Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPB |
Texto Completo: | http://tede.bc.uepb.edu.br/jspui/handle/tede/4054 |
Resumo: | The genus Croton, one of the largest in the Euphorbiaceae family, has aromatic species that produce essential oils that are characterized by their biological activities: anti-inflammatory, antioxidant, antimicrobial, cytotoxic, among others. The objective of this work was to investigate the chemical composition and antibacterial activity of the essential oils of the leaves from Croton urticifolius (OCU) and Croton adamantinus (OCA) collected in the municipality from Areia - PB. The essential oils from the fresh leaves of the Croton species were extracted by hydrodistillation in a Clevenger type apparatus and analyzed by gas chromatography coupled with mass spectrometry (GC/MS) to identify the chemical composition. The analysis by GC/MS allowed the identification of 22 compounds (95.0%), with the majority being sesquiterpenoids. The main compounds for C. urticifolius oil were α pinene (21.70%), bicyclogermacrene (21.46%) and (E) -cariophylene (17.34%), and from C. adamantinus oil were bicyclogermacrene (36.47%), γ-amorphene (22.91%) and (E) - cariophylene (9.22%). The antibacterial activity of the oils was tested against the strains Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 8739), using the Minimum Inhibitory Concentration (MIC) method in 96-well plates (microdilution). OCU showed weak activity against E. coli, with MIC of 512 μg.mL-1 . While the OCA showed no activity against any tested strain. The modulating effect of the oils was evaluated against the resistant S. aureus multidroga strain (UFPEDA 802) and the results obtained demonstrate that the association for OCU and OCA with the antibiotic Ciprofloxacino is positive, since it leads to the potentiation of the antibiotic effect. It is necessary to obtain more concrete data for the safe use of these combinations, paying attention to the existence of some type of existing toxicity reaction related to the herbal medicine and to understand the resistance mechanisms acquired by the microorganism. |
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Torres, Maria da Conceição de Menezes03888814471Teles, Yanna Carolina Ferreira06478502490Silva, Márcia Vanusa da6127713447270062995430Barbosa, Antonio Junior Costa2022-01-13T13:34:42Z2021-02-10BARBOSA, Antonio Junior Costa. Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE). 2021. 70f. Dissertação (Programa de Pós-Graduação em Química - PPGQ) - Universidade Estadual da Paraíba, Campina Grande, 2021.http://tede.bc.uepb.edu.br/jspui/handle/tede/4054The genus Croton, one of the largest in the Euphorbiaceae family, has aromatic species that produce essential oils that are characterized by their biological activities: anti-inflammatory, antioxidant, antimicrobial, cytotoxic, among others. The objective of this work was to investigate the chemical composition and antibacterial activity of the essential oils of the leaves from Croton urticifolius (OCU) and Croton adamantinus (OCA) collected in the municipality from Areia - PB. The essential oils from the fresh leaves of the Croton species were extracted by hydrodistillation in a Clevenger type apparatus and analyzed by gas chromatography coupled with mass spectrometry (GC/MS) to identify the chemical composition. The analysis by GC/MS allowed the identification of 22 compounds (95.0%), with the majority being sesquiterpenoids. The main compounds for C. urticifolius oil were α pinene (21.70%), bicyclogermacrene (21.46%) and (E) -cariophylene (17.34%), and from C. adamantinus oil were bicyclogermacrene (36.47%), γ-amorphene (22.91%) and (E) - cariophylene (9.22%). The antibacterial activity of the oils was tested against the strains Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 8739), using the Minimum Inhibitory Concentration (MIC) method in 96-well plates (microdilution). OCU