Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/7773 |
Resumo: | Diets rich in saturated fat have a deleterious effect on glucose metabolism, adipokine release and inflammation. However, other lipid sources may modulate these parameters differently. Thus, the aim of the present work was to investigate the effect of high-fat diets from different sources on carbohydrate and lipid metabolism, adipose tissue and liver. Male C57BL/6 mice were allocated into 5 groups (n=10/group): standard chow fed mice (SC, 10% lipid, control group), and mice fed with high-fat diets (HF, 60% lipids) as follow: lard (HF-L), olive oil (HF-O), sunflower oil (HF-S), and canola oil (HF-C). Diets were offered for 10 weeks. Food intake was recorded daily and body mass weekly. One week before euthanasia, fasting glucose and intraperitoneal insulin tolerance test (IPITT) were assessed. At euthanasia, blood was collected, and the liver and adipose tissue were weighed. Insulin, leptin, adiponectin, resistin, tumoral necrosis factor alpha (TNFα), interlekin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and plasminogen activator inhibitor-1 (PAI-1) were measured by an Elisa assay. The HOMA-IR index was calculated based on fasting glucose and insulin. Insulin, resistin, leptin and HOMA-IR were higher in HF-L and HF-O groups compared with the remainder groups (P < 0.0001). IL-6 was higher in the HF-L group compared with the other groups (P < 0.0005), whereas adiponectin levels were the lowest (P < 0.0001). The amount of both subcutaneous and visceral fat depots was the highest in the HF-L group, and they also had increased adipocyte diameter. However, visceral to subcutaneous fat ratio was higher in HF-L and HF-O groups compared with the remainder groups. Additionally, HF-L and HF-O groups showed increased hepatic triglyceride content and steatosis. Our data allow us to conclude that the type of lipid instead of its amount is important for determining adipose tissue distribution, carbohydrate metabolism, hepatic triglyceride accumulation and steatosis in mice fed a high-fat diet. |
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Lacerda, Márcia Barbosa Águila Mandarim dehttp://lattes.cnpq.br/0119459843172158Ribeiro, Antônio Cláudio Mendeshttp://lattes.cnpq.br/4764377589833985Mandarim-de-Lacerda, Carlos Albertohttp://lattes.cnpq.br/2960155071929174Brunini, Tatiana Marlowe Cunhahttp://lattes.cnpq.br/1794383786839231Vianna, Lúcia Marques Alveshttp://lattes.cnpq.br/8587746890753274Dôres, Sílvia Maria Custódio dashttp://lattes.cnpq.br/0407406842124499http://lattes.cnpq.br/4257917767685033Pereira, Mariana Catta-preta Caiado2021-01-05T18:07:55Z2014-05-222011-02-21PEREIRA, Mariana Catta-preta Caiado. Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos. 2011. 99 f. Tese (Doutorado em Biologia Humana e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2011.http://www.bdtd.uerj.br/handle/1/7773Diets rich in saturated fat have a deleterious effect on glucose metabolism, adipokine release and inflammation. However, other lipid sources may modulate these parameters differently. Thus, the aim of the present work was to investigate the effect of high-fat diets from different sources on carbohydrate and lipid metabolism, adipose tissue and liver. Male C57BL/6 mice were allocated into 5 groups (n=10/group): standard chow fed mice (SC, 10% lipid, control group), and mice fed with high-fat diets (HF, 60% lipids) as follow: lard (HF-L), olive oil (HF-O), sunflower oil (HF-S), and canola oil (HF-C). Diets were offered for 10 weeks. Food intake was recorded daily and body mass weekly. One week before euthanasia, fasting glucose and intraperitoneal insulin tolerance test (IPITT) were assessed. At euthanasia, blood was collected, and the liver and adipose tissue were weighed. Insulin, leptin, adiponectin, resistin, tumoral necrosis factor alpha (TNFα), interlekin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and plasminogen activator inhibitor-1 (PAI-1) were measured by an Elisa assay. The HOMA-IR index was calculated based on fasting glucose and insulin. Insulin, resistin, leptin and HOMA-IR were higher in HF-L and HF-O groups compared with the remainder groups (P < 0.0001). IL-6 was higher in the HF-L group compared with the other groups (P < 0.0005), whereas adiponectin levels were the lowest (P < 0.0001). The amount of both subcutaneous and visceral fat depots was the highest in the HF-L group, and they also had increased adipocyte diameter. However, visceral to subcutaneous fat ratio was higher in HF-L and HF-O groups compared with the remainder groups. Additionally, HF-L and HF-O groups showed increased hepatic triglyceride content and steatosis. Our data allow us to conclude that the type of lipid instead of its amount is important for determining adipose tissue distribution, carbohydrate metabolism, hepatic triglyceride accumulation and steatosis in mice fed a high-fat diet.As dietas ricas em lipídios saturados provocam efeitos deletérios no metabolismo de glicose, secreção de adipocinas e inflamação, entretanto, outros tipos de lipídios podem modular de forma diferenciada tais efeitos. Assim, o objetivo deste trabalho foi investigar o