Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements

• Autor(a) principal: Cunha, Nayara Nery de Oliveira
• Data de Publicação: 2021
• Outros Autores: Junqueira, Marina Azevedo, Cosme-Silva, Leopoldo, Santos, Laís da Silveira Terra, de Oliveira, George Augusto Veloso, Moretti Neto, Rafael Tobias, Nogueira, Denismar Alves, Brigagão, Maísa Ribeiro Pereira Lima, Moretti, Ana Beatriz da Silveira
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
• Texto Completo: https://revista.uepb.edu.br/PBOCI/article/view/658
• topic: Biocompatible Materials; Dental Pulp; Dental Pulp Capping; Pulpotomy; Peroxidase

Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods.