AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS

Detalhes bibliográficos
Autor(a) principal: Silveira, Camila Maggi Maia
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEPG
Texto Completo: http://tede2.uepg.br/jspui/handle/prefix/1749
Resumo: The purpose of this study was evaluate the biocompatibility of four root canal sealers, Epiphany, AH Plus, Pulp Canal Sealer, Sealapex. Sterile polyethylene tubes containing freshly mixed test materials were inserted into the connective tissues of the sixty rats. The animals were divided into four groups. After anesthesia, the dorsal skin was shaved, disinfected, incisioned and two pocket were carefully prepared by blunt dissection and each animal received two implantations in opposite sides, one containing the material nd another as control (empty polyethylene tube). After periods of 7, 15 and 30 days, the implants were removed with the surrounding tissue and immersed in 10% formalin solution for 48 hours. After fixing the tissue it was processed for paraffin embedding which were cut in serial sections with the microtome set at 4 μm, stained with hematoxylin and eosin and assessed by light microscopy. The descriptive analysis was conducted by a pathologist who was blinded to which group and experimental period the glass slides pertained to, following the criteria: A) the thickness of the fibrous capsule: thin, intermediate or thick; B) Connective tissue composition: fibrous or loose, necrosis, nerve, blood vessels; C) the severity of the inflammatory reaction: mild, moderate, severe; D) presence of giant cells. The results revealed that after a period of 7 days all the sealers induced moderate-to-severe inflammatory reaction and Sealapex presented mild inflammatory reaction. At the 15-day, Epiphany and AH Plus sealers conducted to moderate inflammatory reaction, Pulp Canal Sealer severe inflammatory reaction, and Sealapex had the same intensity. After 30 days of the implantation of the sealers, mild inflammatory reactions were observed for the Epiphany, AH Plus and Sealapex, and moderate inflammatory reaction for the Pulp Canal Sealer. The results of this study revealed that Sealapex presented lowest level of inflammatory reaction in all the observed periods, yet all the sealers induced acceptable biological compatibility. More extensive experiments will be necessary to fully predict the clinical behavior of the sealers in patients undergoing endodontic therapy.
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spelling Pilatti, Gibson LuizCPF:80126324972http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4791325U6Santos, Fábio André dosCPF:1443095684http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768285J0Heck, Alexandre RobertoCPF:43687830934http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4716716A6CPF:84558319904http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4137445Z1Silveira, Camila Maggi Maia2017-07-24T19:22:09Z2009-10-162017-07-24T19:22:09Z2008-02-27SILVEIRA, Camila Maggi Maia. Analysis of the biocompatibility of four endodontic sealers: study in the subcutaneous connective tissue of rats. 2008. 81 f. Dissertação (Mestrado em Clinica Integrada, Dentística Restauradora e Periodontia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2008.http://tede2.uepg.br/jspui/handle/prefix/1749The purpose of this study was evaluate the biocompatibility of four root canal sealers, Epiphany, AH Plus, Pulp Canal Sealer, Sealapex. Sterile polyethylene tubes containing freshly mixed test materials were inserted into the connective tissues of the sixty rats. The animals were divided into four groups. After anesthesia, the dorsal skin was shaved, disinfected, incisioned and two pocket were carefully prepared by blunt dissection and each animal received two implantations in opposite sides, one containing the material nd another as control (empty polyethylene tube). After periods of 7, 15 and 30 days, the implants were removed with the surrounding tissue and immersed in 10% formalin solution for 48 hours. After fixing the tissue it was processed for paraffin embedding which were cut in serial sections with the microtome set at 4 μm, stained with hematoxylin and eosin and assessed by light microscopy. The descriptive analysis was conducted by a pathologist who was blinded to which group and experimental period the glass slides pertained to, following the criteria: A) the thickness of the fibrous capsule: thin, intermediate or thick; B) Connective tissue composition: fibrous or loose, necrosis, nerve, blood vessels; C) the severity of the inflammatory reaction: mild, moderate, severe; D) presence of giant cells. The results revealed that after a period of 7 days all the sealers induced moderate-to-severe inflammatory reaction and Sealapex presented mild inflammatory reaction. At the 15-day, Epiphany and AH Plus sealers conducted to moderate inflammatory reaction, Pulp Canal Sealer severe inflammatory reaction, and Sealapex had the same intensity. After 30 