Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Bruno Leonardo Marroig de Freitas
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
Texto Completo: http://www.bdtd.uerj.br/handle/1/12363
Resumo: The cellular mechanisms involved in benign prostatic hyperplasia (BPH) are not well understood, and little is known about how proteoglycans are affected. Here we investigated the protein expression of stromal and cell surface proteoglycans in BPH. BPH samples were colected from open prostatectomy and transurethral resection of the prostate (TURP), while controls consisted of the transitional zone of normal prostates from young adults. We used primary antibodies against the proteoglycans syndecan-1, biglycan, and decorin. Immunolabeling was evaluated by determining the relative area of staining, or by using a score for staining intensity. The results showed that, in controls, syndecan-1 was mostly negative. In BPH, however, the labeling of this proteoglycan was intense and located mainly in acinar basal cells, but could extended into the secretory cells. As there were no differences in samples from open prostatectomy and TURP, these groups were combined into a BPH group. Syndecan-1 labeling area in the epithelium of BPH samples was nine times greater than that of controls (p<0,001), and there were no significant correlations between syndecan-1 labeling in BPH and prostate volume, PSA, or patient s age. Biglycan and decorin labeling were in the stroma exclusively, in control and BPH samples, and there were no significant differences in extent and intensity of the staining between these two groups. In conclusion, syndecan-1 immunolabeling in BPH is prominent and is located in the glandular epithelium exclusively, but intensity does not correlate with prostate size or PSA. Decorin and biglycan labeling, however, were unchanged in BPH.
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spelling Cardoso, Luiz Eduardo de Macedohttp://lattes.cnpq.br/3117933597142016Sampaio, Francisco José Barcelloshttp://lattes.cnpq.br/4426830285523015Costa, Waldemar Silvahttp://lattes.cnpq.br/6986274610951714Babinski, Marcio Antoniohttp://lattes.cnpq.br/7985386665524107Fortes, Marco Antonio Quesada Ribeirohttp://lattes.cnpq.br/9531672809534888http://lattes.cnpq.br/3575262832461071Ribeiro, Bruno Leonardo Marroig de Freitas2021-01-06T20:38:55Z2015-07-152011-06-30http://www.bdtd.uerj.br/handle/1/12363The cellular mechanisms involved in benign prostatic hyperplasia (BPH) are not well understood, and little is known about how proteoglycans are affected. Here we investigated the protein expression of stromal and cell surface proteoglycans in BPH. BPH samples were colected from open prostatectomy and transurethral resection of the prostate (TURP), while controls consisted of the transitional zone of normal prostates from young adults. We used primary antibodies against the proteoglycans syndecan-1, biglycan, and decorin. Immunolabeling was evaluated by determining the relative area of staining, or by using a score for staining intensity. The results showed that, in controls, syndecan-1 was mostly negative. In BPH, however, the labeling of this proteoglycan was intense and located mainly in acinar basal cells, but could extended into the secretory cells. As there were no differences in samples from open prostatectomy and TURP, these groups were combined into a BPH group. Syndecan-1 labeling area in the epithelium of BPH samples was nine times greater than that of controls (p<0,001), and there were no significant correlations between syndecan-1 labeling in BPH and prostate volume, PSA, or patient s age. Biglycan and decorin labeling were in the stroma exclusively, in control and BPH samples, and there were no significant differences in extent and intensity of the staining between these two groups. In conclusion, syndecan-1 immunolabeling in BPH is prominent and is located in the glandular epithelium exclusively, but intensity does not correlate with prostate size or PSA. Decorin and biglycan labeling, however, were unchanged in BPH.Os mecanismos celulares envolvidos na hiperplasia prostática benigna (HPB) não são bem compreendidos e pouco se sabe sobre