showed weak activity against E. coli, with MIC of 512 μg.mL-1 . While the OCA showed no activity against any tested strain. The modulating effect of the oils was evaluated against the resistant S. aureus multidroga strain (UFPEDA 802) and the results obtained demonstrate that the association for OCU and OCA with the antibiotic Ciprofloxacino is positive, since it leads to the potentiation of the antibiotic effect. It is necessary to obtain more concrete data for the safe use of these combinations, paying attention to the existence of some type of existing toxicity reaction related to the herbal medicine and to understand the resistance mechanisms acquired by the microorganism.O gênero Croton, um dos maiores da família Euphorbiaceae, possui espécies aromáticas produtoras de óleos essenciais que são caracterizadas por apresentarem atividades biológicas: anti-inflamatória, antioxidante, antimicrobiana, citotóxica, entre outras. O objetivo deste trabalho foi investigar a composição química e a atividade antibacteriana dos óleos essenciais das folhas de Croton urticifolius (OCU) e Croton adamantinus (OCA) coletadas no município de Areia – PB. Os óleos essenciais das folhas frescas das espécies de Croton foram extraídos por hidrodestilação em aparelho do tipo Clevenger e analisados por cromatografia gasosa acoplada à espectrometria de massas (CG/EM) para identificar a composição química. A análise por CG/EM permitiu a identificação de 22 compostos (95,0%), com a sua maioria sendo sesquiterpenoides. Os principais compostos para o óleo de C. urticifolius foram α pineno (21,70%), biciclogermacreno (21,46%) e (E)-cariofileno (17,34%), e para o óleo de C. adamantinus foram o biciclogermacreno (36,47%), γ-amorfeno (22,91%) e (E)-cariofileno (9,22%). A atividade antibacteriana dos óleos foi testada frente às cepas Staphylococcus aureus (ATCC 6538) e Escherichia coli (ATCC 8739), pelo método de Concentração Inibitória Mínima (CIM) em placas de 96 poços (microdiluição). O OCU apresentou atividade fraca frente à E. coli, com CIM de 512 μg.mL-1 . Enquanto o OCA não apresentou atividade frente a nenhuma cepa testada. O efeito modulador dos óleos foi avaliado frente à cepa de S. aureus multidroga resistente (UFPEDA 802) e os resultados obtidos demonstram que a associação para o OCU e para o OCA com o antibiótico Ciprofloxacino são positivas, pois leva à potencialização do efeito antibiótico. É necessário obter dados mais concretos para o uso seguro dessas combinações, atentando-se para a existência de algum tipo de reação de toxicidade existente relacionada ao fitoterápico e entender os mecanismos de resistência adquiridos pelo microrganismo.Submitted by Jean Medeiros (jeanletras@uepb.edu.br) on 2021-09-09T12:20:56Z No. of bitstreams: 1 PDF - Antonio Junior Costa Barbosa.pdf: 2445999 bytes, checksum: 0877654dc1d22968f916c288e4f865e2 (MD5)Approved for entry into archive by Secta BC (secta.csu.bc@uepb.edu.br) on 2022-01-13T13:34:22Z (GMT) No. of bitstreams: 1 PDF - Antonio Junior Costa Barbosa.pdf: 2445999 bytes, checksum: 0877654dc1d22968f916c288e4f865e2 (MD5)Made available in DSpace on 2022-01-13T13:34:42Z (GMT). No. of bitstreams: 1 PDF - Antonio Junior Costa Barbosa.pdf: 2445999 bytes, checksum: 0877654dc1d22968f916c288e4f865e2 (MD5) Previous issue date: 2021-02-10application/pdfporUniversidade Estadual da ParaíbaPrograma de Pós-Graduação em Química - PPGQUEPBBrasilPró-Reitoria de Pós-Graduação e Pesquisa - PRPGPMarmeleiroHidrodestilaçãoQuímica medicinalAtividade antibacterianaHydrodistillationCIENCIAS EXATAS E DA TERRA::QUIMICAComposição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-79317878423444719666006006005248714503811102781571700325303117195info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPBinstname:Universidade Estadual da Paraíba (UEPB)instacron:UEPBORIGINALPDF - Antonio Junior Costa Barbosa.pdfPDF - Antonio Junior Costa