efeito de diferentes dietas hiperlipídicas no metabolismo de carboidratos, lipídios, no tecido adiposo e no fígado. Camundongos machos C57BL/6 foram divididos em 5 grupos (n=10/grupo): animais que receberam dieta controle (standart chow, SC, 10% de lipídios, grupo controle) e animais que receberam diferentes dietas hiperlipídicas (High-fat, HF, 60% de lipídios): à base de banha de porco (lard, grupo HF-L), à base de óleo de oliva (olive oil, grupo HF-O), à base de óleo de girassol (sunflower oil, grupo HF-S) e à base de óleo de canola (canola oil, grupo HF-Ca).Os animais foram alimentados com as dietas experimentas por 10 semanas. Diariamente a ingestão alimentar era verificada e semanalmente a massa corporal foi aferida. A glicose de jejum e o teste intraperitoneal de tolerância a insulina (TITI) foram realizados uma semana antes da eutanásia. No dia da eutanásia o sangue foi coletado, o tecido adiposo e o fígado dissecados e pesados. A insulina, leptina, adiponectina, resistina, fator de necrose tumoral alfa (TNFα), interleucina-6 (IL-6), proteína quimiotática de monócitos-1 (MCP-1) e inibidor do ativador de plasminogênio-1 (PAI-1) foram dosadas por ELISA. Com os dados de insulina e glicose foi calculado o índice HOMA-IR. Os animais dos grupos HF-L e HF-O apresentaram os maiores valores de insulina, resistina, leptina e HOMA-IR em comparação aos outros grupos (P < 0,0001). No grupo HF-L, os níveis de IL-6 foram maiores quando comparados com os demais grupos (P < 0,0005), enquanto os valores de adiponectina foram os menores (P < 0,0001). A quantidade de gordura subcutânea e visceral foi maior no grupo HF-L e este grupo apresentou também um aumento no diâmetro dos adipócitos. Entretanto a relação:visceral:subcutânea foi maior nos grupos HF-L e HF-O quando comparado com os demais grupos. Além disso, houve aumento de triglicérides hepáticos e de esteatose hepática nos animais dos grupos HF-L e HF-O. Nossos achados nos permitem concluir que animais que são alimentados com dietas hiperlipídica, a distribuição do tecido adiposo, o metabolismo de carboidratos, acumulo de triglicérides hepáticos e esteatose hepática são mais influenciados pelo tipo de lipídios do que pela quantidade.Submitted by Boris Flegr (boris@uerj.br) on 2021-01-05T18:07:55Z No. of bitstreams: 1 Tese Mariana Catta-Preta.pdf: 2816546 bytes, checksum: 4c4c614441da767e926f46fc719d9aff (MD5)Made available in DSpace on 2021-01-05T18:07:55Z (GMT). No. of bitstreams: 1 Tese Mariana Catta-Preta.pdf: 2816546 bytes, checksum: 4c4c614441da767e926f46fc719d9aff (MD5) Previous issue date: 2011-02-21application/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Biologia Humana e ExperimentalUERJBRCentro Biomédico::Instituto de Biologia Roberto Alcantara GomesHigh-fat dietCanola oilOlive oilSunflower oilLardDieta hiperlipídicaÓleo de canolaÓleo de olivaÓleo de girassolBanha de porcoLipídios MetabolismoGorduras na dieta MetabolismoGorduras na dieta Administração e dosagemÓleos vegetaisFígado MetabolismoCNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIADietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongosFat diets alter inflammatory cytokines, adipokines, adipose tissue and liver in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALTese Mariana Catta-Preta.pdfapplication/pdf2816546http://www.bdtd.uerj.br/bitstream/1/7773/1/Tese+Mariana+Catta-Preta.pdf4c4c614441da767e926f46fc719d9affMD511/77732024-02-26 15:24:03.177oai:www.bdtd.uerj.br:1/7773Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T18:24:03Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos |
dc.title.alternative.eng.fl_str_mv |
Fat diets alter inflammatory cytokines, adipokines, adipose tissue and liver in mice |
title |
Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos |
spellingShingle |
Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos Pereira, Mariana Catta-preta Caiado High-fat diet Canola oil Olive oil Sunflower oil Lard Dieta hiperlipídica Óleo de canola Óleo de oliva Óleo de girassol Banha de porco Lipídios Metabolismo Gorduras na dieta Metabolismo Gorduras na dieta Administração e dosagem Óleos vegetais Fígado Metabolismo CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA |
title_short |
Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos |
title_full |
Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos |
title_fullStr |
Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos |
title_full_unstemmed |
Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos |
title_sort |
Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos |
author |
Pereira, Mariana Catta-preta Caiado |
author_facet |
Pereira, Mariana Catta-preta Caiado |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lacerda, Márcia Barbosa Águila Mandarim de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0119459843172158 |
dc.contributor.advisor-co1.fl_str_mv |
Ribeiro, Antônio Cláudio Mendes |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/4764377589833985 |
dc.contributor.referee1.fl_str_mv |
Mandarim-de-Lacerda, Carlos Alberto |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2960155071929174 |
dc.contributor.referee2.fl_str_mv |