days of the implantation of the sealers, mild inflammatory reactions were observed for the Epiphany, AH Plus and Sealapex, and moderate inflammatory reaction for the Pulp Canal Sealer. The results of this study revealed that Sealapex presented lowest level of inflammatory reaction in all the observed periods, yet all the sealers induced acceptable biological compatibility. More extensive experiments will be necessary to fully predict the clinical behavior of the sealers in patients undergoing endodontic therapy.O objetivo deste estudo foi avaliar a biocompatibilidade de quatro cimentos endodônticos: Epiphany, AH Plus, Pulp Canal Sealer EWT e Sealapex. Tubos de polietileno contendo os materiais prontamente manipulados foram introduzidos no tecido conjuntivo subcutâneo de 60 ratos. Os animais foram divididos em 4 grupos. Após anestesia, tricotomia, desinfecção, incisão e divulsão tecidual, cada animal recebeu dois implantes contralaterais: um implante contendo o material e um controle (tubo vazio). Após os períodos de sete, 15 e 30 dias os implantes foram removidos com margem de tecido e fixados em solução de formol a 10% por 48 horas. Após a fixação das amostras, procedeu-se a remoção dos tubos, inclusão em blocos de parafina e cortes seriados de 4μm, que foram corados com Hematoxilina e Eosina e examinados por microscopia óptica de luz comum. A análise descritiva das lâminas foi realizada por um Patologista, que desconhecia a que grupo e período experimental as lâminas pertenciam, seguindo os seguintes critérios; A) Espessura da cápsula: delgada, intermediária ou espessa; B) Constituição do tecido conjuntivo: fibroso ou frouxo; presença de necrose; feixes vásculo-nervosos; eventos vasculares; C) Severidade do infiltrado inflamatório: discreto, moderado ou intenso; D) Presença de células gigantes multinucleadas inflamatórias (CGMI). Os resultados mostraram que depois de sete dias todos os cimentos apresentaram uma resposta inflamatória de moderada a intensa e o Sealapex mostrou-se com resposta inflamatória discreta. Com 15 dias os cimentos Epiphany e AH Plus apresentaram resposta moderada; para o Pulp Canal Sealer EWT a resposta foi severa e o Sealapex manteve-se com infiltrado inflamatório discreto. No final do período de avaliação, 30 dias, as reações teciduais apresentaram-se discretas para os cimentos AH Plus, Epiphany, Sealapex e moderada para o Pulp Canal Sealer EWT. Os resultados deste estudo revelaram que o cimento Sealapex foi o que apresentou menor resposta inflamatória em todos os períodos, ainda que todos os cimentos tenham demonstrado compatibilidade biológica aceitável. Contudo mais experimentos serão necessários para o total conhecimento do comportamento clínico dos cimentos em dentes submetidos a terapia endodôntica.Made available in DSpace on 2017-07-24T19:22:09Z (GMT). No. of bitstreams: 1 CAMILAMAIA.pdf: 8374902 bytes, checksum: 212930917cb97231aed01c002e3154e7 (MD5) Previous issue date: 2008-02-27Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUNIVERSIDADE ESTADUAL DE PONTA GROSSAPrograma de Pós-Graduação em OdontologiaUEPGBRClinica Integrada, Dentística Restauradora e Periodontiabiocompatibilidadecimentos endodônticosimplantaçãoInflamaçãobiocompatibilityroot canal sealersimplantationinflammationCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOSAnalysis of the biocompatibility of four endodontic sealers: study in the subcutaneous connective tissue of ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPGinstname:Universidade Estadual de Ponta Grossa (UEPG)instacron:UEPGORIGINALCAMILAMAIA.pdfapplication/pdf8374902http://tede2.uepg.br/jspui/bitstream/prefix/1749/1/CAMILAMAIA.pdf212930917cb97231aed01c002e3154e7MD51prefix/17492017-07-24 16:22:09.488oai:tede2.uepg.br:prefix/1749Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.uepg.br/jspui/PUBhttp://tede2.uepg.br/oai/requestbicen@uepg.br||mv_fidelis@yahoo.com.bropendoar:2017-07-24T19:22:09Biblioteca Digital de Teses e Dissertações da UEPG - Universidade Estadual de Ponta Grossa (UEPG)false
dc.title.por.fl_str_mv AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS
dc.title.alternative.eng.fl_str_mv Analysis of the biocompatibility of four endodontic sealers: study in the subcutaneous connective tissue of rats
title AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS
spellingShingle AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS
Silveira, Camila Maggi Maia
biocompatibilidade
cimentos endodônticos
implantação
Inflamação
biocompatibility
root canal sealers
implantation
inflammation
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
title_short AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS
title_full AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS
title_fullStr AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS
title_full_unstemmed AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS
title_sort AVALIAÇÃO DA BIOCOMPATIBILIDADE DE 4 CIMENTOS ENDODÔNTICOS:ESTUDO EM TECIDO CONJUNTIVO SUBCUTÂNEO DE RATOS