Barbosa.pdfapplication/pdf2445999http://tede.bc.uepb.edu.br/jspui/bitstream/tede/4054/2/PDF+-+Antonio+Junior+Costa+Barbosa.pdf0877654dc1d22968f916c288e4f865e2MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81960http://tede.bc.uepb.edu.br/jspui/bitstream/tede/4054/1/license.txt6052ae61e77222b2086e666b7ae213ceMD51tede/40542022-01-13 10:34:42.11oai:tede.bc.uepb.edu.br:tede/4054TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlIApFc3RhZHVhbCBkYSBQYXJhw61iYSBvIGRpcmVpdG8gbsOjby1leGNsdXNpdm8gZGUgcmVwcm9kdXppciwgIHRyYWR1emlyIChjb25mb3JtZSBkZWZpbmlkbyBhYmFpeG8pLCBlL291IApkaXN0cmlidWlyIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0csO0bmljbyBlIAplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVFUEIgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyAKcGFyYSBxdWFscXVlciBtZWlvIG91IGZvcm1hdG8gcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBhIFVFUEIgcG9kZSBtYW50ZXIgbWFpcyBkZSB1bWEgY8OzcGlhIGRhIHN1YSB0ZXNlIG91IApkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrdXAgZSBwcmVzZXJ2YcOnw6NvLgoKVm9jw6ogZGVjbGFyYSBxdWUgYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIMOpIG9yaWdpbmFsIGUgcXVlIHZvY8OqIHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIApuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldSAKY29uaGVjaW1lbnRvLCBpbmZyaW5nZSBkaXJlaXRvcyBhdXRvcmFpcyBkZSBuaW5ndcOpbS4KCkNhc28gYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIGNvbnRlbmhhIG1hdGVyaWFsIHF1ZSB2b2PDqiBuw6NvIHBvc3N1aSBhIHRpdHVsYXJpZGFkZSBkb3MgZGlyZWl0b3MgYXV0b3JhaXMsIHZvY8OqIApkZWNsYXJhIHF1ZSBvYnRldmUgYSBwZXJtaXNzw6NvIGlycmVzdHJpdGEgZG8gZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhcmEgY29uY2VkZXIgw6AgVUVQQiAKb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIAppZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250ZcO6ZG8gZGEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFRFU0UgT1UgRElTU0VSVEHDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSAKQVBPSU8gREUgVU1BIEFHw4pOQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PIFFVRSBOw4NPIFNFSkEgQSBVRVBCLCAKVk9Dw4ogREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklTw4NPIENPTU8gVEFNQsOJTSBBUyAKREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRVBCIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUgKHMpIG91IG8ocykgbm9tZShzKSBkbyhzKSAKZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSB0ZXNlIG91IGRpc3NlcnRhw6fDo28sIGUgbsOjbyBmYXLDoSBxdWFscXVlciBhbHRlcmHDp8OjbywgYWzDqW0gZGFxdWVsYXMgCmNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7Dp2EuCg==Biblioteca Digital de Teses e Dissertaçõeshttp://tede.bc.uepb.edu.br/jspui/PUBhttp://tede.bc.uepb.edu.br/oai/requestbc@uepb.edu.br||opendoar:2022-01-13T13:34:42Biblioteca Digital de Teses e Dissertações da UEPB - Universidade Estadual da Paraíba (UEPB)false |
dc.title.por.fl_str_mv |
Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE) |
title |
Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE) |
spellingShingle |
Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE) Barbosa, Antonio Junior Costa Marmeleiro Hidrodestilação Química medicinal Atividade antibacteriana Hydrodistillation CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE) |
title_full |
Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE) |
title_fullStr |
Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE) |
title_full_unstemmed |
Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE) |
title_sort |
Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE) |
author |
Barbosa, Antonio Junior Costa |
author_facet |
Barbosa, Antonio Junior Costa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Torres, Maria da Conceição de Menezes |
dc.contributor.advisor1ID.fl_str_mv |
03888814471 |
dc.contributor.referee1.fl_str_mv |
Teles, Yanna Carolina Ferreira |
dc.contributor.referee1ID.fl_str_mv |
06478502490 |
dc.contributor.referee2.fl_str_mv |
Silva, Márcia Vanusa da |
dc.contributor.referee2ID.fl_str_mv |
61277134472 |
dc.contributor.authorID.fl_str_mv |
70062995430 |
dc.contributor.author.fl_str_mv |
Barbosa, Antonio Junior Costa |
contributor_str_mv |
Torres, Maria da Conceição de Menezes Teles, Yanna Carolina Ferreira Silva, Márcia Vanusa da |
dc.subject.por.fl_str_mv |
Marmeleiro Hidrodestilação Química medicinal Atividade antibacteriana |
topic |
Marmeleiro Hidrodestilação Química medicinal Atividade antibacteriana Hydrodistillation CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Hydrodistillation |
dc.subject.cnpq.fl_str_mv |
CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The genus Croton, one of the largest in the Euphorbiaceae family, has aromatic species that produce essential oils that are characterized by their biological activities: anti-inflammatory, antioxidant, antimicrobial, cytotoxic, among others. The objective of this work was to investigate the chemical composition and antibacterial activity of the essential oils of the leaves from Croton urticifolius (OCU) and Croton adamantinus (OCA) collected in the municipality from Areia - PB. The essential oils from the fresh leaves of the Croton species were extracted by hydrodistillation in a Clevenger type apparatus and analyzed by gas chromatography coupled with mass spectrometry (GC/MS) to identify the chemical composition. The analysis by GC/MS allowed the identification of 22 compounds (95.0%), with the majority being sesquiterpenoids. The main compounds for C. urticifolius oil were α pinene (21.70%), bicyclogermacrene (21.46%) and (E) -cariophylene (17.34%), and from C. adamantinus oil were bicyclogermacrene (36.47%), γ-amorphene (22.91%) and (E) - cariophylene (9.22%). The antibacterial activity of the oils was tested against the strains Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 8739), using the Minimum Inhibitory Concentration (MIC) method in 96-well plates (microdilution). OCU showed weak activity against E. coli, with MIC of 512 μg.mL-1 . While the OCA showed no activity against any tested strain. The modulating effect of the oils was evaluated against the resistant S. aureus multidroga strain (UFPEDA 802) and the results obtained demonstrate that the association for OCU and OCA with the antibiotic Ciprofloxacino is positive, since it leads to the potentiation of the antibiotic effect. It is necessary to obtain more concrete data for the safe use of these combinations, paying attention to the existence of some type of existing toxicity reaction related to the herbal medicine and to understand the resistance mechanisms acquired by the microorganism. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-02-10 |
dc.date.accessioned.fl_str_mv |
2022-01-13T13:34:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARBOSA, Antonio Junior Costa. Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE). 2021. 70f. Dissertação (Programa de Pós-Graduação em Química - PPGQ) - Universidade Estadual da Paraíba, Campina Grande, 2021. |
dc.identifier.uri.fl_str_mv |
http://tede.bc.uepb.edu.br/jspui/handle/tede/4054 |
identifier_str_mv |
BARBOSA, Antonio Junior Costa. Composição química e atividade antibacteriana dos óleos essenciais de Croton urticifolius LAM. e Croton adamantinus MÜLL. ARG. (EUPHORBIACEAE). 2021. 70f. Dissertação (Programa de Pós-Graduação em Química - PPGQ) - Universidade Estadual da Paraíba, Campina Grande, 2021. |
url |
http://tede.bc.uepb.edu.br/jspui/handle/tede/4054 |
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por |
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por |
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600 600 600 |
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1571700325303117195 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Estadual da Paraíba |
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UEPB |
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Brasil |
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Universidade Estadual da Paraíba |
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