Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/14192 |
Resumo: | Denture soft lining materials are widely used after dental surgeries, tissue conditioning and stabilization of prostheses, until the complete tissue healing. It is important that this material not became easily colonized by oral biofilm and if possible, avoid contamination of the surgical site. The aim of this study was to evaluate the effect of chlorhexidine incorporation into resin-based soft denture lining material, considering the drug release analysis, cytotoxicity and C. albicans biofilm inhibition. Specimens were done using Trusoft® and Coe-soft®, incorporating 0%, 0.5%, 1.0% and 2.0% of chlorhexidine, totaling eight groups. The chlorhexidine release was evaluated through the measurement of change in optical density of the storage solution, which the specimens were immersed, by UV spectrometry, after every 48 hours for 40 days. The cellular cytotoxicity was evaluated in fibroblasts (strain L929), which were 24 hours in contact with culture medium, in which the specimens were previously immersed, the technique of neutral red dye uptake was used after 24, 48 and 72 hours and weekly until 28th day. Finally, the antifungal activity against C. albicans (ATCC 10231) was evaluated by two different ways: (1) agar diffusion test, in which the specimens were placed over the top of BHI agar plates previously inoculated with C. albicans, and the measurement of inhibition zone was done after 48h of incubation at 37ºC, (2) C. albicans biofilm inhibition over the specimens surface, which was mensured after every each 48 hours of biofilm and specimens co-incubation, by methyl tetrazolium (MTT), in UV/vis spectrophotometer, during 22 days. Data were analyzed with the SigmaStat software (version 3.1, USA). Statistical differences were determined by analysis of variance ANOVA and all procedures for multiple paired comparisons were made using the Holm-Sidak method, with overall significance level of 0.05. The chlorhexidine added to both resins, Trusoft® and Coe-soft®, can be released to the storage medium, with a dose-related effect, however the resins presented different release kinetics, since the Trusof released up to 71% of the total amount of the chlorhexidine released within the first 48 hours and Coe-soft release only up to 44%, considering the citotoxic effect, both chlorhexidine incorporated resins showed some extra cytotoxic effect, if compared to resins without chlorhexidine, but for Coe-soft no statistical difference of values was founded, only for Trusoft (p<0.001); considering the C. albicans inhibition, the agar diffusion test values were dose-related for both resins, however with bigger inhibition zones for Trusoft (p<0.001), and without any inhibition for the resins without clorexidine. For the C. albicans biofilm inhibition test, performed only with Coe-soft resin, it was verified a complete inhibition at 8, 12 and 16 days for the incorporation of 0.5%, 1.0% and 2.0% of clorexidine respectively, a statistically significant decrease (p<0.001) if compared to the resin without incorporation of chlorhexidine, which showed no inhibitory effect over the biofilm formation. |
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Telles, Daniel de Moraeshttp://lattes.cnpq.br/6024021400472077Portela, Maristela Barbosahttp://lattes.cnpq.br/7374992387363131Figueredo, Carlos Marcelo da Silvahttp://lattes.cnpq.br/0411336093247858Hirata Júnior, Raphaelhttp://lattes.cnpq.br/4484092525465200Cury, Altair Antoninha Del Belhttp://lattes.cnpq.br/0850165284804366http://lattes.cnpq.br/3167449614721645Bertolini, Martinna de Mendonça e2021-01-07T15:06:05Z2015-05-052011-12-07BERTOLINI, Martinna de Mendonça e. Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina. 2011. 54 f. Dissertação (Mestrado em Dentística; Endodontia; Odontopediatria; Ortodontia; Periodontia;) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2011.http://www.bdtd.uerj.br/handle/1/14192Denture soft lining materials are widely used after dental surgeries, tissue conditioning and stabilization of prostheses, until the complete tissue healing. It is important that this material not became easily colonized by oral biofilm and if possible, avoid contamination of the surgical site. The aim of this study was to evaluate the effect of chlorhexidine incorporation into resin-based soft denture lining material, considering the drug release analysis, cytotoxicity and C. albicans biofilm inhibition. Specimens were done using Trusoft® and Coe-soft®, incorporating 0%, 0.5%, 1.0% and 2.0% of chlorhexidine, totaling eight groups. The chlorhexidine release was evaluated through the measurement of change in optical density of the storage solution, which