056
Resumo: A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1.
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spelling Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056Glicosaminoglicanos isolados da pele de palombeta (Chloroscombrus chrysurus) e guaiúba (Ocyurus chrysurus): características e implicações biológicas - doi: 10.4025/actascibiolsci.v34i2.6056marine teleostssulfated glycosaminoglycansblood coagulationAPTT testteleósteos marinhosglicosaminoglicanos sulfatadoscoagulação sanguíneateste do TTPAQuímica de macromoléculasA great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1.O Brasil abriga uma das maiores biodiversidades marinhas do mundo, favorecendo a descoberta de fontes alternativas de compostos farmacológicos. Desta forma, objetivou-se avaliar o potencial anticoagulante de glicosaminoglicanos (GAGs) isolados das peles da palombeta (Chloroscombrus chrysurus) e guaiúba (Ocyurus chrysurus). Os GAGs foram extraídos com papaína bruta em tampão acetato de sódio 0,1 M (pH 5,0) contendo cisteína 5 mM e EDTA 5 mM, seguido por cromatografia de troca iônica do extrato total em coluna de DEAE-celulose. As frações obtidas foram analisadas quanto à composição química (proteínas contaminantes e carboidratos totais) e os GAGs identificados por eletroforese em gel de agarose a 0,5%. Os ensaios de atividade anticoagulante foram realizados por meio do tempo de tromboplastina parcial ativada (TTPA) usando plasma humano normal e heparina-padrão (193,00 UI mg-1). O procedimento de obtenção e fracionamento dos GAGs mostrou-se eficiente, indicando semelhantes perfis cromatográficos entre as espécies avaliadas e, revelando para C. chrysurus, bandas com mobilidades semelhantes ao dermatam sulfato e com atividade de apenas 3,30 UI mg-1.Universidade Estadual De Maringá2011-10-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBioprospecção ; composição bioquímica ; relações biológicasapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/605610.4025/actascibiolsci.v34i2.6056Acta Scientiarum. Biological Sciences; Vol 34 No 2 (2012); 141-148Acta Scientiarum. Biological Sciences; v. 34 n. 2 (2012); 141-1481807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056/pdfRodrigues, José Ariévilo GurgelVanderlei, Edfranck de Sousa OliveiraQuinderé, Ana Luíza GomesFontes, Bruno PedrosaQueiroz, Ismael Nilo Lino deBenevides, Norma Maria Barrosinfo:eu-repo/semantics/openAccess2022-11-23T17:33:51Zoai:periodicos.uem.br/ojs:article/6056Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:51Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
Glicosaminoglicanos isolados da pele de palombeta (Chloroscombrus chrysurus) e guaiúba (Ocyurus chrysurus): características e implicações biológicas - doi: 10.4025/actascibiolsci.v34i2.6056
title Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
spellingShingle Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
Rodrigues, José Ariévilo Gurgel
marine teleosts
sulfated glycosaminoglycans
blood coagulation
APTT test
teleósteos marinhos
glicosaminoglicanos sulfatados
coagulação sanguínea
teste do TTPA
Química de macromoléculas
title_short Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
title_full Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
title_fullStr Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
title_full_unstemmed Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
title_sort Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
author Rodrigues, José Ariévilo Gurgel
author_facet Rodrigues, José Ariévilo Gurgel
Vanderlei, Edfranck de Sousa Oliveira
Quinderé, Ana Luíza Gomes
Fontes, Bruno Pedrosa
Queiroz, Ismael Nilo Lino de
Benevides, Norma Maria Barros
author_role author
author2 Vanderlei, Edfranck de Sousa Oliveira
Quinderé, Ana Luíza Gomes
Fontes, Bruno Pedrosa
Queiroz, Ismael Nilo Lino de
Benevides, Norma Maria Barros
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues, José Ariévilo Gurgel
Vanderlei, Edfranck de Sousa Oliveira
Quinderé, Ana Luíza Gomes
Fontes, Bruno Pedrosa
Queiroz, Ismael Nilo Lino de
Benevides, Norma Maria Barros
dc.subject.por.fl_str_mv marine teleosts
sulfated glycosaminoglycans
blood coagulation
APTT test
teleósteos marinhos
glicosaminoglicanos sulfatados
coagulação sanguínea
teste do TTPA
Química de macromoléculas
topic marine teleosts
sulfated glycosaminoglycans
blood coagulation
APTT test
teleósteos marinhos
glicosaminoglicanos sulfatados
coagulação sanguínea
teste do TTPA
Química de macromoléculas
description A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-21
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Bioprospecção ; composição bioquímica ; relações biológicas
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056
10.4025/actascibiolsci.v34i2.6056
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056
identifier_str_mv 10.4025/actascibiolsci.v34i2.6056
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056/pdf
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 34 No 2 (2012); 141-148
Acta Scientiarum. Biological Sciences; v. 34 n. 2 (2012); 141-148
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
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