Avaliação de novos processos de limpeza para quantificação de pireno em amostra de mexilhão

• Autor(a) principal: Andrade, Priscila Mendonça de
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/15766
• topic: Mussels; clean-up; clay®K-10; HPA; pyrene; Mexilhão; clean-up; argila® K-10; HPA; pireno; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA

Polycyclic aromatic hydrocarbons (PAH) are a group of persistent environmental pollutants are molecules containing two or more condensed aromatic rings, little soluble in water and are present in the environment as a result of natural processes and human activities; in this way, the HPA can be found in various environmental matrices. In this work we used mussels in Búzios, Rio de Janeiro State. That body was selected due to the ease of accumulation of HPA in the tissues, due to the hydrophobic properties of HPA. This project aimed to study a new cleaning material able to minimize interfering compounds of the array. The 5 g Florisil® SPE cartridges, are conventionally used in the cleaning of samples of organisms, however for lower costs have been tested other adsorbent materials which could be effective in the removal of the present in the tissues of mussels interfering. Therefore, in step of cleaning known as clean-up, recovery of pyrene was studied after extraction by microwave through two different approaches; a) use of commercial SPE cartridges 5 g of Florisil®; b) cartridges filled with K-10 clay® bentonite commercial, simulating the commercial cartridges. Recovery and efficiency of cleaning procedures were tested and compared. The clean-up with K-10 clay presented a recovery of up to 77% of pyrene, being effective in removing compounds of cholesterols; confirming the efficiency of the chosen material for cleaning of the sample. The technique of gas chromatography and detection by mass spectrometry (GC-MS) was applied for identification and quantification of contaminant pyrene in the final extract