the specimens were immersed, by UV spectrometry, after every 48 hours for 40 days. The cellular cytotoxicity was evaluated in fibroblasts (strain L929), which were 24 hours in contact with culture medium, in which the specimens were previously immersed, the technique of neutral red dye uptake was used after 24, 48 and 72 hours and weekly until 28th day. Finally, the antifungal activity against C. albicans (ATCC 10231) was evaluated by two different ways: (1) agar diffusion test, in which the specimens were placed over the top of BHI agar plates previously inoculated with C. albicans, and the measurement of inhibition zone was done after 48h of incubation at 37ºC, (2) C. albicans biofilm inhibition over the specimens surface, which was mensured after every each 48 hours of biofilm and specimens co-incubation, by methyl tetrazolium (MTT), in UV/vis spectrophotometer, during 22 days. Data were analyzed with the SigmaStat software (version 3.1, USA). Statistical differences were determined by analysis of variance ANOVA and all procedures for multiple paired comparisons were made using the Holm-Sidak method, with overall significance level of 0.05. The chlorhexidine added to both resins, Trusoft® and Coe-soft®, can be released to the storage medium, with a dose-related effect, however the resins presented different release kinetics, since the Trusof released up to 71% of the total amount of the chlorhexidine released within the first 48 hours and Coe-soft release only up to 44%, considering the citotoxic effect, both chlorhexidine incorporated resins showed some extra cytotoxic effect, if compared to resins without chlorhexidine, but for Coe-soft no statistical difference of values was founded, only for Trusoft (p<0.001); considering the C. albicans inhibition, the agar diffusion test values were dose-related for both resins, however with bigger inhibition zones for Trusoft (p<0.001), and without any inhibition for the resins without clorexidine. For the C. albicans biofilm inhibition test, performed only with Coe-soft resin, it was verified a complete inhibition at 8, 12 and 16 days for the incorporation of 0.5%, 1.0% and 2.0% of clorexidine respectively, a statistically significant decrease (p<0.001) if compared to the resin without incorporation of chlorhexidine, which showed no inhibitory effect over the biofilm formation.Resinas macias para reembasamento de próteses são largamente utilizadas após cirurgias para estabilizarem a prótese e condicionarem o tecido, aguardando a completa cicatrização. É importante que o material não seja facilmente colonizado por biofilme oral e se possível, evite a contaminação do sítio cirúrgico. Objetivou-se avaliar o efeito da incorporação de clorexidina às resinas acrílicas macias para o reembasamento de próteses totais, através de análises de liberação, citotoxicidade e efeito inibitório de um biofilme de C. albicans. Foram confeccionados corpos de provas (CDPs) com as resinas Trusoft® e Coe-soft®, com incorporação de 0%, 0,5%, 1,0% e 2,0% de clorexidina, totalizando 8 grupos. A liberação de clorexidina foi avaliada através da mensuração da mudança na densidade óptica da solução de armazenamento, na qual ficaram imersos os CDPs, por espectrometria UV, a cada 48 horas, durante 40 dias. A citotoxicidade celular foi avaliada em fibroblastos (linhagem L929), que ficaram 24 horas em contato com meio de cultura no qual os CDPs ficaram previamente imersos, pela técnica de absorção de corante vermelho neutro após 24, 48 e 72 horas e semanalmente até o 28º dia. E, por fim, a atividade antifúngica contra a C. albicans (ATCC 10231) foi avaliada de duas maneiras: (1) teste de difusão em ágar, no qual os CDPs foram colocados em placas de BHI previamente inoculadas com C. albicans, com medição do halo de inibição após 48 horas de incubação a 37ºC; (2) a avaliação da inibição da formação de um biofilme de C. albicans sobre a superfície dos CDPs pela quantificação por metil tetrazólio (MTT) a cada 48 horas, durante 22 dias, com leitura feita em espectrofotômetro de UV. Os dados obtidos foram inseridos no programa SigmaStat (versão 3.1, USA) para realizar as análises estatísticas. As diferenças estatísticas foram determinadas por análises de variâncias do tipo ANOVA e todos os procedimentos para comparações múltiplas pareadas foram feitos utilizando-se o método Holm-Sidak, com nível de significância global igual a 0,05. A clorexidina adicionada às resinas testadas foi capaz de ser liberada para o meio de armazenagem, proporcionalmente à quantidade de clorexidina incorporada, porém com diferentes cinéticas de liberação entre as resinas, visto que a Trusoft libera até 71% do total de clorexidina liberada nas primeiras 48 horas e a Coe-soft, até 