A influência do extrato de Ginkgo biloba 761 na resposta inflamatória renal em ratos alimentados com dieta hiperlipidica a base de amendoim in natura

• Autor(a) principal: Camara, Monica Dalmacio Silveira Campos
• Data de Publicação: 2016
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/12671
• topic: Kidney; Ginkgo biloba; Inflammation; Peanuts; Lipids; Rins; Ginkgo biloba; Inflamação; Amendoim; Lipídeos; Rins; Ginkgo biloba; Gorduras na dieta; Amendoim; CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::NEFROLOGIA

The high fat diet (HFD) is composed for 35% or more in lipids daily and promotes inflammation in many organs like kidney. Ginkgo biloba extract 761 (GBE) is used as an anti-inflammatory herbal medicine and for treatment the cerebral and peripheral circulatory disorders. Wistar rats (N=20) were divided in 4 groups: G1 received peanuts (high fat diet/HFD) and saline (water and sodium chloride solution 0.9%), G2 peanuts (HFD) and 2mg/kg of GBE, whereas G3 received commercial diet and saline and G4 received CD and GBE. All groups at the protocol were observed for 80 days (day 70-80, started the treatment with GBE or saline). The food intake was measured daily. Histological assessments were performed and serum was collected 3 times for ELISA the Immunoglobulin G (IgG1 and IgG2a), Thromboxane B2 (TBX2) and cholesterol and triglycerides. Statistical test used ANOVA with Fisher or Tukey s post test. No significant difference (p>0.05) was observed in food intake, weight or cholesterol total and triglycerides of the animals. Animals feed with HFD and treated with GBE obtained a reduction in inflammatory markers: NFKβ (p<0.05) and IL6, TGFβ. Blue toluidine (Mast cells) did not differ. PCNA (Proliferating cell nuclear antigen) had a significant difference (P<0.05) for the animals feed with HFD and treated with GBE. The ELISA IgG2a shows a significant difference (P <0.05) at the beginning the HFD and long exposition to a HFD, the TBX2 (thromboxane B2) shows differences (p>0.05) from the animals feeding with HFD or commercial diet and after the introduction of GBE. The high fat diet promotes inflammation of the kidneys in the health rats and an introduction of GBE (therapeutic dose) in rats feed with a high fat diet may promote a protection in the physiology and inflammatory markers of the kidney.