como os proteoglicanos estão relacionados com esta condição. Neste estudo foi avaliada a expressão de proteoglicanos de superfície celular e do estroma na HPB. As amostras de próstata com HPB foram colhidas de pacientes submetidos à prostatectomia aberta e ressecção transuretral da próstata (RTUP), enquanto as amostras do grupo controle consistiram na zona de transição de próstatas normais de adultos jovens. Foram usados anticorpos primários anti-sindecan-1, anti-biglican e anti-decorin. A imunomarcação foi avaliada determinando-se a área relativa marcada pelo anticorpo, ou usando-se um escore atribuído à intensidade da coloração. Os resultados mostraram que, no grupo controle, a expressão do sindecan-1 foi mínima ou nula. Na HPB, no entanto, a imunomarcação deste proteoglicano foi intensa e localizada principalmente nas células basais do ácino prostático e com menor intensidade na superfície basolateral das células secretoras. Como não houve diferença entre as amostras obtidas através da prostatectomia aberta e da RTUP, esses grupos foram combinados em um único grupo HPB. A área marcada pelo anticorpo anti-sindecan-1 no epitélio das amostras de HPB foi nove vezes maior do que na próstata normal (p<0,001), e não houve correlações significativas entre a marcação do sindecan-1naHPB e o volume da próstata, o PSA ou a idade do paciente. Quanto ao biglican e ao decorin, a marcação foi exclusivamente no estroma, tanto no grupo controle quanto no grupo HPB, e não houve diferença significativa na extensão e intensidade da coloração entre estes dois grupos. Em conclusão, a imunomarcação do sindecan-1 na HPB é intensa e está localizada no epitélio glandular exclusivamente, mas a intensidade não se correlaciona com o tamanho da próstata ou PSA. A expressão do decorin e do biglican, no entanto, não foi alterada na HPB.Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:38:55Z No. of bitstreams: 1 DISSERTACAO_FINAL_PUBLICADA_Bruno_Leonardo_Marroig_de_Freitas_Ribeiro.pdf: 3116059 bytes, checksum: 0386ef826c193e02a06308f4b6f3aae3 (MD5)Made available in DSpace on 2021-01-06T20:38:55Z (GMT). No. of bitstreams: 1 DISSERTACAO_FINAL_PUBLICADA_Bruno_Leonardo_Marroig_de_Freitas_Ribeiro.pdf: 3116059 bytes, checksum: 0386ef826c193e02a06308f4b6f3aae3 (MD5) Previous issue date: 2011-06-30application/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Fisiopatologia e Ciências CirúrgicasUERJBRCentro Biomédico::Faculdade de Ciências MédicasProstateHyperplasiaProteoglycansImmunohistochemistrySyndecan-1DecorinBiglycanPróstataHiperplasiaProteoglicanosImunohistoquímicaSindecan-1DecorinBiglicanPróstata DoençasMatriz extracelularPróstata hipertrofiaProteoglicanasCNPQ::CIENCIAS DA SAUDE::MEDICINAAnálise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásicaImmunohistochemical analysis of the proteoglycans sindecan-1, decorin and biglican in the normal and hyperplastic prostateinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALDISSERTACAO_FINAL_PUBLICADA_Bruno_Leonardo_Marroig_de_Freitas_Ribeiro.pdfapplication/pdf3116059http://www.bdtd.uerj.br/bitstream/1/12363/1/DISSERTACAO_FINAL_PUBLICADA_Bruno_Leonardo_Marroig_de_Freitas_Ribeiro.pdf0386ef826c193e02a06308f4b6f3aae3MD511/123632024-02-26 16:45:29.626oai:www.bdtd.uerj.br:1/12363Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T19:45:29Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false
dc.title.por.fl_str_mv Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica
dc.title.alternative.eng.fl_str_mv Immunohistochemical analysis of the proteoglycans sindecan-1, decorin and biglican in the normal and hyperplastic prostate
title Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica
spellingShingle Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica
Ribeiro, Bruno Leonardo Marroig de Freitas
Prostate
Hyperplasia
Proteoglycans
Immunohistochemistry
Syndecan-1
Decorin
Biglycan
Próstata
Hiperplasia
Proteoglicanos
Imunohistoquímica
Sindecan-1
Decorin
Biglican
Próstata Doenças
Matriz extracelular
Próstata hipertrofia
Proteoglicanas
CNPQ::CIENCIAS DA SAUDE::MEDICINA
title_short Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica
title_full Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica
