Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/12770 |
Resumo: | Studies show that overweight and obesity can affect male fertility. In addition, thyroid function also is associated with the alteration of testicular function, however, the physiological role of thyroid hormones in the regulation of male reproductive system is not yet clear. At 21 days of age, male rats were handled diet containing different concentrations of soy oil until the age of 30 and 60 days, the control group (C30 n=6 , C60 n=6), received a diet containing 7% and the high fat groups (HL30 n=6 , HL60n=6), received diet containing 19 % of this oil. At the end of each period, the animals were assessed by DXA (Absorptiometry X - ray Dual energy) and sacrificed by exsanguination. To assess changes in the structure of the testis and thyroid tissue morphology and stereological were performed. In plasma for determine the hormonal e biochemist profile were evaluated, triglycerides, total cholesterol, HDL cholesterol, VLDL, glucose and albumin by colorimetric assay and leptin, insulin, T4 , T3 , TSH and testosterone by radioimunoassay (RIA) . To demonstrate the expression of androgen receptor (AR) in testis, imunohistochemistry was performed with anti-AR. During the experimental period, body weight, food intake and body length were analyzed, and remained unchanged. Lean mass was lower at 30 days in the HL group, since the body fat total was higher in the same group, after 60 days, no difference was noted between the groups. In HL group at 30 days no difference was noted, and in same group at 60 days, the weight of the epididymis, liver and visceral fat remained higher. The biochemist profile in HL group at 30 days, there are no differences and at the 60 days in same group the level of glucose remained high. For hormonal dosages at 30 days in HL group, TSH and leptin were increased and T3 reduced, at 60 days in the same group, T3 and leptin were increased. Stereological and morphometric in the group Hl at 30 days, data show increases of number of seminiferous tubule and length density (Lv). At 60 days in the same group there are decreased of number of seminiferous tubule and diameter. The expression of androgen receptor (AR), in testis no differences are seems between the groups independent of the ages of consumption of diet. The fat diet promoted metabolic changes at 30 days and changes in the morphology of thyroid and testicular tissue at both ages, indicating an influence on reproductive function. |
id |
UERJ_61222e2e010082dfd48a48c44d2e5b3b |
---|---|
oai_identifier_str |
oai:www.bdtd.uerj.br:1/12770 |
network_acronym_str |
UERJ |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UERJ |
repository_id_str |
2903 |
spelling |
Saba, Celly Cristina Alves do Nascimentohttp://lattes.cnpq.br/0996290041727570Santos, Rachel Moreira Morais doshttp://lattes.cnpq.br/2736657601559318Figueiredo, Mariana Sartohttp://lattes.cnpq.br/6112802653312983Costa, Waldemar Silvahttp://lattes.cnpq.br/6986274610951714http://lattes.cnpq.br/6532224804585303Silva, Cynthia Carneiro Soares da2021-01-06T20:58:17Z2015-04-132014-02-12SILVA, Cynthia Carneiro Soares da. Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens. 2014. 84 f. Dissertação (Mestrado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2014.http://www.bdtd.uerj.br/handle/1/12770Studies show that overweight and obesity can affect male fertility. In addition, thyroid function also is associated with the alteration of testicular function, however, the physiological role of thyroid hormones in the regulation of male reproductive system is not yet clear. At 21 days of age, male rats were handled diet containing different concentrations of soy oil until the age of 30 and 60 days, the control group (C30 n=6 , C60 n=6), received a diet containing 7% and the high fat groups (HL30 n=6 , HL60n=6), received diet containing 19 % of this oil. At the end of each period, the animals were assessed by DXA (Absorptiometry X - ray Dual energy) and sacrificed by exsanguination. To assess changes in the structure of the testis and thyroid tissue morphology and stereological were performed. In plasma for determine the hormonal e biochemist profile were evaluated, triglycerides, total cholesterol, HDL cholesterol, VLDL, glucose and albumin by colorimetric assay and leptin, insulin, T4 , T3 , TSH and testosterone by radioimunoassay (RIA) . To demonstrate the expression of androgen receptor (AR) in testis, imunohistochemistry was performed with anti-AR. During the experimental period, body weight, food intake and body length were analyzed, and remained unchanged. Lean mass was lower at 30 days in the HL group, since the body fat total was higher in the same group, after 60 days, no difference was noted between the groups. In HL group at 30 days no difference was noted, and in same group at 60 days, the weight of the epididymis, liver and visceral fat remained higher. The biochemist profile in HL group at 30 days, there are no differences and at the 60 days in same group the level of glucose remained high. For hormonal dosages at 30 days in HL group, TSH and leptin were increased and T3 reduced, at 60 days in the same group, T3 and leptin were increased. Stereological and morphometric in the group Hl at 30 days, data show increases of number of seminiferous tubule and length density (Lv). At 60 days in the same group there are decreased of number of seminiferous tubule and diameter. The expression