Interação in vitro de cepas de Aeromonas caviae com células T-84 e Hep-2 quanto à capacidade de invadir, persistir e induzir morte celular

• Autor(a) principal: Alves, Maria Angélica de Mattos
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14382
• topic: Aeromonas caviae; Invasion; Intracellular persistence; Cell death; Aeromonas caviae; Invasão; Persistência intracelular; Morte celular; Aeromonas; Virulência (Microbiologia); Morte celular; Fatores de virulência; CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA

Initially considered fish pathogens, today Aeromonas are considered emerging human pathogens. The genus has wide environmental distribution and is responsible for intestinal and extraintestinal infections ranging from self-limited to severe cases. The disease caused by this pathogen is favored by many virulence factors, including exotoxins, hemolysin, siderophores, flagella, ability to adhere to and invade cells and formation of biofilms. This study analyzes certain pathogenic mechanisms mediated by A. caviae evaluating the participation of micro-organism in cell colonization processes. Qualitative and quantitative in vitro assays were used to test the cytotoxicity, adhesion, invasion, intracellular persistence and potential to induce cell death in HEp-2 cell lines and / or T-84. The five strains of A. caviae studied previously isolated fish showed pattern of aggregative adherence to T-84 but were not cytotoxic to this cell line. The five strains were able to invade HEp-2 cells and T-84 at 6 hours of incubation. These strains were subjected to the quantitative assay of intracellular persistence and survived for up to 48h in HEp-2 cells and 72 hours for T-84 cells. All strains were able to induce typical morphological features of apoptosis and necrosis in both cell lines used, such as fragmentation and chromatin condensation and nuclear increase, although at different times according to the cell line. Finally, our results contributed to reinforcing the pathogenic potential of strains of Aeromonas caviae foodborne.