Induced reproduction of dourado (Salminus brasiliensis): fertilization with sperm cryopreserved in ACP®-104

• Autor(a) principal: Zanandrea, Ana Carolina Volpato
• Data de Publicação: 2016
• Outros Autores: Weingartner, Marcos, Zaniboni-Filho, Evoy
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Scientiarum. Animal Sciences (Online)
• Texto Completo: https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/28838
• topic: cryopreservation; sperm; fertilization; cryoprotectant; coconut water

Experiments were conducted to improve the fertilization rate of cryopreserved semen of dourado, Salminus brasiliensis. The experiment tested two cryoprotectant solutions at different semen: cryoprotectant ratios (1:5, 1:15, 1:25 and 1:50). The standard solution for the species (mixture of dimethyl sulfoxide, glucose, egg yolk and distilled water) was compared to a 350 mOsm ACP®-104 solution, which is composed of powdered coconut water diluted in distilled water and methylglycol. Differences between the dilutions tested were significant only for ACP®. The fertilization potential by using the standard solution at the lowest dilution (1:5) is equated when the sperm is diluted in ACP® at 1:25 or 1:50. These results show that the standard solution is the most suitable for the cryopreservation of dourado sperm, since the dilution did not alter the fertilization rate, requiring smaller storage space.