ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UEPG |
Texto Completo: | http://tede2.uepg.br/jspui/handle/prefix/976 |
Resumo: | The urinary tract infections (UTI) have high incidence in the population, mainly affecting women, the elderly and pregnant women. In most cases an UTI is caused by Enterobacteriaceae, especially Escherichia coli. The main antimicrobials used in the treatment of UTIs, are the quinolones and fluoroquinolones. Since its discovery in the 60s, such antimicrobials were improved and increasing its spectrum of activity, but also the micro-organisms has evolved to develop resistance to these drugs. The resistance comes down to two mechanisms: changes in targets of quinolones or decreased concentration of the drug within the cell. Quinolones are antimicrobial agents that target two enzymes involved in transcription and replication of bacterial DNA, DNA Gyrase, consisting of two subunits GyrA and two GyrB products of the genes gyrA and gyrB respectively and Topoisomerase IV, also composed of two ParC and two ParE subunits, encoded from genes parC and parE. Mutations in these genes can confer high-level resistance to these antimicrobials. Other mechanisms involve genes carried in plasmids such as qnr's, responsible for the production of proteins that protect the target enzymes of quinolones. The gene aac (6 ')-Ib-cr also present in plasmids can alter the chemical structure of certain fluoroquinolones interfering efficiency. The association of resistance to quinolones and β-lactams due to the presence of Extended Spectrum β-lactamases (ESBL) has been widely reported in the literature. The main objective of this study was to evaluate the patterns of resistance to quinolones and fluoroquinolones in 55 samples of uropathogenic E. coli collected in Curitiba, PR, Brazil. The resistance to nalidixic acid, norfloxacin, ciprofloxacin and ofloxacin was observed in 69.1% of the samples. Molecular analysis of the resistant strains showed mutations in gyrA (codons 83 and 87) and parC (codon 80). Also investigated was the presence of genes qnr's and aac (6 ')-Ib-cr and their association with ESBLs. Two of the 55 samples were positive for aac (6 ')-Ib-cr. The presence of this gene in Brazil was first reported in 2012 in Belo Horizonte, MG. No samples were positive for the qnr's genes. These results show a high incidence of resistance to quinolones and the ability to horizontally spread of acquired resistance between the bacterial strains. |
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Silveira, Rafael Bertoni daCPF:02452206903http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4760777E5Galvão, Carolina WeigertCPF:00534598900http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4760025Y8Rego, Fabiane Gomes de MoraesCPF:96169710934http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790687Z0Emilio, Henriettte Rosa de OliveiraCPF:26925492871http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767157T3CPF:85074888972http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4414298P7Freitas, Denis Leandro de2017-07-21T19:59:59Z2014-02-212017-07-21T19:59:59Z2013-03-27FREITAS, Denis Leandro de. ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA. 2013. 78 f. Dissertação (Mestrado em Biologia Evolutiva) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa Grossa, 2013.http://tede2.uepg.br/jspui/handle/prefix/976The urinary tract infections (UTI) have high incidence in the population, mainly affecting women, the elderly and pregnant women. In most cases an UTI is caused by Enterobacteriaceae, especially Escherichia coli. The main antimicrobials used in the treatment of UTIs, are the quinolones and fluoroquinolones. Since its discovery in the 60s, such antimicrobials were improved and increasing its spectrum of activity, but also the micro-organisms has evolved to develop resistance to these drugs. The resistance comes down to two mechanisms: changes in targets of quinolones or decreased concentration of the drug within the cell. Quinolones are antimicrobial agents that target two enzymes involved in transcription and replication of bacterial DNA, DNA Gyrase, consisting of two subunits GyrA and two GyrB products of the genes gyrA and gyrB respectively and Topoisomerase IV, also composed of two ParC and two ParE subunits, encoded from genes parC and parE. Mutations in these genes can confer high-level resistance to these antimicrobials. Other mechanisms involve genes carried in plasmids such as qnr's, responsible for the production of proteins that protect the target enzymes of quinolones. The gene aac (6 ')-Ib-cr also present in plasmids can alter the chemical structure of certain fluoroquinolones interfering efficiency. The association of resistance to quinolones and β-lactams due to the presence of Extended Spectrum β-lactamases (ESBL) has been widely reported in the literature. The main objective of this study was to evaluate the patterns of resistance to quinolones and fluoroquinolones in 55 samples of uropathogenic E. coli collected in Curitiba, PR, Brazil. The resistance to nalidixic acid, norfloxacin, ciprofloxacin and ofloxacin was observed in 69.1% of the samples. Molecular