44%. Ambas as resinas com incorporação de clorexidina apresentaram efeito citotóxico adicional, se comparadas às resinas sem clorexidina, porém para a Coe-soft não houve diferença estatística dos valores, apenas para a Trusoft (p<0,001). Ocorreu formação de halo de inibição proporcionalmente às concentrações de resinas adicionadas, com maiores halos para a resina Trusoft (p<0,001), e sem formação de halo para as resinas sem clorexidina; a inibição da formação de biofilme, realizada somente com a resina Coe-soft, mostrou total inibição durante 8, 12 e 16 dias, para a incorporação de 0,5%, 1,0% e 2,0% respectivamente, sendo uma diminuição estatisticamente significativa (p<0,001) em relação à resina sem incorporação de clorexidina, que não apresentou inibição do biofilme.Submitted by Boris Flegr (boris@uerj.br) on 2021-01-07T15:06:05Z No. of bitstreams: 1 DISSERTACAO_FINAL_MARTINNA_MENDONCA_BERTOLINI (1).pdf: 904170 bytes, checksum: b2729dc9a257525a3f4ac6da7170fb0c (MD5)Made available in DSpace on 2021-01-07T15:06:05Z (GMT). No. of bitstreams: 1 DISSERTACAO_FINAL_MARTINNA_MENDONCA_BERTOLINI (1).pdf: 904170 bytes, checksum: b2729dc9a257525a3f4ac6da7170fb0c (MD5) Previous issue date: 2011-12-07application/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em OdontologiaUERJBRCentro Biomédico::Faculdade de OdontologiaDenture soft lining materialsBiofilmCandida albicansChlorhexidine diacetateChlorhexidine releaseAntifungal activityResina maciaBiofilmeCandida albicansDiacetato de clorexidinaLiberação de clorexidinaAtividade antifúngicaCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAvaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidinaIn vitro evaluation of two resins-based denture soft lininers modified by chlorhexidine incorporationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALDISSERTACAO_FINAL_MARTINNA_MENDONCA_BERTOLINI (1).pdfapplication/pdf904170http://www.bdtd.uerj.br/bitstream/1/14192/1/DISSERTACAO_FINAL_MARTINNA_MENDONCA_BERTOLINI+%281%29.pdfb2729dc9a257525a3f4ac6da7170fb0cMD511/141922024-02-26 20:13:23.535oai:www.bdtd.uerj.br:1/14192Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T23:13:23Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina |
dc.title.alternative.eng.fl_str_mv |
In vitro evaluation of two resins-based denture soft lininers modified by chlorhexidine incorporation |
title |
Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina |
spellingShingle |
Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina Bertolini, Martinna de Mendonça e Denture soft lining materials Biofilm Candida albicans Chlorhexidine diacetate Chlorhexidine release Antifungal activity Resina macia Biofilme Candida albicans Diacetato de clorexidina Liberação de clorexidina Atividade antifúngica CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina |
title_full |
Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina |
title_fullStr |
Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina |
title_full_unstemmed |
Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina |
title_sort |
Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina |
author |
Bertolini, Martinna de Mendonça e |
author_facet |
Bertolini, Martinna de Mendonça e |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Telles, Daniel de Moraes |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6024021400472077 |
dc.contributor.advisor-co1.fl_str_mv |
Portela, Maristela Barbosa |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7374992387363131 |
dc.contributor.referee1.fl_str_mv |
Figueredo, Carlos Marcelo da Silva |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0411336093247858 |
dc.contributor.referee2.fl_str_mv |
Hirata Júnior, Raphael |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4484092525465200 |
dc.contributor.referee3.fl_str_mv |
Cury, Altair Antoninha Del Bel |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/0850165284804366 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3167449614721645 |
dc.contributor.author.fl_str_mv |
Bertolini, Martinna de Mendonça e |
contributor_str_mv |
Telles, Daniel de Moraes Portela, Maristela Barbosa Figueredo, Carlos Marcelo da Silva Hirata Júnior, Raphael Cury, Altair Antoninha Del Bel |
dc.subject.eng.fl_str_mv |
Denture soft lining materials Biofilm Candida albicans Chlorhexidine diacetate Chlorhexidine release Antifungal activity |
topic |
Denture soft lining materials Biofilm Candida albicans Chlorhexidine diacetate Chlorhexidine release Antifungal activity Resina macia Biofilme Candida albicans Diacetato de clorexidina Liberação de clorexidina Atividade antifúngica CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.por.fl_str_mv |
