Estudo estrutural e bioquímico da bexiga de ratos após tratamento com altas doses de corticosterona

• Autor(a) principal: Ribeiro, Gisele Silva
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/12345
• topic: bladder; corticosterone. morphometry; prepubertal; rats; bexiga; corticosterona; morfometria; pré-puberal; ratos; CNPQ::CIENCIAS DA SAUDE::MEDICINA::CIRURGIA::CIRURGIA UROLOGICA

The aim of this work was to analyze the structural and biochemical changes on the bladder wall resulting from the chronic treatment of prepubertal rats with high doses of corticosterone. This study included 26 male Wistar rats assigned to four groups: T30 was treated with corticosterone until 29 days of age and killed at day 30, while T65 group received the same treatment but was killed at day 65. Each group had its own control group (C30 and C65). For treated animals, daily intraperitoneal injections of corticosterone (2 mg/100g body weight) were administered between 7th and 29th day of life. Bladders were removed and collagen, smooth muscle, elastic fibers system, vascular density and epithelium were analyzed by morphometrical methods, immunofluorescence, and biochemistry.Vascular density in lamina propria was reduced by 40% (p<0.05) in group T65. Collagen organization was altered in T30 and T65, although total collagen concentration was unchanged. The T65 group had an increase in elastic system fibers. There was no difference in epithelial height and cell density between the groups. Concerning the smooth muscle fibers density we observed a 19% increase (p<0.05) in the T65 group. Our results show that prepubertal administration of corticosterone induces structural modifications in the bladder of rats