analysis of the resistant strains showed mutations in gyrA (codons 83 and 87) and parC (codon 80). Also investigated was the presence of genes qnr's and aac (6 ')-Ib-cr and their association with ESBLs. Two of the 55 samples were positive for aac (6 ')-Ib-cr. The presence of this gene in Brazil was first reported in 2012 in Belo Horizonte, MG. No samples were positive for the qnr's genes. These results show a high incidence of resistance to quinolones and the ability to horizontally spread of acquired resistance between the bacterial strains.As infecções do trato urinário (ITU) apresentam grande ocorrência na população, afetando principalmente mulheres, idosos e gestantes. Na maioria dos casos uma ITU é causada por enterobactérias, especialmente Escherichia coli. A principal escolha no tratamento das ITUs são as quinolonas e fluorquinolonas. Desde sua descoberta na década de 60, tais antimicrobianos foram aperfeiçoados aumentando seu espectro de ação, porém os micro-organismos também evoluíram desenvolvendo resistência a tais fármacos. A resistência se resume a alterações nos alvos das quinolonas e ou a diminuição da concentração da droga no interior da célula. Quinolonas são antimicrobianos que têm como alvo duas enzimas envolvidas na replicação e transcrição do DNA bacteriano, a DNA Girase, formada por duas subunidades GyrA e duas GyrB, produtos dos genes gyrA e gyrB respectivamente e a Topoisomerase IV, também composta de duas subunidades ParC e duas ParE, codificadas a partir dos genes parC e parE. Mutações nesses genes podem conferir alto nível de resistência a estes antimicrobianos. Outros mecanismos envolvem genes transportados em plasmídeos, como os qnr’s, responsáveis pela produção de proteínas capazes de proteger as enzimas alvo das quinolonas. O gene aac(6’)-ib-cr, também presente em plasmídeos, é capaz de alterar a estrutura química de certas fluorquinolonas interferindo na sua eficiência. A associação de resistência a quinolonas e β-lactâmicos devido à presença de β-lactamases de espectro ampliado (ESBLs), tem sido amplamente relatada na literatura. O principal objetivo desse trabalho foi avaliar os padrões de resistência a quinolonas e fluorquinolonas em 55 amostras de E. coli uropatogênica coletadas em Curitiba, PR. A resistência ao ácido nalidíxico, a norfloxacina, ciprofloxacina e ofloxacina, foi observada em 69,1% das amostras. A análise molecular das cepas resistentes revelou mutações em gyrA (códons 83 e 87) e em parC (códon 80). Também foi pesquisada a presença dos genes qnr’s e aac(6’)-ib-cr e a associação destes com ESBLs. Duas das 55 amostras foram positivas para aac(6’)-ib-cr. A presença desse gene no Brasil foi relatada pela primeira vez em 2012, em Belo Horizonte, MG. Nenhuma amostra foi positiva para os genes qnr’s. Estes resultados mostram uma elevada incidência de resistência às quinolonas e a capacidade de transferência horizontal de resistência adquirida entre as cepas bacterianas.Made available in DSpace on 2017-07-21T19:59:59Z (GMT). No. of bitstreams: 1 Denis Leandro Freitas.pdf: 2460536 bytes, checksum: 9c33d2a38cc2aba90b9f27c476e8d2c5 (MD5) Previous issue date: 2013-03-27Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paranáapplication/pdfporUNIVERSIDADE ESTADUAL DE PONTA GROSSAPrograma de Pós-Graduação em Ciências BiológicasUEPGBRBiologia Evolutivaresistênciaquinolonasmutaçãoaac(6’)-ib-crresistancequinolonesmutationaac (6 ')-Ib-crCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULARANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPGinstname:Universidade Estadual de Ponta Grossa (UEPG)instacron:UEPGORIGINALDenis Leandro Freitas.pdfapplication/pdf2460536http://tede2.uepg.br/jspui/bitstream/prefix/976/1/Denis%20Leandro%20Freitas.pdf9c33d2a38cc2aba90b9f27c476e8d2c5MD51prefix/9762017-07-21 16:59:59.321oai:tede2.uepg.br:prefix/976Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.uepg.br/jspui/PUBhttp://tede2.uepg.br/oai/requestbicen@uepg.br||mv_fidelis@yahoo.com.bropendoar:2017-07-21T19:59:59Biblioteca Digital de Teses e Dissertações da UEPG - Universidade Estadual de Ponta Grossa (UEPG)false |
dc.title.por.fl_str_mv |
ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA |
title |
ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA |
spellingShingle |
ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA Freitas, Denis Leandro de resistência quinolonas mutação aac(6’)-ib-cr resistance quinolones mutation aac (6 ')-Ib-cr CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
title_short |
ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA |
title_full |
ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA |
title_fullStr |
ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA |
title_full_unstemmed |
ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA |
title_sort |
ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA |
author |
Freitas, Denis Leandro de |
author_facet |
Freitas, Denis Leandro de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silveira, Rafael Bertoni da |
dc.contributor.advisor1ID.fl_str_mv |
CPF:02452206903 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4760777E5 |
dc.contributor.advisor-co1.fl_str_mv |