Resina macia Biofilme Candida albicans Diacetato de clorexidina Liberação de clorexidina Atividade antifúngica |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Denture soft lining materials are widely used after dental surgeries, tissue conditioning and stabilization of prostheses, until the complete tissue healing. It is important that this material not became easily colonized by oral biofilm and if possible, avoid contamination of the surgical site. The aim of this study was to evaluate the effect of chlorhexidine incorporation into resin-based soft denture lining material, considering the drug release analysis, cytotoxicity and C. albicans biofilm inhibition. Specimens were done using Trusoft® and Coe-soft®, incorporating 0%, 0.5%, 1.0% and 2.0% of chlorhexidine, totaling eight groups. The chlorhexidine release was evaluated through the measurement of change in optical density of the storage solution, which the specimens were immersed, by UV spectrometry, after every 48 hours for 40 days. The cellular cytotoxicity was evaluated in fibroblasts (strain L929), which were 24 hours in contact with culture medium, in which the specimens were previously immersed, the technique of neutral red dye uptake was used after 24, 48 and 72 hours and weekly until 28th day. Finally, the antifungal activity against C. albicans (ATCC 10231) was evaluated by two different ways: (1) agar diffusion test, in which the specimens were placed over the top of BHI agar plates previously inoculated with C. albicans, and the measurement of inhibition zone was done after 48h of incubation at 37ºC, (2) C. albicans biofilm inhibition over the specimens surface, which was mensured after every each 48 hours of biofilm and specimens co-incubation, by methyl tetrazolium (MTT), in UV/vis spectrophotometer, during 22 days. Data were analyzed with the SigmaStat software (version 3.1, USA). Statistical differences were determined by analysis of variance ANOVA and all procedures for multiple paired comparisons were made using the Holm-Sidak method, with overall significance level of 0.05. The chlorhexidine added to both resins, Trusoft® and Coe-soft®, can be released to the storage medium, with a dose-related effect, however the resins presented different release kinetics, since the Trusof released up to 71% of the total amount of the chlorhexidine released within the first 48 hours and Coe-soft release only up to 44%, considering the citotoxic effect, both chlorhexidine incorporated resins showed some extra cytotoxic effect, if compared to resins without chlorhexidine, but for Coe-soft no statistical difference of values was founded, only for Trusoft (p<0.001); considering the C. albicans inhibition, the agar diffusion test values were dose-related for both resins, however with bigger inhibition zones for Trusoft (p<0.001), and without any inhibition for the resins without clorexidine. For the C. albicans biofilm inhibition test, performed only with Coe-soft resin, it was verified a complete inhibition at 8, 12 and 16 days for the incorporation of 0.5%, 1.0% and 2.0% of clorexidine respectively, a statistically significant decrease (p<0.001) if compared to the resin without incorporation of chlorhexidine, which showed no inhibitory effect over the biofilm formation. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-12-07 |
dc.date.available.fl_str_mv |
2015-05-05 |
dc.date.accessioned.fl_str_mv |
2021-01-07T15:06:05Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BERTOLINI, Martinna de Mendonça e. Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina. 2011. 54 f. Dissertação (Mestrado em Dentística; Endodontia; Odontopediatria; Ortodontia; Periodontia;) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2011. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/14192 |
identifier_str_mv |
BERTOLINI, Martinna de Mendonça e. Avaliação in vitro de duas resinas macias para reembasamento de próteses modificadas pela incorporação de clorexidina. 2011. 54 f. Dissertação (Mestrado em Dentística; Endodontia; Odontopediatria; Ortodontia; Periodontia;) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2011. |
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http://www.bdtd.uerj.br/handle/1/14192 |
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Universidade do Estado do Rio de Janeiro |
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Programa de Pós-Graduação em Odontologia |
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UERJ |
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BR |
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Centro Biomédico::Faculdade de Odontologia |
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Universidade do Estado do Rio de Janeiro |
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