Calcium binding to S. mutans grown in the presence or absence of sucrose

Detalhes bibliográficos
Autor(a) principal: Leitão,Tarcísio Jorge
Data de Publicação: 2012
Outros Autores: Tenuta,Livia Maria Andaló, Ishi,Guilherme, Cury,Jaime Aparecido
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Oral Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000200003
Resumo: Sucrose is the most cariogenic dietary carbohydrate because it is a substrate for insoluble extracellular polysaccharide (IEPS) production in dental biofilms, which can proportionally decrease bacterial density and, consequently, the number of biofilm calcium (Ca) binding sites. Ca bound to bacterial cell walls can be released into the biofilm fluid during a cariogenic challenge, reducing the driving force for mineral dissolution provoked by the pH drop. Thus, we investigated the effect of an IEPS-rich extracellular matrix on bacterial Ca binding after treatment with Ca solutions. Streptococcus mutans Ingbritt 1600 was cultivated in culture broths supplemented with 1.0% sucrose or 0.5% glucose + 0.5% fructose. The IEPS concentration in bacterial pellets was determined after alkaline extraction. Bacterial pellets were treated with 1 mM or 10 mM Ca++ solutions at 37ºC for 10 to 60 min. Ca binding to bacterial pellets, determined after acid extraction using the Arsenazo III reagent, was fast and concentration dependent. Although the IEPS concentration was approximately ten times higher in bacterial pellets cultivated in sucrose as compared to its monossaccharides, bound Ca concentration after Ca treatment was similar in both conditions. These results suggest that IEPS may not influence the amount of Ca bound to reservoirs of dental biofilms.
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spelling Calcium binding to S. mutans grown in the presence or absence of sucroseSucroseStreptococcus mutansPolysaccharidesCalciumDental CariesSucrose is the most cariogenic dietary carbohydrate because it is a substrate for insoluble extracellular polysaccharide (IEPS) production in dental biofilms, which can proportionally decrease bacterial density and, consequently, the number of biofilm calcium (Ca) binding sites. Ca bound to bacterial cell walls can be released into the biofilm fluid during a cariogenic challenge, reducing the driving force for mineral dissolution provoked by the pH drop. Thus, we investigated the effect of an IEPS-rich extracellular matrix on bacterial Ca binding after treatment with Ca solutions. Streptococcus mutans Ingbritt 1600 was cultivated in culture broths supplemented with 1.0% sucrose or 0.5% glucose + 0.5% fructose. The IEPS concentration in bacterial pellets was determined after alkaline extraction. Bacterial pellets were treated with 1 mM or 10 mM Ca++ solutions at 37ºC for 10 to 60 min. Ca binding to bacterial pellets, determined after acid extraction using the Arsenazo III reagent, was fast and concentration dependent. Although the IEPS concentration was approximately ten times higher in bacterial pellets cultivated in sucrose as compared to its monossaccharides, bound Ca concentration after Ca treatment was similar in both conditions. These results suggest that IEPS may not influence the amount of Ca bound to reservoirs of dental biofilms.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000200003Brazilian Oral Research v.26 n.2 2012reponame:Brazilian Oral Researchinstname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)instacron:SBPQO10.1590/S1806-83242012000200003info:eu-repo/semantics/openAccessLeitão,Tarcísio JorgeTenuta,Livia Maria AndalóIshi,GuilhermeCury,Jaime Aparecidoeng2012-04-10T00:00:00Zoai:scielo:S1806-83242012000200003Revistahttps://www.scielo.br/j/bor/https://old.scielo.br/oai/scielo-oai.phppob@edu.usp.br||bor@sbpqo.org.br1807-31071806-8324opendoar:2012-04-10T00:00Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)false
dc.title.none.fl_str_mv Calcium binding to S. mutans grown in the presence or absence of sucrose
title Calcium binding to S. mutans grown in the presence or absence of sucrose
spellingShingle Calcium binding to S. mutans grown in the presence or absence of sucrose
Leitão,Tarcísio Jorge
Sucrose
Streptococcus mutans
Polysaccharides
Calcium
Dental Caries
title_short Calcium binding to S. mutans grown in the presence or absence of sucrose
title_full Calcium binding to S. mutans grown in the presence or absence of sucrose
title_fullStr Calcium binding to S. mutans grown in the presence or absence of sucrose
title_full_unstemmed Calcium binding to S. mutans grown in the presence or absence of sucrose
title_sort Calcium binding to S. mutans grown in the presence or absence of sucrose
author Leitão,Tarcísio Jorge
author_facet Leitão,Tarcísio Jorge
Tenuta,Livia Maria Andaló
Ishi,Guilherme
Cury,Jaime Aparecido
author_role author
author2 Tenuta,Livia Maria Andaló
Ishi,Guilherme
Cury,Jaime Aparecido
author2_role author
author
author
dc.contributor.author.fl_str_mv Leitão,Tarcísio Jorge
Tenuta,Livia Maria Andaló
Ishi,Guilherme
Cury,Jaime Aparecido
dc.subject.por.fl_str_mv Sucrose
Streptococcus mutans
Polysaccharides
Calcium
Dental Caries
topic Sucrose
Streptococcus mutans
Polysaccharides
Calcium
Dental Caries
description Sucrose is the most cariogenic dietary carbohydrate because it is a substrate for insoluble extracellular polysaccharide (IEPS) production in dental biofilms, which can proportionally decrease bacterial density and, consequently, the number of biofilm calcium (Ca) binding sites. Ca bound to bacterial cell walls can be released into the biofilm fluid during a cariogenic challenge, reducing the driving force for mineral dissolution provoked by the pH drop. Thus, we investigated the effect of an IEPS-rich extracellular matrix on bacterial Ca binding after treatment with Ca solutions. Streptococcus mutans Ingbritt 1600 was cultivated in culture broths supplemented with 1.0% sucrose or 0.5% glucose + 0.5% fructose. The IEPS concentration in bacterial pellets was determined after alkaline extraction. Bacterial pellets were treated with 1 mM or 10 mM Ca++ solutions at 37ºC for 10 to 60 min. Ca binding to bacterial pellets, determined after acid extraction using the Arsenazo III reagent, was fast and concentration dependent. Although the IEPS concentration was approximately ten times higher in bacterial pellets cultivated in sucrose as compared to its monossaccharides, bound Ca concentration after Ca treatment was similar in both conditions. These results suggest that IEPS may not influence the amount of Ca bound to reservoirs of dental biofilms.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1806-83242012000200003
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv Brazilian Oral Research v.26 n.2 2012
reponame:Brazilian Oral Research
instname:Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
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repository.name.fl_str_mv Brazilian Oral Research - Sociedade Brasileira de Pesquisa Odontológica (SBPqO)
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