Caracterização molecular e estrutural de tecido ósseo exposto à água de rio

• Autor(a) principal: Santos, Fernanda Carvalho
• Data de Publicação: 2018
• Outros Autores: [e-mail]
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/18171
• topic: DNA; Forensic; Bones; River; DNA; Forense; Ossos; Rio; CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR

Different subjects of Forensic Science are usually used in human remains identification and Forensic Genetic is the most advanced. This use is related for the force of DNA in the courts, been necessary more studies focus in skeletal cases. Skeletal Remains are common found in forensic scenarios, wars and wide catastrophes, involving military and civil persons, in aquatic environments or near to. In all cases DNA it’s the most important tool for identify them. However, published articles shows difficulty in generate genetic profile from this individual and, then, identify yours bodies. Here the objective is identified changes in bones samples immersed, in structure and genetics levels, for increasing the knowledge in similar cases. Was used bones fragments from Diagnostics Laboratory by DNA (UERJ) exposed to river water for 30. 60 and 90 days. For structure analysis was used SEM-EDS and Raman Spectroscopy Quantitative PCR and Agarose Gel was performed for genetic knowledge. Was observed morphologic modifications in both cortical and trabecular bones. Change in ionic composition was observed more strongly in external surface and trabecular bone, with, less percentage in fundamentals components and in increasing in elements like Manganese, Iron, Aluminum and Silicon. DNA quantity have no changes in the experiment time. Only positive ions were founded in our analyses. Quantification results shows no significant differences in DNA quantity between control and exposed samples. Agarose Gel deduce no changes in quality of mitochondrial DNA in our experiment time. This contributes for hypotheses that ions and DNA interaction occur and, because of that, changes in chemical structural are triggered. This interaction can interfere in the annealing process in PCR, when less primers annealing to the DNA tape and make the amplification series. For future, new analysis focus in DNA structure will be necessary for confirmed the hypothesis.