Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS

Detalhes bibliográficos
Autor(a) principal: Magnotti, Caio Cesar França
Data de Publicação: 2018
Outros Autores: Castro, Jorgélia de Jesus Pinto, Pedrotti, Fabiola Santiago, Sterzelecki, Fábio Carneiro, Sanches, Eduardo Gomes, Cerqueira, Vinicius Ronzani
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum. Animal Sciences (Online)
DOI: 10.4025/actascianimsci.v40i1.39563
Texto Completo: https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/39563
Resumo:  The present study aimed to evaluate the activation characteristics of the lebranche mullet spermatozoa in natura and diluted with CF-HBSS for 96h at 4±2°C. The semen was collected from eight wild fish in Florianópolis – SC (Brazil) (27°S) in May, during reproductive migration. Three pools of semen were divided into two treatments: in natura and diluted with CF-HBSS 1:3. The semen was activated with seawater (salinity of 34, pH 8.7 and 4±2°C) to determine: motility time, motility rate and sperm cell membrane integrity. Dilution with CF-HBSS 1:3 increased motility time of diluted semen (15-20% for 6, 12 and 18h). Motility rate was equal to fresh semen for up to 24h of refrigeration and it was 30% higher than in natura semen at 12, 18 and 24h. Cell membrane integrity was maintained in fresh semen up to 6h, and it was 15-20% higher than in natura semen from 6 to 48h (p < 0.05). The use of CF-HBSS as diluent in the ratio 1:3 is recommended for semen refrigeration of lebranche mullet. 
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spelling Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSSgamete conservationspermatozoaaquaculturemarine fish farmingnear threatened fish. The present study aimed to evaluate the activation characteristics of the lebranche mullet spermatozoa in natura and diluted with CF-HBSS for 96h at 4±2°C. The semen was collected from eight wild fish in Florianópolis – SC (Brazil) (27°S) in May, during reproductive migration. Three pools of semen were divided into two treatments: in natura and diluted with CF-HBSS 1:3. The semen was activated with seawater (salinity of 34, pH 8.7 and 4±2°C) to determine: motility time, motility rate and sperm cell membrane integrity. Dilution with CF-HBSS 1:3 increased motility time of diluted semen (15-20% for 6, 12 and 18h). Motility rate was equal to fresh semen for up to 24h of refrigeration and it was 30% higher than in natura semen at 12, 18 and 24h. Cell membrane integrity was maintained in fresh semen up to 6h, and it was 15-20% higher than in natura semen from 6 to 48h (p < 0.05). The use of CF-HBSS as diluent in the ratio 1:3 is recommended for semen refrigeration of lebranche mullet. Editora da Universidade Estadual de Maringá2018-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioncoleta de material in situ; análise laboratorialapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/3956310.4025/actascianimsci.v40i1.39563Acta Scientiarum. Animal Sciences; Vol 40 (2018): Publicação Contínua; e39563Acta Scientiarum. Animal Sciences; v. 40 (2018): Publicação Contínua; e395631807-86721806-2636reponame:Acta Scientiarum. Animal Sciences (Online)instname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/39563/pdfCopyright (c) 2018 Acta Scientiarum. Animal Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessMagnotti, Caio Cesar FrançaCastro, Jorgélia de Jesus PintoPedrotti, Fabiola SantiagoSterzelecki, Fábio CarneiroSanches, Eduardo GomesCerqueira, Vinicius Ronzani2019-07-17T08:31:19Zoai:periodicos.uem.br/ojs:article/39563Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciAnimSci/oaiactaanim@uem.br||actaanim@uem.br|| rev.acta@gmail.com1807-86721806-2636opendoar:2019-07-17T08:31:19Acta Scientiarum. Animal Sciences (Online) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
title Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
spellingShingle Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
Magnotti, Caio Cesar França
gamete conservation
spermatozoa
aquaculture
marine fish farming
near threatened fish.
Magnotti, Caio Cesar França
gamete conservation
spermatozoa
aquaculture
marine fish farming
near threatened fish.
title_short Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
title_full Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
title_fullStr Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
title_full_unstemmed Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
title_sort Short-term storage of lebranche mullet Mugil liza (Valenciennes, 1836) semen in natura and diluted with CF-HBSS
author Magnotti, Caio Cesar França
author_facet Magnotti, Caio Cesar França
Magnotti, Caio Cesar França
Castro, Jorgélia de Jesus Pinto
Pedrotti, Fabiola Santiago
Sterzelecki, Fábio Carneiro
Sanches, Eduardo Gomes
Cerqueira, Vinicius Ronzani
Castro, Jorgélia de Jesus Pinto
Pedrotti, Fabiola Santiago
Sterzelecki, Fábio Carneiro
Sanches, Eduardo Gomes
Cerqueira, Vinicius Ronzani
author_role author
author2 Castro, Jorgélia de Jesus Pinto
Pedrotti, Fabiola Santiago
Sterzelecki, Fábio Carneiro
Sanches, Eduardo Gomes
Cerqueira, Vinicius Ronzani
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Magnotti, Caio Cesar França
Castro, Jorgélia de Jesus Pinto
Pedrotti, Fabiola Santiago
Sterzelecki, Fábio Carneiro
Sanches, Eduardo Gomes
Cerqueira, Vinicius Ronzani
dc.subject.por.fl_str_mv gamete conservation
spermatozoa
aquaculture
marine fish farming
near threatened fish.
topic gamete conservation
spermatozoa
aquaculture
marine fish farming
near threatened fish.
description  The present study aimed to evaluate the activation characteristics of the lebranche mullet spermatozoa in natura and diluted with CF-HBSS for 96h at 4±2°C. The semen was collected from eight wild fish in Florianópolis – SC (Brazil) (27°S) in May, during reproductive migration. Three pools of semen were divided into two treatments: in natura and diluted with CF-HBSS 1:3. The semen was activated with seawater (salinity of 34, pH 8.7 and 4±2°C) to determine: motility time, motility rate and sperm cell membrane integrity. Dilution with CF-HBSS 1:3 increased motility time of diluted semen (15-20% for 6, 12 and 18h). Motility rate was equal to fresh semen for up to 24h of refrigeration and it was 30% higher than in natura semen at 12, 18 and 24h. Cell membrane integrity was maintained in fresh semen up to 6h, and it was 15-20% higher than in natura semen from 6 to 48h (p < 0.05). The use of CF-HBSS as diluent in the ratio 1:3 is recommended for semen refrigeration of lebranche mullet. 
publishDate 2018
dc.date.none.fl_str_mv 2018-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
coleta de material in situ; análise laboratorial
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/39563
10.4025/actascianimsci.v40i1.39563
url https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/39563
identifier_str_mv 10.4025/actascianimsci.v40i1.39563
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/39563/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2018 Acta Scientiarum. Animal Sciences
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Acta Scientiarum. Animal Sciences
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá
publisher.none.fl_str_mv Editora da Universidade Estadual de Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Animal Sciences; Vol 40 (2018): Publicação Contínua; e39563
Acta Scientiarum. Animal Sciences; v. 40 (2018): Publicação Contínua; e39563
1807-8672
1806-2636
reponame:Acta Scientiarum. Animal Sciences (Online)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum. Animal Sciences (Online)
collection Acta Scientiarum. Animal Sciences (Online)
repository.name.fl_str_mv Acta Scientiarum. Animal Sciences (Online) - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv actaanim@uem.br||actaanim@uem.br|| rev.acta@gmail.com
_version_ 1822182860919930880
dc.identifier.doi.none.fl_str_mv 10.4025/actascianimsci.v40i1.39563

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