of androgen receptor (AR), in testis no differences are seems between the groups independent of the ages of consumption of diet. The fat diet promoted metabolic changes at 30 days and changes in the morphology of thyroid and testicular tissue at both ages, indicating an influence on reproductive function.Estudos demonstram que o sobrepeso e a obesidade podem afetar a fertilidade masculina. Além disso, a função tireóidea também esta associada à alteração da função testicular, entretanto, o papel fisiológico dos hormônios tireoideanos na regulação do sistema reprodutor masculino ainda não esta claro. Aos 21 dias de idade, ratos machos receberam uma dieta manipulada contendo diferentes concentrações de óleo de soja até a idade de 30 e 60 dias, os grupos controle (C30, n=6 e C60, n=6), receberam dieta contendo 7% e os grupos hiperlipídicos (HL30, n=6 e HL60, n=6), receberam dieta contendo 19% deste óleo. Ao final de cada período, os animais foram avaliados por DXA (Absorciometria de Raios-x em Duas Energias) e sacrificados por exsanguinação. Para avaliar alterações na estrutura dos tecidos testicular e tireóideo, foram realizados a morfologia e a estereologia. No plasma, para determinar os perfis bioquímico e hormonal, foram avaliados, triglicerídeos, colesterol total, HDL-colesterol, VLDL, glicose e albumina, por métodos colorimétricos e leptina, insulina, T4, T3, TSH e testosterona por radioimunoensaio (RIE). Para evidenciar a expressão de receptor androgênico (AR) em testículos, foi realizado imunomarcação, com anti-AR. Durante todo o período experimental, foram analisados a massa corporal, a ingestão alimentar e o comprimento corporal, os quais permaneceram inalterados. No grupo HL, a massa magra foi menor aos 30 dias, já a gordura corporal total foi maior no mesmo grupo, aos 60 dias nenhuma diferença foi notada entre os grupos. No grupo HL30 não houve diferença quanto à massa dos tecidos, já no grupo HL60, o peso do epidídimo, fígado e gordura visceral mantiveram-se aumentados. No grupo HL30 não houve diferença em relação ao perfil bioquímico, já no grupo HL60, os níveis de glicose, mantiveram-se altos. Quanto às dosagens hormonais no grupo HL30, TSH e leptina estiveram aumentados e T3 reduzido, e no grupo HL60, T3 e leptina estiveram aumentados. Os dados morfométricos e estereológicos de testículo no grupo HL30 mostram aumento no número de túbulos seminíferos e da densidade de comprimento (Lv), já no grupo HL60, há redução no número de túbulos seminíferos e no diâmetro do mesmo. Quanto à expressão de receptor androgênico nas células testiculares, não parece haver diferença entre os grupos independente da idade de consumo da dieta. A dieta hiperlipídica promoveu alterações metabólicas aos 30 dias e modificações na morfologia do tecido tireoidiano e testicular em ambas as idades, o que indica reflexos na função reprodutora.Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:58:17Z No. of bitstreams: 1 Cynthia Carneiro Soares da Silva Dissertacao completa.pdf: 3121155 bytes, checksum: dfb0bb4b8241a93c08367a2ebce032b5 (MD5)Made available in DSpace on 2021-01-06T20:58:17Z (GMT). No. of bitstreams: 1 Cynthia Carneiro Soares da Silva Dissertacao completa.pdf: 3121155 bytes, checksum: dfb0bb4b8241a93c08367a2ebce032b5 (MD5) Previous issue date: 2014-02-12Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Fisiopatologia Clínica e ExperimentalUERJBRCentro Biomédico::Faculdade de Ciências MédicasHigh-fat dietMale reproductionStereologyDieta hiperlipídicaReprodução masculinaEstereologiaGorduras na dietaEstereologiaReprodução humana HomensTestículoCNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINADieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovensHigh fat diet to promise the morphology and some functional parameters of testis and thyroid of Young male ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALCynthia Carneiro Soares da Silva Dissertacao completa.pdfapplication/pdf3121155http://www.bdtd.uerj.br/bitstream/1/12770/1/Cynthia+Carneiro+Soares+da+Silva+Dissertacao+completa.pdfdfb0bb4b8241a93c08367a2ebce032b5MD511/127702024-02-26 16:36:39.97oai:www.bdtd.uerj.br:1/12770Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T19:36:39Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens |
dc.title.alternative.eng.fl_str_mv |
High fat diet to promise the morphology and some functional parameters of testis and thyroid of Young male rats |
title |
Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens |
spellingShingle |
Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens Silva, Cynthia Carneiro Soares da High-fat diet Male reproduction Stereology Dieta hiperlipídica Reprodução masculina Estereologia Gorduras na dieta Estereologia Reprodução humana Homens Testículo CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINA |
title_short |
Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens |
title_full |
Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens |
title_fullStr |
Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens |
title_full_unstemmed |
Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens |
title_sort |
Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens |
author |
Silva, Cynthia Carneiro Soares da |
author_facet |
Silva, Cynthia Carneiro Soares da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Saba, Celly Cristina Alves do Nascimento |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0996290041727570 |