title_fullStr Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica
title_full_unstemmed Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica
title_sort Análise imunohistoquímica dos proteoglicanossindecan, decorin e biglican na próstata normal e hiperplásica
author Ribeiro, Bruno Leonardo Marroig de Freitas
author_facet Ribeiro, Bruno Leonardo Marroig de Freitas
author_role author
dc.contributor.advisor1.fl_str_mv Cardoso, Luiz Eduardo de Macedo
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3117933597142016
dc.contributor.advisor-co1.fl_str_mv Sampaio, Francisco José Barcellos
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/4426830285523015
dc.contributor.referee1.fl_str_mv Costa, Waldemar Silva
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/6986274610951714
dc.contributor.referee2.fl_str_mv Babinski, Marcio Antonio
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/7985386665524107
dc.contributor.referee3.fl_str_mv Fortes, Marco Antonio Quesada Ribeiro
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/9531672809534888
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/3575262832461071
dc.contributor.author.fl_str_mv Ribeiro, Bruno Leonardo Marroig de Freitas
contributor_str_mv Cardoso, Luiz Eduardo de Macedo
Sampaio, Francisco José Barcellos
Costa, Waldemar Silva
Babinski, Marcio Antonio
Fortes, Marco Antonio Quesada Ribeiro
dc.subject.eng.fl_str_mv Prostate
Hyperplasia
Proteoglycans
Immunohistochemistry
Syndecan-1
Decorin
Biglycan
topic Prostate
Hyperplasia
Proteoglycans
Immunohistochemistry
Syndecan-1
Decorin
Biglycan
Próstata
Hiperplasia
Proteoglicanos
Imunohistoquímica
Sindecan-1
Decorin
Biglican
Próstata Doenças
Matriz extracelular
Próstata hipertrofia
Proteoglicanas
CNPQ::CIENCIAS DA SAUDE::MEDICINA
dc.subject.por.fl_str_mv Próstata
Hiperplasia
Proteoglicanos
Imunohistoquímica
Sindecan-1
Decorin
Biglican
Próstata Doenças
Matriz extracelular
Próstata hipertrofia
Proteoglicanas
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::MEDICINA
description The cellular mechanisms involved in benign prostatic hyperplasia (BPH) are not well understood, and little is known about how proteoglycans are affected. Here we investigated the protein expression of stromal and cell surface proteoglycans in BPH. BPH samples were colected from open prostatectomy and transurethral resection of the prostate (TURP), while controls consisted of the transitional zone of normal prostates from young adults. We used primary antibodies against the proteoglycans syndecan-1, biglycan, and decorin. Immunolabeling was evaluated by determining the relative area of staining, or by using a score for staining intensity. The results showed that, in controls, syndecan-1 was mostly negative. In BPH, however, the labeling of this proteoglycan was intense and located mainly in acinar basal cells, but could extended into the secretory cells. As there were no differences in samples from open prostatectomy and TURP, these groups were combined into a BPH group. Syndecan-1 labeling area in the epithelium of BPH samples was nine times greater than that of controls (p<0,001), and there were no significant correlations between syndecan-1 labeling in BPH and prostate volume, PSA, or patient s age. Biglycan and decorin labeling were in the stroma exclusively, in control and BPH samples, and there were no significant differences in extent and intensity of the staining between these two groups. In conclusion, syndecan-1 immunolabeling in BPH is prominent and is located in the glandular epithelium exclusively, but intensity does not correlate with prostate size or PSA. Decorin and biglycan labeling, however, were unchanged in BPH.
publishDate 2011
dc.date.issued.fl_str_mv 2011-06-30
dc.date.available.fl_str_mv 2015-07-15
dc.date.accessioned.fl_str_mv 2021-01-06T20:38:55Z
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dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Fisiopatologia e Ciências Cirúrgicas
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro Biomédico::Faculdade de Ciências Médicas
publisher.none.fl_str_mv Universidade do Estado do Rio de Janeiro
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