Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence

Detalhes bibliográficos
Autor(a) principal: Nuraini, Prawati
Data de Publicação: 2022
Outros Autores: Pradopo, Seno, Pronorahardjo, Amirah Saraswati
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
Texto Completo: https://revista.uepb.edu.br/PBOCI/article/view/1085
Resumo: Objective: To study the adherence of Streptococcus mutans biofilm after induction with sucrose and xylitol. Material and Methods: Laboratory experimental study incorporating posttest-only control group design. S. mutans biofilm was generated for 24 hours at a temperature of 37°C using BHIB with 5% sucrose and BHIB with 1% xylitol. An adherence assay was conducted in accordance with the method applied previously. The quantity of adhered bacteria was measured by means of a spectrophotometer at 570 nm. The data were presented as mean and standard deviation. Results: A biofilm induced with sucrose has a higher adherence level (0.9294 ± 0.0431) compared with one induced with xylitol (0.5095  ±  0.0392). Sucrose induces adherence levels by increasing glucan binding protein and glucosyltransferase of the bacteria, whereas xylitol will inhibit the glycolysis process of the bacteria. Conclusion: The adherence of sucrose-induced S. mutans biofilm is higher than that of xylitol-induced S. mutans biofilm.
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spelling Sucrose and Xylitol-Induced Streptococcus mutans Biofilm AdherenceDental PlaqueStreptococcus mutansSucroseXylitolObjective: To study the adherence of Streptococcus mutans biofilm after induction with sucrose and xylitol. Material and Methods: Laboratory experimental study incorporating posttest-only control group design. S. mutans biofilm was generated for 24 hours at a temperature of 37°C using BHIB with 5% sucrose and BHIB with 1% xylitol. An adherence assay was conducted in accordance with the method applied previously. The quantity of adhered bacteria was measured by means of a spectrophotometer at 570 nm. The data were presented as mean and standard deviation. Results: A biofilm induced with sucrose has a higher adherence level (0.9294 ± 0.0431) compared with one induced with xylitol (0.5095  ±  0.0392). Sucrose induces adherence levels by increasing glucan binding protein and glucosyltransferase of the bacteria, whereas xylitol will inhibit the glycolysis process of the bacteria. Conclusion: The adherence of sucrose-induced S. mutans biofilm is higher than that of xylitol-induced S. mutans biofilm.EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA2022-03-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.uepb.edu.br/PBOCI/article/view/1085Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 20 (2020); e4823 Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 20 (2020); e4823 1983-46321519-0501reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)instname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBenghttps://revista.uepb.edu.br/PBOCI/article/view/1085/801Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integradahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessNuraini, Prawati Pradopo, Seno Pronorahardjo, Amirah Saraswati 2022-03-18T03:32:34Zoai:ojs.pkp.sfu.ca:article/1085Revistahttps://revista.uepb.edu.br/PBOCI/PUBhttps://revista.uepb.edu.br/PBOCI/oaipboci.editoria@gmail.com || alessandrouepb@gmail.com1983-46321519-0501opendoar:2022-03-18T03:32:34Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)false
dc.title.none.fl_str_mv Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence
title Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence
spellingShingle Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence
Nuraini, Prawati
Dental Plaque
Streptococcus mutans
Sucrose
Xylitol
title_short Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence
title_full Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence
title_fullStr Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence
title_full_unstemmed Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence
title_sort Sucrose and Xylitol-Induced Streptococcus mutans Biofilm Adherence
author Nuraini, Prawati
author_facet Nuraini, Prawati
Pradopo, Seno
Pronorahardjo, Amirah Saraswati
author_role author
author2 Pradopo, Seno
Pronorahardjo, Amirah Saraswati
author2_role author
author
dc.contributor.author.fl_str_mv Nuraini, Prawati
Pradopo, Seno
Pronorahardjo, Amirah Saraswati
dc.subject.por.fl_str_mv Dental Plaque
Streptococcus mutans
Sucrose
Xylitol
topic Dental Plaque
Streptococcus mutans
Sucrose
Xylitol
description Objective: To study the adherence of Streptococcus mutans biofilm after induction with sucrose and xylitol. Material and Methods: Laboratory experimental study incorporating posttest-only control group design. S. mutans biofilm was generated for 24 hours at a temperature of 37°C using BHIB with 5% sucrose and BHIB with 1% xylitol. An adherence assay was conducted in accordance with the method applied previously. The quantity of adhered bacteria was measured by means of a spectrophotometer at 570 nm. The data were presented as mean and standard deviation. Results: A biofilm induced with sucrose has a higher adherence level (0.9294 ± 0.0431) compared with one induced with xylitol (0.5095  ±  0.0392). Sucrose induces adherence levels by increasing glucan binding protein and glucosyltransferase of the bacteria, whereas xylitol will inhibit the glycolysis process of the bacteria. Conclusion: The adherence of sucrose-induced S. mutans biofilm is higher than that of xylitol-induced S. mutans biofilm.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revista.uepb.edu.br/PBOCI/article/view/1085
url https://revista.uepb.edu.br/PBOCI/article/view/1085
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revista.uepb.edu.br/PBOCI/article/view/1085/801
dc.rights.driver.fl_str_mv Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integrada
https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Pesquisa Brasileira em Odontopediatria e Clínica Integrada
https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA
publisher.none.fl_str_mv EDUEPB - EDITORA DA UNIVERSIDADE ESTADUAL DA PARAÍBA
dc.source.none.fl_str_mv Pesquisa Brasileira em Odontopediatria e Clínica Integrada; Vol. 20 (2020); e4823
Pesquisa Brasileira em Odontopediatria e Clínica Integrada; v. 20 (2020); e4823
1983-4632
1519-0501
reponame:Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
instname:Universidade Estadual da Paraíba (UEPB)
instacron:UEPB
instname_str Universidade Estadual da Paraíba (UEPB)
instacron_str UEPB
institution UEPB
reponame_str Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
collection Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online)
repository.name.fl_str_mv Pesquisa Brasileira em Odontopediatria e Clínica Integrada (Online) - Universidade Estadual da Paraíba (UEPB)
repository.mail.fl_str_mv pboci.editoria@gmail.com || alessandrouepb@gmail.com
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