dc.contributor.referee1.fl_str_mv |
Santos, Rachel Moreira Morais dos |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2736657601559318 |
dc.contributor.referee2.fl_str_mv |
Figueiredo, Mariana Sarto |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6112802653312983 |
dc.contributor.referee3.fl_str_mv |
Costa, Waldemar Silva |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/6986274610951714 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6532224804585303 |
dc.contributor.author.fl_str_mv |
Silva, Cynthia Carneiro Soares da |
contributor_str_mv |
Saba, Celly Cristina Alves do Nascimento Santos, Rachel Moreira Morais dos Figueiredo, Mariana Sarto Costa, Waldemar Silva |
dc.subject.eng.fl_str_mv |
High-fat diet Male reproduction Stereology |
topic |
High-fat diet Male reproduction Stereology Dieta hiperlipídica Reprodução masculina Estereologia Gorduras na dieta Estereologia Reprodução humana Homens Testículo CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINA |
dc.subject.por.fl_str_mv |
Dieta hiperlipídica Reprodução masculina Estereologia Gorduras na dieta Estereologia Reprodução humana Homens Testículo |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINA |
description |
Studies show that overweight and obesity can affect male fertility. In addition, thyroid function also is associated with the alteration of testicular function, however, the physiological role of thyroid hormones in the regulation of male reproductive system is not yet clear. At 21 days of age, male rats were handled diet containing different concentrations of soy oil until the age of 30 and 60 days, the control group (C30 n=6 , C60 n=6), received a diet containing 7% and the high fat groups (HL30 n=6 , HL60n=6), received diet containing 19 % of this oil. At the end of each period, the animals were assessed by DXA (Absorptiometry X - ray Dual energy) and sacrificed by exsanguination. To assess changes in the structure of the testis and thyroid tissue morphology and stereological were performed. In plasma for determine the hormonal e biochemist profile were evaluated, triglycerides, total cholesterol, HDL cholesterol, VLDL, glucose and albumin by colorimetric assay and leptin, insulin, T4 , T3 , TSH and testosterone by radioimunoassay (RIA) . To demonstrate the expression of androgen receptor (AR) in testis, imunohistochemistry was performed with anti-AR. During the experimental period, body weight, food intake and body length were analyzed, and remained unchanged. Lean mass was lower at 30 days in the HL group, since the body fat total was higher in the same group, after 60 days, no difference was noted between the groups. In HL group at 30 days no difference was noted, and in same group at 60 days, the weight of the epididymis, liver and visceral fat remained higher. The biochemist profile in HL group at 30 days, there are no differences and at the 60 days in same group the level of glucose remained high. For hormonal dosages at 30 days in HL group, TSH and leptin were increased and T3 reduced, at 60 days in the same group, T3 and leptin were increased. Stereological and morphometric in the group Hl at 30 days, data show increases of number of seminiferous tubule and length density (Lv). At 60 days in the same group there are decreased of number of seminiferous tubule and diameter. The expression of androgen receptor (AR), in testis no differences are seems between the groups independent of the ages of consumption of diet. The fat diet promoted metabolic changes at 30 days and changes in the morphology of thyroid and testicular tissue at both ages, indicating an influence on reproductive function. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-02-12 |
dc.date.available.fl_str_mv |
2015-04-13 |
dc.date.accessioned.fl_str_mv |
2021-01-06T20:58:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Cynthia Carneiro Soares da. Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens. 2014. 84 f. Dissertação (Mestrado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2014. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/12770 |
identifier_str_mv |
SILVA, Cynthia Carneiro Soares da. Dieta hiperlipídica compromete a morfologia e alguns parâmetros funcionais de testículo e tireóide de ratos jovens. 2014. 84 f. Dissertação (Mestrado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2014. |
url |
http://www.bdtd.uerj.br/handle/1/12770 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Estado do Rio de Janeiro |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Fisiopatologia Clínica e Experimental |
dc.publisher.initials.fl_str_mv |
UERJ |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Centro Biomédico::Faculdade de Ciências Médicas |
publisher.none.fl_str_mv |
Universidade do Estado do Rio de Janeiro |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UERJ instname:Universidade do Estado do Rio de Janeiro (UERJ) instacron:UERJ |
instname_str |
Universidade do Estado do Rio de Janeiro (UERJ) |
instacron_str |
UERJ |
institution |
UERJ |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UERJ |
collection |
Biblioteca Digital de Teses e Dissertações da UERJ |
bitstream.url.fl_str_mv |
http://www.bdtd.uerj.br/bitstream/1/12770/1/Cynthia+Carneiro+Soares+da+Silva+Dissertacao+completa.pdf |
bitstream.checksum.fl_str_mv |
dfb0bb4b8241a93c08367a2ebce032b5 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ) |
repository.mail.fl_str_mv |
bdtd.suporte@uerj.br |
_version_ |
1792352316990423040 |