Brunini, Tatiana Marlowe Cunha |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1794383786839231 |
dc.contributor.referee3.fl_str_mv |
Vianna, Lúcia Marques Alves |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/8587746890753274 |
dc.contributor.referee4.fl_str_mv |
Dôres, Sílvia Maria Custódio das |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/0407406842124499 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4257917767685033 |
dc.contributor.author.fl_str_mv |
Pereira, Mariana Catta-preta Caiado |
contributor_str_mv |
Lacerda, Márcia Barbosa Águila Mandarim de Ribeiro, Antônio Cláudio Mendes Mandarim-de-Lacerda, Carlos Alberto Brunini, Tatiana Marlowe Cunha Vianna, Lúcia Marques Alves Dôres, Sílvia Maria Custódio das |
dc.subject.eng.fl_str_mv |
High-fat diet Canola oil Olive oil Sunflower oil Lard |
topic |
High-fat diet Canola oil Olive oil Sunflower oil Lard Dieta hiperlipídica Óleo de canola Óleo de oliva Óleo de girassol Banha de porco Lipídios Metabolismo Gorduras na dieta Metabolismo Gorduras na dieta Administração e dosagem Óleos vegetais Fígado Metabolismo CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA |
dc.subject.por.fl_str_mv |
Dieta hiperlipídica Óleo de canola Óleo de oliva Óleo de girassol Banha de porco Lipídios Metabolismo Gorduras na dieta Metabolismo Gorduras na dieta Administração e dosagem Óleos vegetais Fígado Metabolismo |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::MORFOLOGIA |
description |
Diets rich in saturated fat have a deleterious effect on glucose metabolism, adipokine release and inflammation. However, other lipid sources may modulate these parameters differently. Thus, the aim of the present work was to investigate the effect of high-fat diets from different sources on carbohydrate and lipid metabolism, adipose tissue and liver. Male C57BL/6 mice were allocated into 5 groups (n=10/group): standard chow fed mice (SC, 10% lipid, control group), and mice fed with high-fat diets (HF, 60% lipids) as follow: lard (HF-L), olive oil (HF-O), sunflower oil (HF-S), and canola oil (HF-C). Diets were offered for 10 weeks. Food intake was recorded daily and body mass weekly. One week before euthanasia, fasting glucose and intraperitoneal insulin tolerance test (IPITT) were assessed. At euthanasia, blood was collected, and the liver and adipose tissue were weighed. Insulin, leptin, adiponectin, resistin, tumoral necrosis factor alpha (TNFα), interlekin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and plasminogen activator inhibitor-1 (PAI-1) were measured by an Elisa assay. The HOMA-IR index was calculated based on fasting glucose and insulin. Insulin, resistin, leptin and HOMA-IR were higher in HF-L and HF-O groups compared with the remainder groups (P < 0.0001). IL-6 was higher in the HF-L group compared with the other groups (P < 0.0005), whereas adiponectin levels were the lowest (P < 0.0001). The amount of both subcutaneous and visceral fat depots was the highest in the HF-L group, and they also had increased adipocyte diameter. However, visceral to subcutaneous fat ratio was higher in HF-L and HF-O groups compared with the remainder groups. Additionally, HF-L and HF-O groups showed increased hepatic triglyceride content and steatosis. Our data allow us to conclude that the type of lipid instead of its amount is important for determining adipose tissue distribution, carbohydrate metabolism, hepatic triglyceride accumulation and steatosis in mice fed a high-fat diet. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-21 |
dc.date.available.fl_str_mv |
2014-05-22 |
dc.date.accessioned.fl_str_mv |
2021-01-05T18:07:55Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, Mariana Catta-preta Caiado. Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos. 2011. 99 f. Tese (Doutorado em Biologia Humana e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2011. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/7773 |
identifier_str_mv |
PEREIRA, Mariana Catta-preta Caiado. Dietas hiperlipídicas alteram citocinas inflamatórias, adipocinas, tecido adiposo e fígado em camundongos. 2011. 99 f. Tese (Doutorado em Biologia Humana e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2011. |
url |
http://www.bdtd.uerj.br/handle/1/7773 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade do Estado do Rio de Janeiro |
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Programa de Pós-Graduação em Biologia Humana e Experimental |
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UERJ |
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BR |
dc.publisher.department.fl_str_mv |
Centro Biomédico::Instituto de Biologia Roberto Alcantara Gomes |
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Universidade do Estado do Rio de Janeiro |
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reponame:Biblioteca Digital de Teses e Dissertações da UERJ instname:Universidade do Estado do Rio de Janeiro (UERJ) instacron:UERJ |
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Biblioteca Digital de Teses e Dissertações da UERJ |
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Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ) |
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