Avalição das propriedades físico-quimicas, de absorção percutânea e de biocompatibilidade de gel anestésico termosensível

• Autor(a) principal: Laufer Neto, José
• Data de Publicação: 2006
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UEPG
• Texto Completo: http://tede2.uepg.br/jspui/handle/prefix/1770
• topic: lidocaína; prilocaína; anestésicos tópicos; lidocaine; prilocaine; topical anesthetics; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

The no invasive anesthesia by application of an anesthetic gel in the interior of the periodontal pocket appears as an alternative in the periodontal treatment. The objective of this study was to manipulate, to test the physical-chemical properties, the percutaneous absorption of a gel thermosetting topical anesthetic lidocaine/prilocaine 5 Test) and evaluates its biocompatibility. Preformulation studies, microbiological and percutaneous absorption (PA) in vitro and in vivo, has been done. To evaluate aspects of biocompatibility 2 techniques had been done: vascular permeability (VP) and descriptive histological analyses (HA). In the PV, 60 rats and 3 periods of observation had been used (3, 6, 9 hours) and were evaluated,visually, for the area of the inflammation and for dye concentration applied, by spectrophotometric analysis, after mplantation of 0,1 mL of the substances: G1- Teste; G2-Emla; G3-Poloxamer; G4-Controle. In AH, 60 rats, were divided in 4 groups (in the same way cited before) with subcutaneous implantation of polyethylene tubes with substances had been used, in periods of 2, 5 and 15 days. The pharmacotechnical results had shown values of pH 7,71, density (gel 1,020),not occurring microorganisms growth (gram+, gram- and fungi) in the substance Test and showing a good viscosity, adjusted for use in oral cavity. AP did not show significant differences between the GI and GII, with p>0,05 (Mann-Whitney) for the experiments in vitro and in vivo. In the PV, G1 and G2 had bigger values (p< 0,05) than G3 and G4. The histological analysis did not show areas of necrosis, nor severe inflammatory response. It is concluded that the gel Test presents adequate properties and showed not to induce severe inflammatory response in the subcutaneous, showing biocompatibility when compared with the control group.