Eficácia in vitro dos sistemas ProTaper Next®, ProTaper Universal® e WaveOne® na redução da população de Enterococcus faecalis de canais radiculares

• Autor(a) principal: Brito, Patrícia Reis Rezende de
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14048
• topic: Endodontic treatment; Sodium hypochlorite; Enterococcus faecalis; Protaper Next; Tratamento endodôntico; Hipoclorito de sódio; Enterococcus faecalis; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::ENDODONTIA

This study constitutes an attempt to evaluate the effectiveness of ProTaper Next® (PTN), in reducing the intra canal Enterococcus faecalis count compared with ProTaper Universal® (PTU) and WaveOne® (WO) rotary instrument. A hundred teeth single-rooted human mandibular premolars were selected and ninety-five were contaminated with E. faecalis suspension at 37o C for 4 weeks and then randomly divided into 6 groups (n=15) according to the instrumentation system and the irrigation applied: PTN + 2.5% NaOCl; PTU + 2.5% NaOCl; WO + 2.5% NaOCl; PTN + saline solution; PTU + saline solution and WO + saline solution. Positive and negative control groups were created with 5 specimens each. Canals were prepared up to an apical size 40 at the working length (WL) and the irrigation volume was standardized for all experimental groups. Samples taken before (S1) and after (S2) the chemo mechanical procedures were sampled and plated onto Mitis-salivarius agar plates and incubated at 37C for 48 hours and the colony forming units (CFUs) were counted. The paired t-test was used for intragroup analysis, and one-way ANOVA for intergroup analysis. When significant differences were found among the groups, the Tukey test was used. The significance levels were set at 5% (P<0.05). After root canal instrumentation (S2), there was a highly significant reduction in bacterial load in all groups (P<0.01). WO/saline solution group showed a lower level of bacterial reduction when compared with the other tested groups (P<0.01). Groups irrigated with NaOCl showed a higher level of bacterial reduction when compared with saline solution irrigation (P<0.01), with no statistical differences among systems used (P>0.05). Can be concluded that PTN system was as effective as PTU and WO for removal of bacteria during endodontic treatment.