Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
Texto Completo: | https://revista.uepb.edu.br/PBOCI/article/view/658 |
Resumo: | Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods. |
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Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate CementsBiocompatible MaterialsDental PulpDental Pulp CappingPulpotomyPeroxidaseObjective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods.EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA2021-11-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.uepb.edu.br/PBOCI/article/view/658Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 21 (2021); e0106Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 21 (2021); e01061983-46321519-0501reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)instname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBenghttps://revista.uepb.edu.br/PBOCI/article/view/658/353Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integradahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCunha, Nayara Nery de Oliveira Junqueira, Marina AzevedoCosme-Silva, Leopoldo Santos, Laís da Silveira Terra de Oliveira, George Augusto Veloso Moretti Neto, Rafael Tobias Nogueira, Denismar Alves Brigagão, Maísa Ribeiro Pereira Lima Moretti, Ana Beatriz da Silveira2021-11-12T20:46:01Zoai:ojs.pkp.sfu.ca:article/658Revistahttps://revista.uepb.edu.br/PBOCI/PUBhttps://revista.uepb.edu.br/PBOCI/oaipboci.editoria@gmail.com || alessandrouepb@gmail.com1983-46321519-0501opendoar:2021-11-12T20:46:01Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)false |
dc.title.none.fl_str_mv |
Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements |
title |
Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements |
spellingShingle |
Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements Cunha, Nayara Nery de Oliveira Biocompatible Materials Dental Pulp Dental Pulp Capping Pulpotomy Peroxidase |
title_short |
Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements |
title_full |
Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements |
title_fullStr |
Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements |
title_full_unstemmed |
Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements |
title_sort |
Expression of Matrix Metalloproteinases-8 and Myeloperoxidase in Pulp Tissue after Pulpotomy with Calcium Silicate Cements |
author |
Cunha, Nayara Nery de Oliveira |
author_facet |
Cunha, Nayara Nery de Oliveira Junqueira, Marina Azevedo Cosme-Silva, Leopoldo Santos, Laís da Silveira Terra de Oliveira, George Augusto Veloso Moretti Neto, Rafael Tobias Nogueira, Denismar Alves Brigagão, Maísa Ribeiro Pereira Lima Moretti, Ana Beatriz da Silveira |
author_role |
author |
author2 |
Junqueira, Marina Azevedo Cosme-Silva, Leopoldo Santos, Laís da Silveira Terra de Oliveira, George Augusto Veloso Moretti Neto, Rafael Tobias Nogueira, Denismar Alves Brigagão, Maísa Ribeiro Pereira Lima Moretti, Ana Beatriz da Silveira |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cunha, Nayara Nery de Oliveira Junqueira, Marina Azevedo Cosme-Silva, Leopoldo Santos, Laís da Silveira Terra de Oliveira, George Augusto Veloso Moretti Neto, Rafael Tobias Nogueira, Denismar Alves Brigagão, Maísa Ribeiro Pereira Lima Moretti, Ana Beatriz da Silveira |
dc.subject.por.fl_str_mv |
Biocompatible Materials Dental Pulp Dental Pulp Capping Pulpotomy Peroxidase |
topic |
Biocompatible Materials Dental Pulp Dental Pulp Capping Pulpotomy Peroxidase |
description |
Objective: To evaluate the pulp tissue of rat molars after pulpotomies with mineral trioxide aggregate (MTA), BiodentineTM (BDT) and calcium hydroxide (CH) mixed with sterile saline solution (24 hours, 72 hours, 7 days and 15 days), through correlating MPO activity with active neutrophils and MMP8 activity with tissue remodeling. Material and Methods: Thirty-eight Wistar rats were randomly distributed into groups (control, I (MTA gray), II (BDT), and III (CH)) and subdivided according to the study period of 24 hours, 72 hours, 7 days or 15 days after pulpotomy. MMP8 activity was assessed through fluorescence technique, and MPO activity was determined using the MPO assay. Results: A gradual decrease of MPO and MM8 activity occurred in the group MTA over the experimental periods (p<0.05). Groups BDT and CH exhibited an increase in the activity at 7 and 15 days (p<0.05). Conclusion: MTA demonstrated a decrease in the values of MPO e MMP8. BDT and CH showed high neutrophilic and collagenase activity over the experimental periods. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revista.uepb.edu.br/PBOCI/article/view/658 |
url |
https://revista.uepb.edu.br/PBOCI/article/view/658 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revista.uepb.edu.br/PBOCI/article/view/658/353 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integrada https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Pesquisa Brasileira em Odontopediatria e Clínica Integrada https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA |
publisher.none.fl_str_mv |
EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA |
dc.source.none.fl_str_mv |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 21 (2021); e0106 Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 21 (2021); e0106 1983-4632 1519-0501 reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) instname:Universidade Estadual da Paraíba (UEPB) instacron:UEPB |
instname_str |
Universidade Estadual da Paraíba (UEPB) |
instacron_str |
UEPB |
institution |
UEPB |
reponame_str |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
collection |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) |
repository.name.fl_str_mv |
Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB) |
repository.mail.fl_str_mv |
pboci.editoria@gmail.com || alessandrouepb@gmail.com |
_version_ |
1803387862371008512 |