author Silveira, Camila Maggi Maia
author_facet Silveira, Camila Maggi Maia
author_role author
dc.contributor.advisor1.fl_str_mv Pilatti, Gibson Luiz
dc.contributor.advisor1ID.fl_str_mv CPF:80126324972
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4791325U6
dc.contributor.referee1.fl_str_mv Santos, Fábio André dos
dc.contributor.referee1ID.fl_str_mv CPF:1443095684
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768285J0
dc.contributor.referee2.fl_str_mv Heck, Alexandre Roberto
dc.contributor.referee2ID.fl_str_mv CPF:43687830934
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4716716A6
dc.contributor.authorID.fl_str_mv CPF:84558319904
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4137445Z1
dc.contributor.author.fl_str_mv Silveira, Camila Maggi Maia
contributor_str_mv Pilatti, Gibson Luiz
Santos, Fábio André dos
Heck, Alexandre Roberto
dc.subject.por.fl_str_mv biocompatibilidade
cimentos endodônticos
implantação
Inflamação
topic biocompatibilidade
cimentos endodônticos
implantação
Inflamação
biocompatibility
root canal sealers
implantation
inflammation
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.eng.fl_str_mv biocompatibility
root canal sealers
implantation
inflammation
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
description The purpose of this study was evaluate the biocompatibility of four root canal sealers, Epiphany, AH Plus, Pulp Canal Sealer, Sealapex. Sterile polyethylene tubes containing freshly mixed test materials were inserted into the connective tissues of the sixty rats. The animals were divided into four groups. After anesthesia, the dorsal skin was shaved, disinfected, incisioned and two pocket were carefully prepared by blunt dissection and each animal received two implantations in opposite sides, one containing the material nd another as control (empty polyethylene tube). After periods of 7, 15 and 30 days, the implants were removed with the surrounding tissue and immersed in 10% formalin solution for 48 hours. After fixing the tissue it was processed for paraffin embedding which were cut in serial sections with the microtome set at 4 μm, stained with hematoxylin and eosin and assessed by light microscopy. The descriptive analysis was conducted by a pathologist who was blinded to which group and experimental period the glass slides pertained to, following the criteria: A) the thickness of the fibrous capsule: thin, intermediate or thick; B) Connective tissue composition: fibrous or loose, necrosis, nerve, blood vessels; C) the severity of the inflammatory reaction: mild, moderate, severe; D) presence of giant cells. The results revealed that after a period of 7 days all the sealers induced moderate-to-severe inflammatory reaction and Sealapex presented mild inflammatory reaction. At the 15-day, Epiphany and AH Plus sealers conducted to moderate inflammatory reaction, Pulp Canal Sealer severe inflammatory reaction, and Sealapex had the same intensity. After 30 days of the implantation of the sealers, mild inflammatory reactions were observed for the Epiphany, AH Plus and Sealapex, and moderate inflammatory reaction for the Pulp Canal Sealer. The results of this study revealed that Sealapex presented lowest level of inflammatory reaction in all the observed periods, yet all the sealers induced acceptable biological compatibility. More extensive experiments will be necessary to fully predict the clinical behavior of the sealers in patients undergoing endodontic therapy.
publishDate 2008
dc.date.issued.fl_str_mv 2008-02-27
dc.date.available.fl_str_mv 2009-10-16
2017-07-24T19:22:09Z
dc.date.accessioned.fl_str_mv 2017-07-24T19:22:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVEIRA, Camila Maggi Maia. Analysis of the biocompatibility of four endodontic sealers: study in the subcutaneous connective tissue of rats. 2008. 81 f. Dissertação (Mestrado em Clinica Integrada, Dentística Restauradora e Periodontia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2008.
dc.identifier.uri.fl_str_mv http://tede2.uepg.br/jspui/handle/prefix/1749
identifier_str_mv SILVEIRA, Camila Maggi Maia. Analysis of the biocompatibility of four endodontic sealers: study in the subcutaneous connective tissue of rats. 2008. 81 f. Dissertação (Mestrado em Clinica Integrada, Dentística Restauradora e Periodontia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2008.
url http://tede2.uepg.br/jspui/handle/prefix/1749
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UNIVERSIDADE ESTADUAL DE PONTA GROSSA
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Odontologia
dc.publisher.initials.fl_str_mv UEPG
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Clinica Integrada, Dentística Restauradora e Periodontia
publisher.none.fl_str_mv UNIVERSIDADE ESTADUAL DE PONTA GROSSA
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UEPG
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reponame_str Biblioteca Digital de Teses e Dissertações da UEPG
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