Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/1455 |
Resumo: | The sensitivity of dourado (Salminus brasiliensis) spermatozoa to different cryoprotectant solutions was evaluated in three experiments. In experiment 1, semen was diluted, 1:10, in 12 solutions (four extenders x three cryoprotectants - dimethylsulphoxide (DMSO), methyglycol, or glycerol). Half of each sample was refrigerated for one hour while the other half was cryopreserved. Sperm motility was immediately assessed after dilution and after refrigeration in all samples, and after thawing in those cryopreserved in DMSO. In experiment 2, semen was diluted, 1:5, in five solutions containing DMSO, refrigerated, cryopreserved, and analyzed as in experiment 1. In experiment 3, semen was diluted, 1:5, in five solutions containing DMSO, cryopreserved and evaluated for motility and fertility. When semen was diluted 1:10, motility higher than 58% was observed in all samples refrigerated in DMSO and in NaCl-tris-methylglycol. Low motility was observed in samples refrigerated in the other combinations of methylglycol (5-32%) or glycerol (0-8%) and in those cryopreserved (16-20%). All samples diluted 1:5 yielded motility of 65-72% after refrigeration, and 45-66% after thawing (experiments 2 and 3). The hatching rates produced with cryopreserved semen, however, were lower (17-23%) compared to fresh semen (60%). |
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Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetorasSensitivity of dourado (Salminus brasiliensis) spermatozoa to different cryoprotectant solutionsDouradoSalminus brasiliensisDiluidorCrioprotetorCriopreservaçãoFertilizaçãoExtenderCryoprotectantCryopreservationFertilizationThe sensitivity of dourado (Salminus brasiliensis) spermatozoa to different cryoprotectant solutions was evaluated in three experiments. In experiment 1, semen was diluted, 1:10, in 12 solutions (four extenders x three cryoprotectants - dimethylsulphoxide (DMSO), methyglycol, or glycerol). Half of each sample was refrigerated for one hour while the other half was cryopreserved. Sperm motility was immediately assessed after dilution and after refrigeration in all samples, and after thawing in those cryopreserved in DMSO. In experiment 2, semen was diluted, 1:5, in five solutions containing DMSO, refrigerated, cryopreserved, and analyzed as in experiment 1. In experiment 3, semen was diluted, 1:5, in five solutions containing DMSO, cryopreserved and evaluated for motility and fertility. When semen was diluted 1:10, motility higher than 58% was observed in all samples refrigerated in DMSO and in NaCl-tris-methylglycol. Low motility was observed in samples refrigerated in the other combinations of methylglycol (5-32%) or glycerol (0-8%) and in those cryopreserved (16-20%). All samples diluted 1:5 yielded motility of 65-72% after refrigeration, and 45-66% after thawing (experiments 2 and 3). The hatching rates produced with cryopreserved semen, however, were lower (17-23%) compared to fresh semen (60%).Em três experimentos, avaliou-se a sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras. No experimento 1, o sêmen foi diluído, 1:10, em 12 soluções (quatro diluidores x três crioprotetores – dimetilsulfóxido (DMSO), metilglicol ou glicerol). Metade de cada amostra foi resfriada por uma hora e a outra, criopreservada. A motilidade espermática foi avaliada imediatamente após a diluição e após o resfriamento em todas as amostras e, após o descongelamento, apenas nas amostras criopreservadas em DMSO. No experimento 2, o sêmen foi diluído, 1:5, em cinco soluções contendo DMSO e resfriado, criopreservado e avaliado como no experimento 1. No experimento 3, o sêmen foi diluído, 1:5, em quatro soluções contendo DMSO e criopreservado e avaliado quanto à motilidade e à fertilidade. Quando o sêmen foi diluído 1:10, observou-se motilidade acima de 58% em todas as amostras resfriadas em DMSO e em NaCl-tris-metilglicol. Baixa motilidade foi observada nas amostras resfriadas nas outras combinações com metilglicol (5-32%) ou glicerol (0-8%) e naquelas criopreservadas (16-20%). Todas as amostras diluídas 1:5 apresentaram motilidade de 65-72% após o resfriamento e de 45-66% após o descongelamento (experimentos 2 e 3). As taxas de eclosão produzidas com sêmen criopreservado, entretanto, foram baixas (17-23%) em relação ao sêmen fresco (60%).Universidade Federal de Minas Gerais2013-11-28T19:03:59Z2013-11-28T19:03:59Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVIVEIROS, A.T.M. et al. Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v.61, n.4, p.883-889, 2009.http://repositorio.ufla.br/jspui/handle/1/1455Arquivo Brasileiro de Medicina Veterinária e Zootecniareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAViveiros, Ana Tereza de MendonçaOliveira, A.V.Maria, A.N.Orfão, L.H.Souza, J.C.info:eu-repo/semantics/openAccesspor2016-12-15T17:43:05Zoai:localhost:1/1455Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-12-15T17:43:05Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras Sensitivity of dourado (Salminus brasiliensis) spermatozoa to different cryoprotectant solutions |
title |
Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras |
spellingShingle |
Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras Viveiros, Ana Tereza de Mendonça Dourado Salminus brasiliensis Diluidor Crioprotetor Criopreservação Fertilização Extender Cryoprotectant Cryopreservation Fertilization |
title_short |
Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras |
title_full |
Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras |
title_fullStr |
Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras |
title_full_unstemmed |
Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras |
title_sort |
Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras |
author |
Viveiros, Ana Tereza de Mendonça |
author_facet |
Viveiros, Ana Tereza de Mendonça Oliveira, A.V. Maria, A.N. Orfão, L.H. Souza, J.C. |
author_role |
author |
author2 |
Oliveira, A.V. Maria, A.N. Orfão, L.H. Souza, J.C. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Viveiros, Ana Tereza de Mendonça Oliveira, A.V. Maria, A.N. Orfão, L.H. Souza, J.C. |
dc.subject.por.fl_str_mv |
Dourado Salminus brasiliensis Diluidor Crioprotetor Criopreservação Fertilização Extender Cryoprotectant Cryopreservation Fertilization |
topic |
Dourado Salminus brasiliensis Diluidor Crioprotetor Criopreservação Fertilização Extender Cryoprotectant Cryopreservation Fertilization |
description |
The sensitivity of dourado (Salminus brasiliensis) spermatozoa to different cryoprotectant solutions was evaluated in three experiments. In experiment 1, semen was diluted, 1:10, in 12 solutions (four extenders x three cryoprotectants - dimethylsulphoxide (DMSO), methyglycol, or glycerol). Half of each sample was refrigerated for one hour while the other half was cryopreserved. Sperm motility was immediately assessed after dilution and after refrigeration in all samples, and after thawing in those cryopreserved in DMSO. In experiment 2, semen was diluted, 1:5, in five solutions containing DMSO, refrigerated, cryopreserved, and analyzed as in experiment 1. In experiment 3, semen was diluted, 1:5, in five solutions containing DMSO, cryopreserved and evaluated for motility and fertility. When semen was diluted 1:10, motility higher than 58% was observed in all samples refrigerated in DMSO and in NaCl-tris-methylglycol. Low motility was observed in samples refrigerated in the other combinations of methylglycol (5-32%) or glycerol (0-8%) and in those cryopreserved (16-20%). All samples diluted 1:5 yielded motility of 65-72% after refrigeration, and 45-66% after thawing (experiments 2 and 3). The hatching rates produced with cryopreserved semen, however, were lower (17-23%) compared to fresh semen (60%). |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2013-11-28T19:03:59Z 2013-11-28T19:03:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VIVEIROS, A.T.M. et al. Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v.61, n.4, p.883-889, 2009. http://repositorio.ufla.br/jspui/handle/1/1455 |
identifier_str_mv |
VIVEIROS, A.T.M. et al. Sensibilidade dos espermatozoides de dourado (Salminus brasiliensis) a diferentes soluções crioprotetoras. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v.61, n.4, p.883-889, 2009. |
url |
http://repositorio.ufla.br/jspui/handle/1/1455 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1784550145782710272 |