Alterações no tecido esplênico de camundongos esquistossomóticos com desnutrição calórica e protéica induzida no período de lactação

• Autor(a) principal: Gomes, Adriana Cardoso
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14434
• topic: Schistosomiasis; Metabolic programming; Splenic tissue; Histopathology; Morphometry; Stereology; Esquistossomose; Programação metabólica; Tecido esplênico; Histopatologia; Morfometria. Estereologia; Esquistossomose mansonica; Schistosoma mansoni Parasitologia; Baço Doenças; Lactação Aspectos nutricionais; Deficiência de proteínas; Interações hospedeiro Parasita; CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA::HELMINTOLOGIA DE PARASITOS

Despite significant advances in the study of schistosomiasis, the relationship between schistosomiasis and malnutrition are still not completely understood. As lactation stage is a extreme important lifetime for the individual, metabolic abnormalities during pregnancy can directly affect fetal development suggesting a metabolic imprinting of this individual in adaptive response to environmental factors found in earlier periods of development. This study aimed to evaluate the spleen characteristics in the acute phase of mice schistosomiasis metabolically programmed by caloric restriction and protein restriction. The spleens of animals euthanized at the 9th week of infection were subjected to histological sections (5μm) and stained with hematoxylin-eosin. It was performed histopathological evaluation, morphometric and stereological analysis. Statistical analysis was performed using Graph Pad Instat. It was observed structural disorganization of the white pulp and the red pulp on programmed groups, regardless of the presence of infection. Infected animals showed hyperplasia and hypertrophy of the white pulp and a larger amount of pigments dispersed in the spleen tissue, as well as the presence of eosinophils within vascular structures. The white pulp of both infected groups of caloric restriction and protein restriction showed higher morphometric measurements when compared to uninfected groups. The stereological results showed that the infected caloric restriction group had lower volume density of red pulp, while there were no significant differences in the volume density of white pulp. Megakaryocytes were seen in higher quantities in the infected groups, with emphasis on the protein restriction group. These data suggest that imprinting by maternal undernutrition during lactation and schistosome infection causes disorganization on the splenic tissue.