Utilização da terapia fotodinâmica associada ou não ao hipoclorito de sódio na instrumentação de canais radiculares contaminados com Enterococcus faecalis

• Autor(a) principal: Freitas, Lilian Ferreira
• Data de Publicação: 2009
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14029
• topic: Photodynamic therapy; Enterococcus faecalis; Antimicrobial action; Sodium hypochlorite; Terapia fotodinâmica; Enterococcus faecalis; Ação antimicrobiana; Hipoclorito de sódio; Fotoquimioterapia (Endodontia); Produtos de ação antimicrobiana; Canal radicular Tratamento; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::ENDODONTIA

This current paper aims to assess the action of low-intensity laser together with toluidine blue as a bactericidal agent during the instrumentation phase of radicular canals, comparing its associated or non-associated use with sodium hypochlorite 0.5%. To do so, 90 human canines were instrumented, autoclaved, and 88 were immersed into TSB, and were then inoculated with an E. faecalis suspension and brought into an incubator at 37 degrees Celsius for 72 hours in order to allow the formation of biofilm. The samples were randomly divided into 5 groups, 4 of which composed of 22 samples each plus 1 negative control. Group I: reinstrumented and irrigated with saline solution and subsequent application of photodynamic therapy (PDT); Group II: reinstrumented and irrigated with sodium hypochlorite 0.5% and subsequent PDT application; Group III: reinstrumented and irrigated with sodium hypochlorite 0.5%; Group IV: positive control (no previous treatment before material collection); Group V: negative control (2 samples) with no contamination. The collection of intracanal dentin was obtained by using a Gates Glidden drill #6. The antimicrobial activity was assessed by CFUs counting. The teeth were then immersed in a selected medium for Enterococcus, incubated at 37 degrees Celsius for 72 hours. They were then assessed for medium turbidity. All samples, with the exception of the negative control, showed positive. In order to make sure of the formation of biofilm and the confirmation of the CFUs counting, 9 samples were scavenged under the electron microscope. For result assessment, the Kruskal-Wallis test and the Tukey s multiple comparison test were utilized, presenting both tests a rate and significance of 5%. Based on the results obtained, we may licitly conclude that there was no significant statistical difference between the hypochlorite group and the hypochlorite group with laser. We may still conclude that PDT associated with saline solution instrumentation was not possible to significantly eliminate E. faecalis.