Galvão, Carolina Weigert |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:00534598900 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4760025Y8 |
dc.contributor.referee1.fl_str_mv |
Rego, Fabiane Gomes de Moraes |
dc.contributor.referee1ID.fl_str_mv |
CPF:96169710934 |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790687Z0 |
dc.contributor.referee2.fl_str_mv |
Emilio, Henriettte Rosa de Oliveira |
dc.contributor.referee2ID.fl_str_mv |
CPF:26925492871 |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4767157T3 |
dc.contributor.authorID.fl_str_mv |
CPF:85074888972 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4414298P7 |
dc.contributor.author.fl_str_mv |
Freitas, Denis Leandro de |
contributor_str_mv |
Silveira, Rafael Bertoni da Galvão, Carolina Weigert Rego, Fabiane Gomes de Moraes Emilio, Henriettte Rosa de Oliveira |
dc.subject.por.fl_str_mv |
resistência quinolonas mutação aac(6’)-ib-cr |
topic |
resistência quinolonas mutação aac(6’)-ib-cr resistance quinolones mutation aac (6 ')-Ib-cr CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
dc.subject.eng.fl_str_mv |
resistance quinolones mutation aac (6 ')-Ib-cr |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR |
description |
The urinary tract infections (UTI) have high incidence in the population, mainly affecting women, the elderly and pregnant women. In most cases an UTI is caused by Enterobacteriaceae, especially Escherichia coli. The main antimicrobials used in the treatment of UTIs, are the quinolones and fluoroquinolones. Since its discovery in the 60s, such antimicrobials were improved and increasing its spectrum of activity, but also the micro-organisms has evolved to develop resistance to these drugs. The resistance comes down to two mechanisms: changes in targets of quinolones or decreased concentration of the drug within the cell. Quinolones are antimicrobial agents that target two enzymes involved in transcription and replication of bacterial DNA, DNA Gyrase, consisting of two subunits GyrA and two GyrB products of the genes gyrA and gyrB respectively and Topoisomerase IV, also composed of two ParC and two ParE subunits, encoded from genes parC and parE. Mutations in these genes can confer high-level resistance to these antimicrobials. Other mechanisms involve genes carried in plasmids such as qnr's, responsible for the production of proteins that protect the target enzymes of quinolones. The gene aac (6 ')-Ib-cr also present in plasmids can alter the chemical structure of certain fluoroquinolones interfering efficiency. The association of resistance to quinolones and β-lactams due to the presence of Extended Spectrum β-lactamases (ESBL) has been widely reported in the literature. The main objective of this study was to evaluate the patterns of resistance to quinolones and fluoroquinolones in 55 samples of uropathogenic E. coli collected in Curitiba, PR, Brazil. The resistance to nalidixic acid, norfloxacin, ciprofloxacin and ofloxacin was observed in 69.1% of the samples. Molecular analysis of the resistant strains showed mutations in gyrA (codons 83 and 87) and parC (codon 80). Also investigated was the presence of genes qnr's and aac (6 ')-Ib-cr and their association with ESBLs. Two of the 55 samples were positive for aac (6 ')-Ib-cr. The presence of this gene in Brazil was first reported in 2012 in Belo Horizonte, MG. No samples were positive for the qnr's genes. These results show a high incidence of resistance to quinolones and the ability to horizontally spread of acquired resistance between the bacterial strains. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-03-27 |
dc.date.available.fl_str_mv |
2014-02-21 2017-07-21T19:59:59Z |
dc.date.accessioned.fl_str_mv |
2017-07-21T19:59:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FREITAS, Denis Leandro de. ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA. 2013. 78 f. Dissertação (Mestrado em Biologia Evolutiva) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa Grossa, 2013. |
dc.identifier.uri.fl_str_mv |
http://tede2.uepg.br/jspui/handle/prefix/976 |
identifier_str_mv |
FREITAS, Denis Leandro de. ANÁLISE MOLECULAR DE RESISTÊNCIA A QUINOLONAS E FLUORQUINOLONAS EM Escherichia coli UROPATOGÊNICA. 2013. 78 f. Dissertação (Mestrado em Biologia Evolutiva) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa Grossa, 2013. |
url |
http://tede2.uepg.br/jspui/handle/prefix/976 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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UNIVERSIDADE ESTADUAL DE PONTA GROSSA |
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Programa de Pós-Graduação em Ciências Biológicas |
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UEPG |
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BR |
dc.publisher.department.fl_str_mv |
Biologia Evolutiva |
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UNIVERSIDADE ESTADUAL DE PONTA GROSSA |
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Biblioteca Digital de Teses e Dissertações da UEPG |
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Biblioteca Digital de Teses e Dissertações da UEPG |
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